Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal Jurnal Akademika Biologi Pro-Life BIOMA
Siti Muflichatun Mardiati
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology

Published : 7 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search
Journal : Jurnal Akademika Biologi

 1 
HEPATOSOMATIC INDEX (HSI) DAN DIAMETER HEPATOSIT MENCIT (Mus musculus L.) SETELAH PAPARAN EKSTRAK AIR BIJI PEPAYA (Carica papaya L.) Perwitasari Wahyuningtyas; Agung Janika Sitasiwi; Siti Muflichatun Mardiati
Jurnal Akademika Biologi Vol. 7 No. 1 Januari 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.951 KB)

Abstract

Papaya seeds were one part of the papaya plants that can be used as a traditional medicine. The purpose of this study was to test the effect of papaya seed extract (Carica papaya L.) to Hepatosomatic Index (HSI) and hepatocytes diameter of mice (Mus musculus L.). This study was an experimental study use Completely Randomized Design (CRD) that divided into 5 treatment groups with 3 replications. P0 test material in the form of aquadest, P1, P2, P3 in the form of papaya seeds extract test with dose respectively 1.4; 3.5; And 7 mg / mL. Treatment were administered orally for 21 consecutive days. The Body weights of mice were measured every 7 days. After treatment were terminated, the body weights of mice were measured and then sacrificed by dislocation of the neck and the liver were taken by surgery. Furthermore, the liver weights were measured and made histological preparations with HE staining. The determination of HSI were obtained from liver weights and body weights. The measurement of hepatocytes diameter were done by using photomicrograph at each incision 3 field of view. Data were analyzed by Analysis of Variance (ANOVA) at 95% confidence level. The analysis result of HSI in the treatment group showed that there were no significant difference (p>0,05). The analisys of hepatocytes diameter of the treatment group showed significant difference (p<0.05). It could be concluded that exposure of papaya seeds extract for 21 days was a reversible effects so that the hepatocytes diameter size of mice increases but didn’t changed the HSI.Keywords: Carica papaya, HSI, hepatocytes diameter
PERBAIKAN HISTOLOGIS PUSAT LEARNING AND MEMORY DI HIPOKAMPUS OTAK SETELAH PERLAKUAN SUPLEMEN DAGING IKAN GABUS (CHANNA STRIATA) DALAM PAKAN: KAJIAN IN VIVO PADA TIKUS WISTAR PASCA STRES Reynato Wijaya Saputra; S Sunarno; Siti Muflichatun Mardiati
Jurnal Akademika Biologi Vol. 5 No. 3 Juli 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (293.993 KB)

Abstract

Background: Stress due to lack of feed that followed excessive activity is a trigger factor learning and memory disorder center in the hippocampus. Cornu ammonis (CA3 and CA1) in the hippocampus is a structure that is central to learning and memory. Complete nutrient content of feed is supporting the regeneration of the structure of CA3 and CA1 in the hippocampus impaired due to stress . This research was conducted to obtain a histological improvement layers of CA3 and CA1 hippocampus in the rat brain after supplementation with snakehead fish meat in the  feed of post-stress.Design and Method: This study uses a completely randomized design consisting of                 6 treatments with 4 replications. Test animals used were male Wistar rats. Treatments, including P0: control, namely Wistar rats were conditioned to stress during the six days that followed the feeding without supplementation of snakehead fish meat for 14 days; P1, P2, P3, and P4, respectively Wistar rats were conditioned stress followed by supplemental feeding snakehead fish meat 5%, 10%, 15% and 20%. The research variables measured the thickness of the layer of CA3 and CA1. Data were analyzed using Analysis of Variance (ANOVA) at the level of 95% and continued with Duncan test with 95% significance level .Result: Supplemental feeding with snakehead fish meat as much as 15% during 14 days in Wistar rats after stress gives the best effect on the improvement of CA3 and CA1 layer of the hippocampus, respectively 53.60 μm2 (17.49%) and 46.31 μm2 (78,32%), better than the control.Conclusion: Feeding the snake head fish meat supplementation for 14 days in Wistar rats after stress can improve layer CA3 and CA1 in the hippocampus of the brain. Recommended snake head fish meat can be used as a feed supplement to improve the structure of CA3 and CA1 in the hippocampus and very good to support the learning and memory functions of the brain. Keywords: snakehead fish meat, Channa striata, cornu ammonis, supplementation, hippocampus, learning and memory
TINGKAH LAKU MAKAN DAN MINUM ITIK MAGELANG (Anas javanica) SETELAH PEMBERIAN CAHAYA MERAH DAN PUTIH SERTA KURKUMIN DALAM PAKAN Martha Raras Vegasari; Siti Muflichatun Mardiati; Enny Yusuf Wachidah Yuniwarti
Jurnal Akademika Biologi Vol. 7 No. 1 Januari 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (80.12 KB)

Abstract

Magelang duck was one of the most productive local poultry as an egg producer. Improvements in the management of aves cultivation could be done with the addition of curcumin and light. Curcumin and light were able to affect the behavior of feeding and drinking. This study aimed to analyze the increases of feeding and drinking behavior of magelang duck (Anas javanica) which was fed with curcumin with red and white lighting. This study used 12 female ducks that were 17 weeks old. The study consisted of 4 groups: A0B0 (white light and curcumin 0 mg), A0B1 (white light and curcumin 18 mg), A1B0 (red light and curcumin 0 mg), and A1B1 (red light and curcumin 18 mg). The parameters observed in this study were the frequency of feeding and drinking of magelang ducks (Anas javanica). This research was analyzed by descriptive method. The conclusion of the study was that the giving of red light and white light and curcumin in the curcumin has not potential to improve the duck feeding activity in the stationary phase, while giving white light has potential to improve the behavior of drinking of magelang ducks.Key words: feeding and drinking behavior, Magelang duck, curcumin, red and white light.
EFEKTIVITAS PAKAN DENGAN SUPLEMENTASI DAGING IKAN GABUS (Channa Striata) TERHADAP PERBAIKAN HISTOLOGIS DUODENUM TIKUS WISTAR SETELAH MENGALAMI PENGONDISIAN STRES FISIOLOGIS Rudi Juandi Gultom; S Sunarno; Siti Muflichatun Mardiati
Jurnal Akademika Biologi Vol. 5 No. 2 April 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (306.145 KB)

Abstract

Physiological Stress and nutritional deficiencies that followed excessive activity is a trigger factor disturbance structure and function of the duodenum. Organ in the duodenum has the ability to regenerate periodically after experiencing problems due to physiological stress. Nutrient content of feed determine organ regeneration process duodenum of the small intestine. This study was conducted to assess, analyze and determine the concentration of fish meat cork to increase in diameter, villous length, the thickness of the layer of the mucosa, submucosa and muscularis duodenum of Wistar rats after conditioning the physiological stress.This study used 20 male Wistar rats consisted of 5 treatments with 4 replications. The treatment in this study consisted of P0: control, test animals were conditioned stress followed six days of feeding without supplementation with fish meat cork; P1, P2, P3, and P4 are test animals that were conditioned stress for 6 days and fed with supplementation of fish meat cork for 14 days, respectively 5%, 10%, 15%, and 20% of the time after conditioning stress. This study uses a completely randomized design (CRD). The parameters measured in this study is the diameter, villous length and thickness of the layers of mucosa, submucosa, and muscularis duodenum of the small intestine. Data were analyzed using Analysis of Variance (ANOVA) followed by Duncan test with 95% significance level. The results of this study are feeding the fish meat cork can improve the structure of histologic duodenum after  stress conditioning and feeding with supplementation of fish meat cork with a concentration of 15% giving effect to increase in diameter, the length of villi, and the thickness of the lining mucosa, submucosa, and muscularis duodenum, respectively -turut 2520 μm, 576.34 μm, 506 μm, 123.34 μm and 72.34 μm respectively higher 23.43%, 46.37%, 45.26%, 50.31%, and 22 , 95% compared to controls. It concluded supplementation with fish meat cork in feed can be used to improve the structure and function of post-stress duodenum.Keywords: stress, supplements, duodenum, cork fish, Channa striata, Wistar
Co-Authors Agung Janika Sitasiwi Cecep Kusmana Enny Yusuf Wachidah Yuniwarti Martha Raras Vegasari Perwitasari Wahyuningtyas Reynato Wijaya Saputra Rudi Juandi Gultom Rudy Juandi Goeltoem S Sunarno Sunarno Sunarno