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All Journal Jurnal Perikanan dan Kelautan Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Celtic Al-Iqtishad : Jurnal Ilmu Ekonomi Syariah (Journal of Islamic Economics) Journal of Engineering and Technological Sciences JURNAL PENGABDIAN KEPADA MASYARAKAT Edu Geography Jurnal Pendidikan Jasmani Kesehatan dan Rekreasi (Penjaskesrek) Animal Production : Indonesian Journal of Animal Production Jurnal SOLMA Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) Jurnal Ketahanan Pangan Majalah Ilmiah Pengkajian Industri SeBaSa: Jurnal Pendidikan Bahasa dan Sastra Indonesia Bahasa: Jurnal Keilmuan Pendidikan Bahasa dan Sastra Indonesia Jurnal Pengembangan Penyuluhan Pertanian Journal Sensi: Strategic of Education in Information System Riset: Jurnal Aplikasi Ekonomi Akuntansi dan Bisnis Jurnal Cahaya Mandalika Jurnal Kependidikan: Jurnal Hasil Penelitian dan Kajian Kepustakaan di Bidang Pendidikan, Pengajaran dan Pembelajaran Jurnal Pengabdian Kesehatan Komunitas (Journal Of Community Health Service) Indo-MathEdu Intellectuals Journal Journal of Computer Science and Information Systems (JCoInS) Eirene Jurnal Ilmiah Teologi Prosiding University Research Colloquium Journal of Innovation and Research in Primary Education Sapientia Humana : Jurnal Sosial Humaniora EDUCTUM: Journal Research Studies in English Language and Education PEDADIDAKTIKA: Jurnal Ilmiah Pendidikan Guru Sekolah Dasar Jurnal Kedokteran Hewan Jurnal Manajemen Sistem Informasi SENAPAS Jurnalistrendi: Jurnal Linguistik, Sastra dan Pendidikan i-Plural Jurnal Pemikiran dan Pengembangan Pembelajaran
Kurniasih Kurniasih
Doctoral Program of Environmental Engineering, Faculty of Civil and Environmental Engineering, Institut Teknologi Bandung
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Journal : Animal Production : Indonesian Journal of Animal Production

 1 
Lactic Acid Bacteria Isolated from the Gastro-Intestinal Tract of Chicken: Potential Use as Probiotic Harimurti, S; Nasroedin, ES; Nasroedin, Nasroedin; Kurniasih, Kurniasih
ANIMAL PRODUCTION Vol 9, No 2 (2007): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Lactic acid bacteria have been suggested to have several beneficial effects on human and animals.  These bacteria, indigenous to the gastro-intestinal tract, are important in regulating the balance among the desirable and undesirable intestinal microflora and in controlling enteric pathogenic infection in the host.  Objectives of this research are to obtain lactic acid bacteria isolates from gastro-intestinal tract of chicken and to screen their ability as a probiotic agent i.e., their antagonistic against pathogenic bacteria, their survival at low pH and high concentration of bile salt.  In this research, 74 samples used as sources of bacteria, and among them only 11 samples could be isolated as lactic acid bacteria with the total number of isolates of 61.  Based on the preliminary screening i.e., their antagonistic factor against pathogenic bacteria, 20 isolates was further studied.   Based on the identification scheme, these isolates belong to three species, i.e., Lactobacillus murinus, Pediococcus acidilactici, and Streptococcus thermophilus. The result showed that most isolates grow well in the media with the initial pH of 5.5, but their growth were retarded when the initial pH 3.5.  Only one isolate Streptococcus thermophilus Kp-2 showed its growth at initial pH of 3.5.   All isolates did not show any growth at initial pH 2.5, though their viability still high. The result based on the isolates resistance to bile salt showed that most isolates could grow at media with 0.20% of bile salt.  Their growth was inhibited with the increasing bile salt concentration.  However, few isolates could grow well at media with 1% of bile salt. Based on their characteristics three isolates i.e., Lactobacillus murinus Ar-3, Streptococcus thermophilus Kp-2, and Pediococcus acidilactici Kd-6 were selected as probiotic agents for the continuing research. i.e. production of biomass and its application to chicken production. (Animal Production 9(2): 82-91 (2007) Key Words : Lactic acid bacteria, gastro- intestinal tract of chicken, probiotic agents
Identification of Excretory – Secretory Sporozoite Antigens of Eimeria tenella with Monoclonal Antibody Prastowo, J; Nurcahyo, W; Kurniasih, Kurniasih; Wasito, R
ANIMAL PRODUCTION Vol 7, No 2 (2005): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

The research done specifically to identified the. Eimeria tenella excretory-secretory sporozoit antigen by using the monoclonal antibody reaction instead. Eimeria tenella excretory-secretory sporozoit antigen. The 107 sporozoits were obtained from existation of 5x107 oocysts with 25 ml tripsin and sodium taurocholat. Excretion - secretion antigen were preparated by freezing and thawing and then be isolated with ice bath 20 second, 20 meter of amplitude as the further treatment.  Determinating the excretion -secretion sporozoit protein by using BCA-test. While the visualization sporozoit protein antigen by SDS-PAGE. Monoclonal antibody were produced through hybridization between B BALB/c limphosit immunized with Eimeria tenella excretion-secretion sporozoite antigen and myeloma cells. The monoclonal antibody identification  of excretion – secretion antigen were done through western blott. The visualization of protein molecule weight of Eimeria tenella sporozoit were resulted 20 protein fraction, included 14, 16, 17, 18, 20, 24, 26, 32, 34, 36, 39, 43, 47, 56, 65, 67, 76, 87, 94, and 96 KDa. There were 12 kinds of monoclonal antibody could be produced from these proteins. While the 5 hybridoma immunoblotting resulted specific reaction by the appearances of reaction ribbon obviously, there were MABset 1 which identified protein epitop with 14.7 KDa of molecule weight,  MAbset 2 which identified protein epitop with 43 KDa of molecule weight, MAbset 3 which identified protein epitop with 42 KDa of molecule weight, MAbset 4 which identified protein epitop with 47 KDa of molecule weight and MAbset 5 which identified protein epitop with 90 KDa of molecule weight. As the conclusion, five of excretion-secretion sporozoit antigen were proper immunogen to stimulate the hospest immunity. (Animal Production 7(2): 95-100 (2005) Key Words: Sporozoit, Antigent, Eimeria tenella, Coccidiosis
Co-Authors A.A. Ketut Agung Cahyawan W Ahmad Abdul Karim, Ahmad Abdul Ajie, Sophan Alaydrus, Yahya Amanda, Kevin amin, makomulamin Aminudin Sulaeman Andera, Ayu Anggraini, Merliyani Putri Ani Hendriani, Ani Arie Rakhmat Riyadi Asdianti, Rinda Atik Umamah Azizah, Amalia Nurul Cahyana, Misan Chetrine, Ema Darwis, Muh Diah Retno Widowati Dyah Maya Nihayah EDWAN KARDENA Eko Suhartoyo, Eko Endah, Lely ES Nasroedin, ES Fiddaraini, Hasana Fransiskus, Ferdinan Ginting, Lea Christina Br Hartati, Dian Henny Rahmawati Herry Mulyono Herto Dwi Ariesyady, Herto Dwi Idris Moh Latar Iesje Lukistyowati Ika Hidayanti J Prastowo, J Joko Prastowo Kiromah, Naelaz Zukhruf Wakhidatul Kurniawan, Rano Kusumapradja, Rokiah Lutfa, Prasyifa Khabibatul M. Affandi Arianto Magistra, Ari Arasy Maideshinta, Tri Kurnia Mela Darmayanti Mintarsi, Mintarsi Mubarokah, Wida Wahidah Muhamad Yusup Mulyana, Topik Mulyati Mulyati Muttaqin, Alfin Nasihah, Durotun Nasroedin Nasroedin, Nasroedin Novita, Rini Nunuy Nurjanah Nur Mukminatien Pranata, Heru Prihamdani, Ferju Priyo Sambodo Putri, Harmida Gusnia R Wasito Rahmati, Nuse Aliyah Ramadhany, Eka Putri Ratih Novita Sari, Ratih Novita Retty Isnendes Rifai , Andi Anna Rifka Fadilah, Rifka Rizqon Halal Syah Aji, Rizqon Halal Rosdiana Rosdiana RR. Ella Evrita Hestiandari S Harimurti, S Sari, Putri Yunita Sari, Rezqina Saryati, Titi Sekar, Sabila Shoimatun Febriyani, Shoimatun Sihombing, Edy Syahputra Siti Narwiyani Soedarmanto Indarjulianto SRI RAHAYU SRI WARDANI SUDARMAJI SUDARMAJI Sukmawati, Ita Sumitro Sumitro Suparno Suparno Supriyanto, Sugeng Sutarno, Alfonsus Syaripudin, Tatang Tahapary, Johni Melvin Ulfa, Zuriyati W Nurcahyo, W Wahyudi K, Imam Widiarso, Budi Purwo Widiarso, Widiarso, B. P. Widiastuti, Tri Cahyani Widowati, Diah Retno Winci Firdaus Wisnu Nurcahyo