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All Journal IPTEK Journal of Proceedings Series Jurnal Matematika dan Ilmu Pengetahuan Alam Indonesian Journal of Tropical and Infectious Disease INDONESIAN JOURNAL OF PHARMACY Jurnal Kimia Riset Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Jurnal Abdi: Media Pengabdian Kepada Masyarakat Bubungan Tinggi: Jurnal Pengabdian Masyarakat

Harsasi Setyawati

Departemen Of Chemistry, Faculty Of Science Technology, Universitas Airlangga, Indonesia

Author-ID : 3195328

Humanities Chemistry Computer Science & IT Earth & Planetary Sciences Education Health Professions Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Public Health Social Sciences Other

Published : 17 Documents Claim Missing Document

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SINTESIS DAN KARAKTERISASI SENYAWA KOMPLEKS Zn(II)-EDTA SEBAGAI SENAYAWA ANTIALGA PADA COOLING WATER INDUSTRI Harsasi Setyawati; Sri Sumarsih; Sevia Ayuningtyas
Jurnal Kimia Riset Vol. 2 No. 1 (2017): Juni
Publisher : Universitas Airlangga, Campus C Mulyorejo, Surabaya, Indonesia

ABSTRACTA research on the synthesis and characterization of complex compounds of Zn (II)-EDTA as antialgae compound is applied to the cooling water industry. This research aims to determine the activity of complex compounds of Zn (II)-EDTA against algae that live in the water cooling water. The activity antialgae assay of comple compound of Zn(II)-EDTA with luminescence method and dry cell weight method. Complex compound of Zn (II)-EDTA made with mole ratio of ZnCl2: Na2EDTA is 1:1. Complex compound of Zn (II)-EDTA analyzed using UV-Vis spectrophotometer and FTIR spectrophotometer. The results of UV-Vis spectrophotometer analysis showed that the complex compounds of Zn (II)-EDTA has a maximum wavelength at 752 nm. While the results of FTIR analysis showed Zn-O vibration absorption at wave number 478.35 cm-1 and Zn-N vibration absorption at wave number 516.92 cm-1. In the activity antialgae assay of complex compound of Zn (II)-EDTA made with a concentration of 5 ppm, 10 ppm, 50 ppm and 100 ppm. The test results showed that the activity of complex compounds of Zn (II) -EDTA can kill green algae and brown algae. Of the four concentrations of complex compounds of Zn (II)-EDTA, green algae and brown algae can be killed optimally at a concentration of 50 ppm.Keywords: complex Zn(II)-EDTA, cooling water, antialgae, green algae, brown algae

The Influence of pH Values on the Crystallite Size of ZnO by Solvothermal Synthesis Ahmadi Jaya Permana; Dian Wulandari; Hartati Hartati; Harsasi Setyawati; Mochammad Zakki Fahmi
Jurnal Kimia Riset Vol. 2 No. 2 (2017): Desember
Publisher : Universitas Airlangga

Zinc oxide (ZnO) is a semiconductor material that widely used in various applications due to its unique properties. Synthesis of ZnO by solvothermal method has been conducted with controlled pH values. The variations of pH value were 10, 11 and 12 by adjusting NaOH content. Crystall structure of the synthesis products after heat treatment at 110oC and 600oC has characterised by X-ray Diffratometer (XRD). Crystallite size of ZnO was measured by Scherrer equation. Crystall phase of ZnO has been observed on all pH value variations at 110 oC with 22,98-37,06 nm of crystallite size, whereas ZnO has been observed on all pH value variations at 600 oC with 41,39-71,77 nm of crystallite size.Keywords: ZnO, pH values, crystallite size, solvothermal

RNA Isolation of Dengue Virus Type 2 with Different Precipitation Solvents : Methanol, Chloroform, and 2-Isopropanol. Yovilianda Maulitiva Untoro; Teguh Hari Sucipto; Harsasi Setyawati; Siti Churrotin; Ilham Harlan Amarullah; Puspa Wardhani; Aryati Aryati; Shuhai Ueda; Soegeng Soegijanto
Jurnal Kimia Riset Vol. 3 No. 1 (2018): Juni
Publisher : Universitas Airlangga

Dengue virus distributed in tropical and subtropical regions in the world. DENV viruses are transmitted between humans primarily by Aedes aegypti and Aedes albopictus mosquitoes and are endemic in most areas in which the vectors occur. Four serotypes of dengue virus are DENV-1, DENV-2, DENV-3 and DENV-4. DENV-2 is comprised of six genotypes. Ethanol precipitation is a commonly used technique for concentrating and de-salting nucleic acids (DNA or RNA) preparations in aqueous solution. RNA isolation by combining Guanidinium thiocyanate and phenol reported has been reported. In this report, we investigated RNA isolation from DENV-2 using QIAamp Mini Kit with 2-Isopropanol, Methanol, Chloroform precipitation solvent. Electrophoregram showed DNA band as the result of RNA isolation with methanol and 2-isopropanol are produced quite well. Dna band of the of RNA isolation with chloroform solvent has the lowest intensity than methanol and 2-isopropanol. This study showed that methanol and 2-isopropanol can used as precipitation solvent for isolating RNA.

Precipitation Solvents for RNA Extraction of Dengue Virus Type 3: Dimethylformamide, Ethylenediamintetraacetic Acid, and Ultrapure H2O Rizqidhana Juliana Putri; Teguh Hari Sucipto; Harsasi Setyawati; Siti Churrotin; Ilham Harlan Amarullah; Puspa Wardhani; Aryati Aryati; Soegeng Soegijanto
Jurnal Kimia Riset Vol. 3 No. 2 (2018): Desember
Publisher : Universitas Airlangga

Dengue is a disease caused by a virus from the family Flaviviradae, carried by a female mosquito of Aedes aegypti species. Dengue fever is widespread in the tropic areas. It caused by rainfall, temperature and unplanned urbanization. According to the ministry of health , almost all provinces in Indonesia are endemic areas of dengue fever. In 2014, up to mid-December Dengue Hemorrhagic Fever (DHF) patients in 34 provinces in Indonesia are 71,668 people and 641. This figure is lower than the previous year, 2013 with 112,511 people and 871 deaths . This disease consists of four types of serotypes, namely DENV-1, DENV-2, DENV-3, and DENV-4. This disease can be identified using a variety of methods, one of the method is Reverse Transcription - Polymerase Chain Reaction (RT-PCR) method. This study aims to determine the ability of Dimethylformamide (DMF), Ethylenediamintetraacetic Acid (EDTA), and Ultrapure H2O as the substitute of Ethanol for precipitation in RNA extraction process. The sample used in this research obtained from Surabaya. RNA extraction itself can be done by using a special kit for RNA extraction. In Reverse Transcription - Polymerase Chain Reaction method, first RNA is extracted and then transcribed back (Reverse Transcription) which then form cDNA that later will be amplified by using PCR method. In this study used specific primers for dengue virus type 3 (DENV-3). The results of this study show that DMF, EDTA, and Ultrapure H2O can be used as the substitute of Ethanol for precipitation on RNA extraction. The result is evidenced by the formation of viral DNA bands on gel electrophoresis results.

SINTESIS, KARAKTERISASI DAN UJI AKTIVITAS SENYAWA KOMPLEKS Zn(II)-KATEKIN SEBAGAI INHIBITOR ENZIM LIPASE Antyka Lutfiana Putri; Sri Sumarsih; Harsasi Setyawati
Jurnal Kimia Riset Vol. 4 No. 1 (2019): Juni
Publisher : Universitas Airlangga

ABSTRACT The study aims to synthesis, characterization and activity testing of Zn(II)-catechin as lipase inhibitor activity. The complex compound from synthesis result is characterized by its maximum wavelength, functional groups, metals and ligands bonds, and it’s melting point. This complex compound tested of Zn(II)-catechin as lipase inhibitor activity to p-NPP (para-nitrofenilpalmitat) substrate. The result shows that Zn(II)-catechin could be synthesized from Zn(II) ligans and metals with ratio ligands and metals mol is 1:1, that has maximum wavelength 454 nm, which contain Zn-O bond at 354,90 and 478,35 cm-1, and melting point of Zn(II)-catechin >250 oC. Zn(II)-catechin can inhibition 60,63% from concentration 50 µg/mL with mixed inhibition Keyword : Zn(II)-catechin, lipase, mixed inhibition ABSTRAK Penelitian ini bertujuan untuk melakukan sintesis, karakterisasi dan uji aktivitas senyawa kompleks Zn(II)-katekin terhadap aktivitas inhibitor enzim lipase. Senyawa kompleks hasil sintesis dikarakterisasi panjang gelombang maksimumnya, gugus fungsi serta ikatan logam dan ligannya, serta titik lelehnya. Senyawa kompleks diuji aktivitas inhibitor enzim lipase terhadap substrat p-NPP (paranitrofenilpalmitat). Hasil penelitian menunjukkan bahwa senyawa kompleks Zn(II)-katekin dapat disintesis dari ligan katekin dan logam Zn(II) dengan perbandingan mol ligan logam 1:1, mempunyai panjang gelombang maksimum 454 nm, mengandung ikatan Zn-O pada 354,90 dan 478,35 cm-1 dan titik leleh senyawa kompleks >250 oC. Senyawa kompleks Zn(II)-katekin pada konsentrasi 50µg/mL mempunyai daya inhinbisi sebesar 60,63% terhadap enzim lipase dengan jenis inhibisi campuran. Kata kunci : Zn(II)-katekin, lipase, inhibitor campuran

Co-Authors A. Budi Prasetyo Ahlan Riwahyu Habibi Ahmadi Jaya Permana Aisyah, Nourmalasari Alfa Akustia Widati Aning Purwaningsih Anisa Maharani Antyka Lutfiana Putri Aryati Aryati Atik Widiyanti Aulianitha Salsabella DIAN WULANDARI Fahimah Martak Fatiha Khairunnisa Handoko Darmokoesoemo Hartati Hartati Hartati Hartati Ilham Harlan Amarullah Kariza Makanty Kautsar Ul Haq Kris Cahyo Mulyatno, Kris Cahyo Kris Murwani, Irmina Maharani, Anisa Masanori Kameoka, Masanori Miratul Khasanah Mochammad Zakki Fahmi Mulyadi Tanjung Novia Faridatus Sholihah PUJI LESTARI Purkan Purkan Puspa Wardhani Qurrota A’yuni Rico Ramadhan, Rico Rizqidhana Juliana Putri Sevia Ayuningtyas Shuhai Ueda Siti Churrotin, Siti Siti Wafiroh Soegeng Soegijanto Sofijan Hadi Sri Subekti Sri Sumarsih Syafsir Akhlus Teguh Hari Sucipto Teguh Hari Sucipto, Teguh Hari Tokok Ardiarto Tomohiro Kotaki, Tomohiro Ueda, Shuhai Wardhani, Puspa Wibrianto, Aswandi Yanuardi Raharjo, Yanuardi Yovilianda Maulitiva Untoro

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