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Journal : Jurnal Penelitian Saintek

EFFECT OF 2,4-DICLOROFENOKSIASETAT (2,4-D) AND BENZYL AMINOPURIN (BAP) ON THE GROWTH OF BINAHONG LEAF CENTER (ANREDERA CORDIFOLIA L.) AND ANALYSIS OF FLAVONOID TOTAL CONTENT Lili Sugiyarto; Paramita Cahyaningrum Kuswandi
Jurnal Penelitian Saintek Vol 19, No 1: April 2014
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1164.539 KB) | DOI: 10.21831/jps.v19i1.2322

Abstract

The aim of this research was to study the effect of  various concentration of Plant Growth Regulator in MS (Murashige and Skoog) media on callus growth of binahong leaf and total flavonoid content. The method used in the propagation of callus was the leaf explant of binahong with a Completely Randomized Design (CRD). The leaf explants were planted on MS media with different 2,4-D concentrations (1;2;3 ppm), 0,5ppm IBA+0,5ppm BAP; 0,5ppm IBA+1,0 ppm BAP ; 1,0 ppm IBA+0,5 ppm BAP, each with 15 repetition. The parameters observed in this research were initiation time, type, colour, diameter, the number of callus and total flavonoid content.  The result showed that the optimum growth of callus is at 3 weeks and after  that it declined or stayed stagnant. The result of the analysis of variance (ANOVA) showed that there is no significant difference in the media used in this research. The total flavonoid content of fresh leaf sample  is higher than callus sample.