Hapsari Mahatmi
Laboratorium Bakteriologi Dan Mikologi Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia, 80234

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Journal : Jurnal Ilmu dan Kesehatan Hewan

Resistensi Bakteri Aeromonas sp. Isolat Ikan Piranha (Pygosentrus nattereri) Bali Safari And Marine Park terhadap Antibiotik Ni Made Herawati; Hapsari Mahatmi; I Nengah Kerta Besung
Veterinary Science and Medicine Journal Vol 1 No 2 (2013)
Publisher : Udayana University

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Abstract

Piranha (Pygosentrus nattereri) is a fish that has been conserved at Conservation Center of Bali Safari and Marine Park. In May 2012 there was a case that twelve Piranhas suffered from illness and two of them died without any specific clinical signs. The dead Piranhas were necropsied and the tissues or parts of organs were collected for microbial examination interests. Spesies specific bacterial examination was also included using Microgen GNA TM + B-ID system test The bacterial examination found that the fishes were infected by Aeromonas hydropila and Aeromonas veronii. Further examination about antibiotic resistance tests showed that those Aeromonas spp were resistant to chloramphenicol and a combination of trimethoprim and sulphamethoxazole. It can be suggested that, next, the usage of other antibiotics is strongly recommended for the case of Piranha infected by Aeromonas spp at Bali Safari and Marine Park.
Pola Resistensi Salmonella typhi yang Diisolasi dari Ikan Serigala (Hoplias malabaricus) terhadap Antibiotik Waode Santa Monica; Hapsari Mahatmi; Kerta Besung
Veterinary Science and Medicine Journal Vol 1 No 2 (2013)
Publisher : Udayana University

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The purpose of this study was to know the resistant pattern of Salmonella typhi (S. typhi) isolated from wolf fish (Hoplias malabaricus) to antibiotics in Bali Safari and Marine Park. S. typhi was isolated from fecal swab collected from the illness fish. The S. typhi was confirmed with MicrogenTM GN A ID test’s method. The antibiotic sensitivity of the S. typhi was tested using streptomycin, gentamicin, penicillin, vancomycin, and chloramphenicol according to the Kirby bouer method. The results showed that the isolate of S. typhi was resistant to penicillin and vancomycin, but sensitive to streptomycin, gentamicin, and chloramphenicol.
Detection of Mycobacterium bovis and Klebsiella pneumoniae at Bali Cattle Slaughterhouse by culture analysis and PCR I Gede Oka Darsana; I Nyoman Dibia; Hapsari Mahatmi
Veterinary Science and Medicine Journal Vol 3 No 2 (2015)
Publisher : Udayana University

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A study to determine the presence and prevalence of bovine tuberculosis and Klebsiella pneumoniae atcattle slaughterhouse in Bali was carried out in Pesanggaran and Mambal abattoirs from January to March2015.The Lungs and lymph nodes were inspected for lesions and then examined through DNA analysis forMycobacterium bovis and culture for isolation of Klebsiella pneumoniae. Of the samples examined, no onehad lesions suggestive of tuberculosis in two abattoirs.However, Klebsiella pneumoniae was observed1/4513 sample (0.02%) only in Mambal abattoir. This study is the first report of the presence of Klebsiellapneumoniae in Bali. Therefore, a aware of this zoonotic Klebsiellosis and its control managementformulation are need.
Deteksi Antibodi Mycobacterium tuberculosa bovis pada Sapi di Wilayah Kabupaten Buleleng, Bangli, dan Karangasem Provinsi Bali Putu Gede Widiarsa Putra; Nengah Kerta Besung; Hapsari Mahatmi
Veterinary Science and Medicine Journal Vol. 1 No. 1 2013
Publisher : Udayana University

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Bovine tuberculosis (BTB) is one the most widespread zoonotic diseases and has recently reemerged as a major health concern. The disease is caused by the Mycobacterium tuberculosis complex (MTBC) that primarily affects humans as its main host, and that is able to attack more hosts such as domestic animals, pet animals, and wild animals. This Cross-Sectional Study was carried out to determine the seroprevalence of BTB in Districts of Buleleng, Bangli, and Karangasem Province of Bali. One hundred and eighty cow blood samples were collected, and the serological status of BTB was detected using Elisa method. The results showed that the seroprevalence of BTB in District of Bangli was 2.22%, while, those was negative in Districts of Buleleng and Karangasem. The positive seroprevalence of BTB in Bangli indicates that the same research must be conducted throughout the districts in the Province of Bali.
Kepekaan Eschericia coli Patogen yang Diisolasi dari Babi Penderita Kolibasilosis terhadap Antibiotik di Kecamatan Kerambitan dan Tebanan Kabupaten Tabanan, Bali Aditya Nugraha; NK. Besung; Hapsari Mahatmi
Veterinary Science and Medicine Journal Vol 1 No 2 (2013)
Publisher : Udayana University

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This study aims to determine the sensitivity pattern of E. coli isolated from pigs with colibacillosis to antibiotics. The total of 21 fecal samples was collected from pigs with white scour at the District of Kerambitan and Tabanan, the Regency of Tabanan, Bali. The isolation and identification of pathogenic E coli used Sheep Blood Agar medium, and serotype grouping of those bacteria was designated through agglutination test using serum’s antibody Polyvalent 1-5 and 6-11. The pathogenic E. coli found was then examined it`s own resistance to streptomycin, sulfamethoxazole, gentamicin, cefotaxime, and chloramphenicol according to Kirby Bouer method. The result showed that the isolates of E. coli polyvalent 1-5 were resistant to streptomycin and chloramphenicol, whereas that of polyvalent 6-11 was resistant to sulfamethoxazole, gentamicin, streptomycin and chloramphenicol.