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All Journal Jurnal Ilmu Pertanian Indonesia Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Kesehatan Andalas Jurnal Teknik Sipil BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Kimia Terapan Indonesia ANNALES BOGORIENSES OLDI (Oseanologi dan Limnologi di Indonesia) Marine Research in Indonesia Jurnal Riset Biologi dan Aplikasinya Pharmaceutical Sciences and Research (PSR) Makara Journal of Science Annales Bogorienses
PUSPITA LISDIYANTI
Pusat Penelitian Bioteknologi, Lembaga Ilmu Pengetahuan Indonesia (LIPI)
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

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Journal : Annales Bogorienses

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EDITOR'S PREFACE Lisdiyanti, Puspita
Annales Bogorienses Vol. 23 No. 1 (2019): Annales Bogorienses
Publisher : BRIN

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EDITOR'S PREFACE Lisdiyanti, Puspita
Annales Bogorienses Vol. 22 No. 1 (2018): Annales Bogorienses
Publisher : BRIN

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EDITOR'S PREFACE Lisdiyanti, Puspita
Annales Bogorienses Vol. 22 No. 2 (2018): Annales Bogorienses
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EDITOR'S PREFACE Lisdiyanti, Puspita
Annales Bogorienses Vol. 26 No. 1 (2022): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2022.v26.n1.i-vii

Abstract

A Preliminary Report on The Syntheses of Oligonucleotide Primers in The National Research and Innovation Agency (NRIA) Atikana, Akhirta; Prasetyoputri, Anggia; Rubiyana, Yana; Herawati, Neng; Desriani, Desriani; Pratiwi, Riyona Desvy; Wulandari, Dwi; Sukmarini, Linda; Kusharyoto, Wien; Santoso, Adi; Putra, Masteria Yunovilsa; Lisdiyanti, Puspita
Annales Bogorienses Vol. 26 No. 1 (2022): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2022.v26.n1.21-27

Abstract

A PolyGen DNA Synthesizer is equipment that is used for synthesizing oligonucleotide primers for any amplification targets. Oligonucleotide primers are indispensable components for any Polymerase Chain Reaction (PCR)-based detections. In the present study a number of oligonucleotide primer sets were synthesized to target (1) the Human Insuline Glargin (HIG) and (2) the Human Erythropoietin (EPO), as well as (3) the RNA-dependent RNA Polymerase (RdRp) and (4) the Nucleocapsid (N) genes of the severe acute respiratory syndrome virus 2 (SARS-CoV-2). A solid-phase oligonucleotide synthesis method was used according to the default protocol of the Polygen’s instrument to synthesize primers at a 40 nmol scale. The synthesized primers in this study were compared to commercially produced primers in their ability to amplify the gene target(s) in PCR and quantitative real-time PCR (qPCR) reactions. The first two sets of primers showed similar results in PCR compared to commercial primers; however, these primers were not tested for qPCR due to sample limitation. In contrast, the primer sets 3 and 4 were not able to produce amplicons in PCR reactions and only the primer set 4 successfully amplified the gene target in qPCR. These results indicate that the crude primers synthesized in this study are promising candidates for molecular detection and diagnostics, but these primers would benefit from further optimization for routine applications.
Antimicrobial Activities of Actinobacteria Isolated from Marine and Terrestrial Samples in Lombok Island, Indonesia Ratnakomala, Shanti; Krause, Janina; Sari, Miranti Nurindah; Kusumawardhani, Dinihari Indah; Kusmiati, Mia; Lisdiyanti, Puspita
Annales Bogorienses Vol. 26 No. 1 (2022): Annales Bogorienses
Publisher : BRIN

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/ann.bogor.2022.v26.n1.28-36

Abstract

During our study on exploring actinobacteria from Indonesian sources, we selected 59 strains of actinobacteria isolated with five different isolation methods from Lombok Island Indonesia. The aim of this research was to isolate and characterize those actinobacteria, and to screen the microbial activities against 5 bacterial tested. Six soil samples from Lombok Island were processed. Totally, 59 isolated were selected. Of these strains, 31 strains showed antibiotic activity. Seventeen strains from 59 strains have been identified by 16S rRNA gene sequencing, and most dominant of the strains belongs to the genus Streptomyces. Three of them were chosen for further experiments due to their broad activity against four of five test strains. From two strains, at least one bioactive substance could be extracted. As only few bioactive substances could be extracted with ethyl acetate from liquid cultures more experiments should be performed on this subject. More solvent extraction should be tested as well as other production media. In conclusion, present study did now reveal bioactive actinobacteria were isolated from soil samples in Lombok Island. Antimicrobial compound has also been detected which is active against a broad range of pathogens. These findings may have an importance to produce potentially new antibiotic substances from actinobacteria indigenous Indonesia.
Co-Authors - Yopi Abd. Rasyid Syamsuri ADE ANDRIANI Adi Santoso Agustinus Robert Uria Ahmad Thontowi Akhmad Rivai ANDI UTAMA Andi Utama ANDO KATSUHIKO Apriliana, Pamella Ariani Hatmanti Ario Betha Juanssilfero Ashadi Sasongko Atikana, Akhirta Caniago, Asnany Caniago, Asnany Desriani Desriani Desriani Desriani Dewi Seswita Zilda dinihari indah kusumawati Dwi Wulandari Elvi Yetti Endang Saepudin, Endang Endang Sukara Erdiansyah Rezamela, Erdiansyah Fahrurrozi Fahrurrozi Fahrurrozi Fahrurrozi GINA KARTINA Herman Suryadi JAKA WIDADA KATSUHIKO ANDO Krause, Janina Kusharyoto, Wien Kusharyoto, Wien Kusumawardhani, Dinihari Indah Kusumawati, Dinihari Indah Kusumawati, Dinihari Indah Lina Elfita Lina Elfita LINDA SUKMARINI M. Iqbal Prawira-Atmaja, M. Iqbal Mangunwidjaja, Wibowo MARIA BINTANG Maulidia, Faiqoh Nur Meliah, Siti Meliah, Siti Mia Kusmiati Miranti Nurindah Sari, Miranti Nurindah MISA OTOGURO Mochammad Imron Awalludin Mulyorini Rahayuningsih Nadhifah, Hana NANIK RAHMANI Nanik Rahmani Neneng Hasanah Neng Herawati, Neng Ni Luh Putu Vidya Paramita Nurul Fitri Sari Nuryana, Isa Prasetyoputri, Anggia Pratama, Rendy Eka Prayoga, Muhammad Bagas Putra, Filemon Jalu Nusantara Putra, Masteria Yunovilsa Putri, Ade Lia Putri, Ade Lia Restu Wulansari, Restu Riyona Desvy Pratiwi, Riyona Desvy ROHMATUSSOLIHAT ROHMATUSSOLIHAT RONI RIDWAN Seprianto, Seprianto Shanti Ratnakomala SHANTI RATNAKOMALA Shanti Ratnakomala Siti Meliah Subagus Wahyuono Suharsono Suharsono Sukma Nuswantara Susanti, Agustina Eko Syafriana, Vilya Tisnadjaja, Djadjat Tisnadjaja, Djadjat Titik Nuryastuti TOMOHIKO TAMURA Toto Iswanto TRI RATNA SULISTIYANI Triana Hertiani Trisanti Anindyawati, Trisanti Tutik Murniasih Ukhradia Maharaniq Safira Untari, Febriana WIBOWO MANGUNWARDOYO Yana Rubiyana, Yana Yantyati Widyastuti Yopi Y Yopi Yopi YOPI YOPI Yopi, YULIN LESTARI Yusro Nuri Fawzya