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Armaini -
Andalas University
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Other

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Journal : Jurnal Riset Kimia

 1 
OPTIMASI NUTRISI MEDIA PERTUMBUHAN BAKTERI TERMOFIL PENGHASIL SELULASE DARI SUMBER AIR PANAS RIMBO PANTI -, Armaini; Dharma, Abdi; Syukur, Sumaryati; -, Jamsari
Jurnal Riset Kimia Vol 5, No 1 (2011): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v5i1.165

Abstract

 Optimization have been done on the media for the growth of the isolated thermophiles bacteria from hot springs Rimbo Panti, the nutrients comprising variety of carbon sources such as CMC (carboxymethyl cellulose), avicell (micro crystalline cellulose), and cellobiose, with a variety of sources organic nitrogen, peptone, extracts yeast, tryptone, and urea, as well as variations consist of inorganic nitrogen sources, KNO3, NaNO3, (NH4)2SO4, and (NH4)NO3. Determination of cellulase activity performed using DNS reagent (3,5-dinitro salicylic acid). Maximum cellulase production with high activity based on the results of this research, the best of carbon source is CMC with optimum concentration 0.125%, inorganic nitrogen source is peptone with the optimum concentration of 0.3 to 0.4% and the inorganic nitrogen source is (NH4)2SO4 with optimum concentration of 0.2 - 0.25%. Optimization of size of inoculums obtained the optimum amount of inoculums 2%. Keywords: Optimization, thermophiles bacteria, cellulose, carbon sources, nitrogen sources
OPTIMASI NUTRISI MEDIA PERTUMBUHAN BAKTERI TERMOFIL PENGHASIL SELULASE DARI SUMBER AIR PANAS RIMBO PANTI Armaini -; Abdi Dharma; Sumaryati Syukur; Jamsari -
Jurnal Riset Kimia Vol. 5 No. 1 (2011): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v5i1.165

Abstract

 Optimization have been done on the media for the growth of the isolated thermophiles bacteria from hot springs Rimbo Panti, the nutrients comprising variety of carbon sources such as CMC (carboxymethyl cellulose), avicell (micro crystalline cellulose), and cellobiose, with a variety of sources organic nitrogen, peptone, extracts yeast, tryptone, and urea, as well as variations consist of inorganic nitrogen sources, KNO3, NaNO3, (NH4)2SO4, and (NH4)NO3. Determination of cellulase activity performed using DNS reagent (3,5-dinitro salicylic acid). Maximum cellulase production with high activity based on the results of this research, the best of carbon source is CMC with optimum concentration 0.125%, inorganic nitrogen source is peptone with the optimum concentration of 0.3 to 0.4% and the inorganic nitrogen source is (NH4)2SO4 with optimum concentration of 0.2 - 0.25%. Optimization of size of inoculums obtained the optimum amount of inoculums 2%. Keywords: Optimization, thermophiles bacteria, cellulose, carbon sources, nitrogen sources
Evaluation of Lipid Profile and Liver Function After Administration of Scenedesmus dimorphus in Obese Mice Armaini Armaini; Siti Hajir; Yetria Rilda
Jurnal Riset Kimia Vol. 13 No. 2 (2022): September
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jrk.v13i2.503

Abstract

Obesity is a major public health problem in developing countries and is a significant risk factor for metabolic disorders. Microalgae Scenedesmus dimorphus (S. dimorphus) contains bioactive compounds such as pigment function as antioxidants, and omega-3 and omega-6 PUFAs have potential as nutraceuticals. The study aimed to evaluate the lipid profile and liver function after the administration of S. dimorphus in obese mice. The research design uses mice which are divided into 6 groups; Group 1 (G1) normal control, G2 control Obesity, G3 treatment with Orlistat, G4 treatment S. dimorphus (0.25mg/g BW), G5 treatment S. dimorphus (0.5 mg/g BW) and G6 treatment S. dimorphus (0.75 mg/g BW) each group consisted of 5 mice and 21 days of observation time. The parameters observed were lipid profile and liver function of mice. Based on the results of the study, the effective dose for treating obesity is a dose of S. dimorphus 0.75 mg/g (BW) can reduce cholesterol, triglycerides, and LDL levels, respectively 67.7 mg/dl, 49.2 mg/dl, 10 , 2 mg/dl, and increased HDL, 68.32 mg/dl compared to control of obesity (G2), respectively 108.7 mg/dl, 139.1 mg/dl, 20.6 mg/dl and HDL 60, 28 mg/dl, this dose is also effective for improving the function of blood pressure by reducing AST and ALT 15.6 U/L and 18.8 U/L, respectively, compared to the obesity group (G2), which is 26.6 U/L, and 29,7 U/L. Based on the results of the study it can be concluded that S. dimorphus is useful for anti-obesity for mice (Mus musculus).
Co-Authors Abdi Dharma Afriyanti Azhar Indriani Sahira Jamsari Jamsari Nasril Nasir Nessa Satya Wati Rahmiana Zein Riska Hernandi Siti Hajir Sumaryati Syukur Syafriza Yanti Tedy Alfajri Tio Putra Wendari Upita Septiani Yetria Rilda Zulkarnain Chaidir