The ejaculation fraction on Etawa crossbred goat (PE goat) has various standard spermatozoa qualitues, one of which ismotility of spermatozoa. The motility of spermatozoa is very important to note in the development of artificial insemination techniques, because the success in artificial insemination techniques is strongly influenced by the motility of spermatozoa. The motility of semen spermatozoa is known through the process of examining the movement of spermatozoa in microscopic testing. The aim of this research was find out the effect of ejaculation fraction on motility of PE goat spermatozoa. Using an experimental method with a group's random consisting of two treatment groups; K1 is fraction 1and K2 is a fraction 2. The Replication of each treatment was eight replications and a total of sample research was 16 unit. The semen was collected once a week using artificial vagina (VA) technique. The semen collecting until the second ejaculation and motility test of spermatozoa were immediately measured use sperm vision analyzers IVOS II. The test results of the ejaculation fraction observation using an independent sample t-test showed the value of t hit = 0.700 and t (0.025) than 2.14 t hit