Widya Saraswati
Department Of Conservative Dentistry, Faculty Of Dental Medicine, Universitas Airlangga

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Journal : Conservative Dentistry Journal

The inhibitory efficacy of flavonoid of mangosteen peel extract (Garcinia Mangostana Linn.) against Lactobacillus Acidophilus biofilm bacteria Dyah Utari Wahyu Ningrum; Karlina Samadi; Widya Saraswati
Conservative Dentistry Journal Vol. 10 No. 2 (2020): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v10i2.2020.75-79

Abstract

Background: Lactobacillus takes role in the formation of dental plaque which is the main factor that cause dental caries. The bacteria take role in the metabolism of glucose in the mouth, producing organic acids which lower the pH in the oral cavity. This situation can lead to the formation of dental caries because these bacteria can form biofilm as a defense of microorganisms to antibiotics and the immune response. Mangosteen peel has active ingredients such as flavonoid that can be used to inhibit biofilm. The ability of flavonoid compounds in the phenol group can make the bacterial enzyme becomes inactive, causing the activity of glucosyltransferase enzyme that usually used by bacteria to synthesize sucrose in the medium becomes glucan. As a result, bacterial biofilm formation is inhibited because the amount of glucan as a medium of bacterial attachment is limited, but until now the effectivity dose of flavonoid from mangosteen peel extract (Garcinia mangostana Linn.) is has not known yet Purpose: The aim of this study was to determine the inhibitory efficacy of flavonoid of mangosteen peel extract against the formation of Lactobacillus acidophilus bacterial biofilm. Methods: Bacteria that had been formed into biofilms was studied in two times treatment, one concentration reviewed by flavonoid from mangosteen peel extract and without reviewed by flavonoid from mangosteen peel extract. The treatment group was incubated at 37°C until day 8 since the first day incubated. The treatment group was washed with phosphate buffered saline (PBS) 4 times and dried. The treatment group stained with 0.2 ml 0.1% crystal violet and 15 min incubation. The treatment group was rinsed with distilled water 3 times and then dried. Solvent DMSO 100% was added as much as 0.1 ml in each well. Microtiter plate was shaken for 1 minute and then placed in to the microplate reader and then the OD (Optical Density) can be read. Results: There were significant differences between the inhibitory efficacy of flavonoid of mangosteen peel extract against Lactobacillus acidophilus biofilm bacteria, the control group and group treatments (p <0.05). Conclusion: Flavonoid of mangosteen peel extract with the concentration of 0.78% had inhibitory effect against Lactobacillus acidophilus biofilm bacteria
The Role of Propolis in Pulp Pain by Inhibiting Cyclooxygenase-2 Expression Ira Widjiastuti; Widya Saraswati; Annisa Rahma
Conservative Dentistry Journal Vol. 11 No. 1 (2021): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v11i1.2021.11-18

Abstract

Background: Inflammation of the pulp can lead to elicit pain. Pain in inflammation is induced by the cyclooxygenase-2 enzyme (COX-2) which induces prostaglandin E2 (PGE2) resulting in pain. Pain in the pulp can be relieved by eugenol. In its application, eugenol is toxic to pulp fibroblasts. Due to the side effect, it is worth considering other biocompatible materials with minimal side effects, such as propolis. Flavonoids and phenolic acids that contained in propolis can inhibit COX-2. Therefore, an analysis outlined in the literature review is needed to examine the results of research related to the role of propolis as pulp pain relief by inhibiting COX-2 expression. Purpose: To analyze the role of propolis in pulp pain by inhibiting COX-2 expression. Reviews: Propolis extract that extracted by ethanol, water, and hydroalcohol has pain relief properties in the pulp by inhibiting COX-2 by directly binding to the COX-2 receptors and by reducing the production of proinflammatory cytokines which are COX-2 inducers, proven through in vivo, in vitro, and in silico studies in various target cell organs. Conclusion: Propolis extract has high prospect as inflammatory pain inhibitor in the pulp by inhibit COX-2 expression.
Comparison Adhesive strength of Resin Composite between Total-etch and Self-etch Techniques on Enamel After Fluoride Application Widya Saraswati; Adioro Soetojo; Yasmin Tasya Brilyanti
Conservative Dentistry Journal Vol. 11 No. 1 (2021): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v11i1.2021.38-41

Abstract

Background : White Spot Lesion (WSL) is caused by Streptococcus Mutan which causes demineralization of teeth. One of the treatments of WSL is topical application of fluoride which aims to remineralize the teeth. However, if topical fluoride treatment does not give satisfactory results, alternative treatments can be made using restorative treatments, one of which is veneers. The procedure for applying veneers requires the application of an adhesive system. The adhesive system used to apply veneers is self-etch and total-etch. Several references state that the application of fluoride which aims to remineralize teeth can influence the adhesiv strength of the restorative material using self etch and total-etch techniques. Purpose : To determine the effect of composite resin adhesion strength between total-etch and self etch techniques on enamel after fluoride application. Review : Literature sources used in the preparation of the article through several databases with descriptions of the effects of fluoride application before total-etch and/or self etch administration. From the existing references, it was found that the application of fluoride before the total-etch system had a less significant effect on the adhesiv strength. Whereas in the self etch system, fluoride application has a significant effect on the adhesiv strength. Conclusion : More references say that the application of fluoride before the total-etch system has a better adhesive strength than the application of fluoride before the self etch system
White pomegranate (Punica granatum) peels extract bactericidal potency on Enterococcus faecalis Eric Priyo Prasetyo; Widya Saraswati; Dian Agustin Wahjuningrum; Latief Mooduto; Rizka Firdaus Rosidin; Evelyn Tjendronegoro
Conservative Dentistry Journal Vol. 11 No. 2 (2021): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v11i2.2021.84-88

Abstract

Background: Enterococcus faecalis (E. faecalis) is the most resistant bacteria in the root canals and one of the causes of recurrent endodontic treatment. Enterococcus faecalis was not the only bacteria found in failed endodontic treatment but these bacteria can thrive in unfavorable environment. White pomegranate (Punica granatum) is known for its flavonoids and tannins that function as antimicrobial agent. White pomegranate extract is potential for use as disinfection or irrigation material. Purpose: This aim of this study was to determine the bactericidal potency of white pomegranate (Punica granatum) extract on E. faecalis growth. Methods: This study was an in vitro experimental observation. E. faecalis was obtained from stock culture taken from the root canal of recurrent endodontic treatment. E. faecalis from the serial dilution were cultured in blood agar media. Antibacterial potency was determined by colony calculation of E. faecalis growth in blood agar in colony forming unit (CFU) and conducted in 6 replications for each concentration group. Statistical analysis was done using one-way analysis of variance at 5% significance level. Results: White pomegranate peels extract concentrations of 3.125%, 6.25%, 12.5% and 25% provide significant decrease in the number of E. faecalis colony compared to the control group (p<0.05). No bacterial growth was found on 25%, 50% and 100% concentration. Conclusion: The potent minimal bactericidal concentration of white pomegranate peels extract on E. faecalis was 25%.
Microleakage Difference of Self-Adhering Flowable Composite and Flowable Composite After Sitric Acid Immersion Widya saraswati; Adioro Soetojo; Veda Sahasika Azaria Nariswari; Annisa Salsabila Andika Putri; Sekar Firdhea Rizkifa Soetanto
Conservative Dentistry Journal Vol. 11 No. 2 (2021): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v11i2.2021.89-92

Abstract

Background: Flowable composite (FC) is a composite that has low viscosity so that it can flow to the cavity. Selfadhering flowable composite (SAC) is a type of flowable composite which is applied without the stages of etching and bonding because it has an acid monomer. Degradation in the oral cavity such as exposure to acidic fluid and changes in temperature can result in microleakage between the restoration and the cavity wall which can cause secondary caries, and hypersensitivity. Purpose: To prove the difference of microleakage between self-adhering flowable composite (SAC) and flowable composite (FC) after immersed in citric acid. Methods: 28 bovine incisors were cleaned and soaked with 0.01% thymol then divided randomly into 4 groups, groups 1 and 2 are immersed in citric acid with SAC restoration and FC restoration. Groups 3 and 4 are SAC and FC material control groups. All groups were prepared class V with cylindrical shapes. Groups 1 and 2 were treated with thermocycling from 5o and 55o C for 120 cycles and immersed in 3364 ppm citric acid for 24 hours. All groups were immersed in 2% methylene blue for 24 hours, then buccolingual cut for 1 mm. Evaluation of microleakage was seen by the amount of color that entered between the restoration wall and the cavity using a 40x magnification Digital Microscope. Test data analysis using the Kruskal Wallis and Mann Whitney U. Results: Significant differences were found between SAC and FC. Significant differences were also obtained from SAC compared with the SAC control group. Whereas in the comparison between FC and FC control group no differences were found. Conclusion: Microleakage SAC is bigger than FC and SAC control group, but FC has no difference with FC control group.
Anterior maxillary teeth crown lenghtening and indirect veneer for esthetics rehabilitation: A case report Ganiezha Cindananti; Nina Dhaniar; Nurshiffa Hanifatul Amrina Jaelani; Cathelea Raihan Avicenna; Widya Saraswati
Conservative Dentistry Journal Vol. 12 No. 1 (2022): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v12i1.2022.20-25

Abstract

Background: Crown Lengthening is a surgical procedure performed to create a symmetrical gingival line and is carried out to expose necessary additional tooth structure. A short clinical crown occurs as a result of dental malformations, eruption disharmony, and genetic variation. Purpose: The purpose of this case report is to explain the treatment management of Crown Lengthening over the case of diastema closure by indirect veneer restoration. Case: 32 years old female presented with a chief complaint over a space between her anterior maxillary teeth. She wants to rectify her smile. Case Management: The management in this case are preparation of Digital Smile Design (DSD), Crown Lengthening on 11 and 21, and Indirect Veneer on 11, 21, 22. Conclusion: Aesthetical rehabilitation could be attained by an indirect veneer treatment followed by a gingival management which is Crown Lengthening.
Compressive Strength Of Fiber Reinforced Composite After Immersion In Citric Acid Of Energy Drink Agnes Robia Ambarioni; Alfina Putri Nurrahmania; Chaerun Mutmainnah; Widya Saraswati; Cecilia G.J.Lunardhi
Conservative Dentistry Journal Vol. 12 No. 1 (2022): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v12i1.2022.45-48

Abstract

Background: Energy drink consumption has been popular in people age 18-35 years old. A few literature showed that this drink can cause damage to teeth and composite due to its acidity. The use of fiber reinforced composite has been increasing due to its good mechanical properties and aesthetic. Fiber reinforced composite has fiber shaped fillers that can withstand mastication forces. A restoration material has to face the complex oral environment to succeed clinically. Compressive strength test is needed to predict the durability of restoration materials against mastication forces. Purpose: To determine the effect of energy drink citric acid immersion to fiber reinforced composite compressive strength. Methods: 32 cylinder shaped fiber reinforced composite samples with 8mm tall and 4mm diameter were divided into two groups. The first group was immerse in distilled water (K1) as control, and the second group (K2) was immerse in energy drink citric acid. The samples were immersed in an incubator at ±37⁰C for 7 days. After 7 days, samples were washed in water, dried with tissue, and then undergo compressive strength test with Autograph machine at crosshead speed of 10mm/minute. The data were then analyzed using Independent t-test. Results: The mean compressive strength of K1 group was 1469.63 MPa and K2 group 1439.57 MPa. The mean value of K2 group was slightly lower than K1 group, but statistically, there was no significant difference between the 2 groups. Conclusion: Energy drink citric acid immersion has no effect on fiber reinforced composite compressive strength.
Single visit root canal treatment with post crown restoration Ismiyatin, Kun; Saraswati, Widya; Cahyani, Febriastuti; Vivian Widjaya, Olivia; Ghozali Pangestu, Dede; Arshila, Dea
Conservative Dentistry Journal Vol. 12 No. 2 (2022): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v12i2.2022.57-62

Abstract

Background: Single visit endodontics has been shown to be an effective treatment modality when compared with multiple visit therapy and it does not deviate from achieving the objectives of proper biomechanical preparation, debridement, shaping, disinfection and 3 dimensional obturation of root canal system and is more beneficial to the patient and dentist provided there is careful case selection and strict follow of standard endodontic protocols. Symptomatic teeth can also be managed by single visit after controlling the abscess infection with antibiotic, provided the root canal is dry without any discharge.Purpose: This study aims to perform single visit root canals on teeth with irreversible pulpitis and restoration with post core and crown. Case(s): A 48-years-old female comes with dental complaints of upper left rear spontaneous pain at night. The tooth has had cavities since about two years ago and has never been previously treated. The patient said had not any systematic health disorder. There is a spontaneous pain that the patient feels. Case Management: From the examination that has been carried out, a clinical diagnosis of tooth 25 were symptomatic pulpitis irreversible with normal apical tissue. The restoration endodontic treatment plan was a single visit endodontic and the restoration is post core and crown. Conclusion: Symptomatic pulpitis irreversible with normal apical tissue treatment can be carried out single visit root canal treatment. Restoration of post endodontic treatment in this case need fiber post and core using composite and finally restoration using crown that made of zirconia. Zirconia has a higher level of material resistance than lithium disilicate. This is what makes zirconia the choice, especially for posterior teeth.
One Visit Endodontic with Internal Bleaching on Tooth 12 Post Trauma: A Case Report Pasenda Mulyadi, Reyz; Arnesti Wahab, Sofi; Cahyani, Febriastuti; Saraswati, Widya
Conservative Dentistry Journal Vol. 12 No. 2 (2022): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v12i2.2022.63-67

Abstract

Introduction: Post-traumatic non-vital teeth is one of the indications for One Visit Endodontic. Trauma to the teeth may arise from conditions that cause the teeth to become necrotic so that the teeth will look darker and experience discoloration. Clinical conditions that indicate the death of the dental pulp and teeth require endodontic treatment. This situation will cause discoloration of the teeth that occur as a result of the metamorphosis of calcified pulp. Internal whitening is a treatment that can be done after the first treatment. Purpose: To provide information about discolored teeth management after trauma which causes pulp necrosis by doing root canal treatment and internal bleaching to change tooth color. Case: A 31-year-old woman came to the RSKGMP Universitas Airlangga with a referral from the orthodontics department where her teeth were darker in color and interfered with her appearance. In the previous history, the patient had an accident and trauma 10 years ago. Case Management: The first step is access opening and a good glide path. Root canal preparation was performed using the Crown Down Pressureless technique. The obturation technique used is Single Cone. The results were good and there were no symptoms during the control. Conclusion: One visit endodontic can be a treatment option for post-traumatic non-vital teeth. Internal bleaching with Walking Bleach Technique is also proven effective in restoring the original tooth color without excessive reduction of the dental crown.
The Effect of Adding Epigallocatechin-3-gallate on the Porosity of Calcium Hydroxide Ismiyatin, Kun; Saraswati, Widya; Sudarsono, Caeleb Fabrizio
Conservative Dentistry Journal Vol. 13 No. 1 (2023): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v13i1.2023.11-15

Abstract

Background: Dental caries is a very common non-communicablae disease found in all age groups and found in 3.5 billion people in the world. Caries that harm the pulp tissue can be treated by direct or indirect pulp capping. One of the physical properties possessed by pulp capping is the porosity of material. The material most often used for pulp capping treatment of pulp perforations is calcium hydroxide (Ca(OH) 2 ). However, Ca(OH) 2 has a high pH that caused the porosity to be low. High porosity can facilitate cell development and ion exchange. Thus, additional materials with a lower pH are needed to increase porosity such as Epigallocatechin-3-gallate (EGCG). However, the porosity of the combination of EGCG with Ca(OH) 2 is still unclear, so it needs to be researched. Purpose: To explain the differences of Ca(OH) 2 porosity after the addition of EGCG. Methods: This study used a laboratory experimental study with a post test only control group design. This research using 16 samples for each control (Ca(OH) 2 – aquadest) and treatment (Ca(OH) 2 –EGCG) group. Samples from each group were subjected to freeze drying, observed with SEM, analyzed with ImageJ software, and tested with independent T-test. Results: There was a significant difference (p&lt;0.05) in the percentage of porosity between the addition of EGCG to Ca(OH) 2 namely 32,61% compared to Ca(OH) 2 with aquadest namely 23,38%. Conclusion: The porosity of the combination of calcium hydroxide with EGCG has a higher percentage compared to calcium hydroxide with aquadest.
Co-Authors Adioro Soetojo Agnes Robia Ambarioni Ainin Nafilatus Alfina Putri Nurrahmania Alvita Wibowo Ambarwati, Venny Lusanda Anggi Puspitasari Anin Dita Annisa Rahma Annisa Salsabila Andika Putri Anuj Bhardwaj Arnesti Wahab, Sofi Arshila, Dea Auryn Thania Song Hadinata Avicenna, Cathelea Raihan Cathelea Raihan Avicenna Cecilia G.J.Lunardhi Chaerun Mutmainnah Cinitra Anindya Daradjat, Kinanti Naianuha Devi Eka Juniarti Dewi Kusuma Wardani Dian Agustin Wahjuningrum Dian Pramita Ayu Kumalasari Dur Muhammad Lashari Dyah Utari Wahyu Ningrum Dzulfikri, Ahzahra Indah Ekawati Ekawati Eric Priyo Prasetyo Erin Imaniar B Evelyn Tjendronegoro Febriastuti Cahyani Gabriela Kevina Alifen Galih Sampoerno Ganiezha Cindananti Ghozali Pangestu, Dede Goenharto, Setyabudi Gusniarti, Haninda Dhipta Halima, Siti Noorraida Ida Ayu Putu Sri Widnyani Ira Widjiastuti Jaelani, Nurshiffa Hanifatul Amrina Karlina Samadi Ketut Suardita, Ketut Kun Ismiyatin Latief Mooduto, Latief Leidy Herlin Rumbiak Lestari, Vita M. Mudjiono Masa, Maria Margaretha S. Nogo Mulad, Iga Arziela Mulyadi, Reyz Pasenda Mutmainnah, Chaerun Nadhifa Salma Namira, Safa Marety Nanik Zubaidah Nina Dhaniar Nirawati Pribadi Nurshiffa Hanifatul Amrina Jaelani Oktaria, Wanda Pasenda Mulyadi, Reyz Pratiwi, Dian Dwi Rahardia, Nabiela Ramadani, Rania Rizka Riyadi, Tiara Lathifah Rizka Firdaus Rosidin Ruslan Effendy Ruslan Effendy Saravanan, Pravinna Sekar Firdhea Rizkifa Soetanto Setyabudi Sri Kunarti Sri Kunarti, Sri Sudarsono, Caeleb Fabrizio Sukaton, Sukaton Sutela, I Gede Marantika Yogananda Tanzil, Muhammad Iqbal Tasya Nafatila Veda Sahasika Azaria Nariswari Vina Zavira Nizar Vita Ariesta Vivian Widjaya, Olivia Wahab, Sofi Arnesti Widjaja, Olivia Vivian Wulandini, Raden Ajeng Yasmin Tasya Brilyanti Yonas, Yovita Yovita Yonas