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All Journal Jurnal Ilmu Kefarmasian Indonesia Parapemikir Jurnal Ilmiah Farmasi
Hendig Winarno
Universitas Pancasila
Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Physics Public Health

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Journal : Jurnal Ilmu Kefarmasian Indonesia

 1 
Isolasi dan Identifi kasi Senyawa Kimia Zat Anti Kanker dari Daun Kopasanda (Chromolaena odorata (L.)) MUHAMMAD FITRAH; HENDIG WINARNO; PARTOMUAN SIMANJUNTAK
JURNAL ILMU KEFARMASIAN INDONESIA Vol 15 No 1 (2017): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Isolation and identifi cation of chemical compound as anticancer from the leaves of Kopasanda (Chromolaena odorata L.) had been done. Extraction was conducted successfully with n-hexane, ethylacetate and ethanol by maceration, and by refl ux for water solvent. Fractionation of ethylacetate extract by column chromatography (SiO2). n-hexane-ethylacetate = 10:1 ~ 1:1; ethylacetate). n-hexaneethylacetate = 5:1) gave one pure isolated fraction fr. 5-3 with inhibition activity against leukemia cell line L1210 at concentration 1.515 ppm. Chemical structure determination was done based on spectral data interpretation of Infra Red, 1D NMR (1H, 13C) and 2D NMR (H-H COSY, HMQC and HMBC) and mass spectra (LC-MS). Pure isolated fraction fr. 5-3 was deducted as methyl ether naragenine.
Aktivitas Sitotoksik dan Profil Kromatogram Daun Sirsak (Annona muricata L.) yang Diiradiasi ERMIN KATRIN; RHILA AMALIAH; ZUHELMI AZIZ; HENDIG WINARNO
JURNAL ILMU KEFARMASIAN INDONESIA Vol 12 No 2 (2014): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Soursop (Annona muricata L.) is one of plants included in Annonaceae which the leaves has cytotoxic activity on cancer cells. Preservation efforts of soursop leaves is carried out by gamma irradiation technic. This research aimed to study the effect of gamma irradiation on the bioactivity and chromatogram profiles of the active fraction from soursop leaves.Dry powders of soursop leaves were irradiated using gamma with doses of 5; 7.5; 10; and 15 kGy and repeated twice, respectively for certain doses. Then each sample was macerated with n-hexane, ethyl acetate and ethanol gradiently. Each extract was tested against leukemia L1210 cells. Ethyl acetate extract was the most active (IC50= 7.36 µg/mL) compared with n-hexane (20.18 µg/mL) and ethanol extract (13.89 µg/mL). Ethyl acetate extracts was fractionated using column chromatography obtained ten fractions. The result of cytotoxic activities assay of ten fractions were obtained that fraction 8 was the most active with IC50 value of 0.45 µg/mL. Cytotoxic activity of fraction 8 from irradiated samples showed that gamma irradiation decreased cytotoxic activity of fraction 8, but they were still active (IC50 < 20 µg/mL). Analysis and identification of fraction 8 by the HPLC and TLC-densitometry showed that there were broad peak which decreased in the irradiated samples.The maximum radiation dose for soursop leaves without damaging its bioactivity and that did not change the chromatogram profilesis 7.5 kGy.
Iradiasi Sediaan Obat Herbal Temu Putih Curcuma zedoaria (Berg) Rosc.: Cemaran Mikroba, Sitotoksisitas dan Profil Kromatogram ERMIN KATRIN W.; EPSI NARULITA; ZUHELMI AZIZ; HENDIG WINARNO
JURNAL ILMU KEFARMASIAN INDONESIA Vol 11 No 1 (2013): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Gamma irradiation has been used for preservation of herbal medicines, one of which is temu putih Curcuma zedoaria (Berg) Rosc. to cure cervical cancer. This research aimed to study the gamma irradiation effects on microbial contamination, cytotoxic activity against leukemia L1210 cell lines and chromatogram profile of the temu putih active fraction. Gamma irradiation was performed with a 60Co source at 5, 7.5, 10 and 15 kGy. After irradiation at > 7.5 kGy bacteria and mould were not present on herbs. Control and irradiated samples were consecutively macerated with n-hexane, ethyl acetate and ethanol. Ethyl acetate extract was the most cytotoxic gainst L1210 leukemia cells with an IC50 value of 10.60 μg/mL as compared to n-hexane or ethanol extract. Ethyl acetate extract was fractionated with column chromatography producing six fractions. Fraction 2 was the most cytotoxic against L1210 leukemia cells with an IC50 value of 2.44 μg/mL. The thin-layer chromatographic analysis results of ethyl acetate extract and fraction 2 of control and irradiated samples showed the presence of curcumin and chromatographic pattern similar to the control. Curcumin contents in fraction 2 were between 35 to 51%, which were not significantly changed between samples, although was irradiated up to 15 kGy. The maximum dose for irradiation of temu putih herbal preparation is 15kGy.
Co-Authors Anny Victor Purba EPSI NARULITA ERMIN KATRIN ERMIN KATRIN W. MUHAMMAD FITRAH Purgiyanti Purgiyanti RHILA AMALIAH Simanjuntak, Partomuan Zuhelmi Aziz