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All Journal AGRIKA Naturalis : Jurnal Penelitian Pengelolaan Sumberdaya Alam dan Lingkungan Jurnal Ilmu-Ilmu Pertanian Indonesia Jurnal Agrotek Lestari Jurnal Agrium Jurnal Ilmu Pertanian Tirtayasa Jurnal Penelitian Agrosamudra Jurnal Masyarakat Madani Indonesia Eumpang Breuh: Jurnal Pengabdian Masyarakat
Boy Riza Juanda
Dosen Program Studi Agroteknologi, Fakultas Pertanian, Universitas Samudra, Langsa
Religion Agriculture, Biological Sciences & Forestry Humanities Computer Science & IT Earth & Planetary Sciences Education Public Health Social Sciences Other

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Eksplorasi Bakteri Endofit Terhadap Cendawan Pestalotiopsis microspora Penyebab Penyakit Gugur Daun Pada Tanaman Karet (Hevea brasiliensis Muell. Arg.) Vazza Navtra Tylova; Syamsul Bahri; Boy Riza Juanda; Alchemi Putri Juliantika Kusdiana
Jurnal Ilmu-Ilmu Pertanian Indonesia Vol 25 No 1 (2023)
Publisher : BPFP Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jipi.25.1.51-58

Abstract

[The Potential of Endophytic Bacteria as a Biological Controller of Leaf Fall Disease Caused by Pestalotiopsis sp. IN Rubber Plants (Hevea brasiliensis Muell. Arg.]. This study aims to explore endophytic bacteria and determine their effectiveness against the fungus Pestalotiopsis sp. which causes leaf fall on rubber plants. Pestalotiopsis sp. can attack all rubber clones resulting in decreased latex production. The presence of pathogen attacks can result in enormous economic losses. Therefore, it is very important to find an effective and efficient controlling method, one of which is through biological control of plant diseases, namely by using antagonistic microorganisms. The presence of endophytic bacteria as biological controllers has become an alternative method to reduce the practices of chemical control. This study used a Non-Factorial Completely Randomized Design (CRD) with 6 treatments and 4 replications, so that 24 experimental units were obtained, using Potato Dextrose Agar (PDA) media for the in vitro antagonist test using the dual culture method. Namely P₀ = Control, P₁ = Pestalotiopsis sp. 1 vs Bacteria P, P₂ = Pestalotiopsis sp. 2 vs Bacteria B, P₃ = Pestalotiopsis sp. 3 vs Bacteria C, P₄ = Pestalotiopsis sp. 4 vs Bacteria E, P₅ = Pestalotiopsis sp. 5 vs Bacteria F. The results indicated that antagonistic treatment of endophytic bacteria in vitro affected the percentage of inhibitory power of the mycelium of the fungus Pestalotiopsis sp. by endophytic bacteria at 1 - 6 Days After Incubation (DAI). The 3% KOH method and gram staining showed that samples P₁, P₂, P₅ did not produce mucus and were purple in colour (positive), but samples P₃, P₄, showed mucus and were pink in colour (negative). Hypersensitivity reactions to tobacco plants samples P₁, P₂, P₃, P₄, P₅ did not show negative characteristics or did not cause changes in coloration and symptoms on leaves of tobacco plant.  
Co-Authors Adnan Adnan Adnan Amin Al Mustari Alchemi Putri Juliantika Kusdiana Andi Syahputra Perdana Asmaul Husna, Asna Dewi Hasibuan Basriwijaya, Kiagus Muhammad Zain Cut Gustiana Cut Mulyani Dea Musvita dea Desi Asnita Diana Safitri Eko Cahyo Pristiwantoro Fajar Pratama Farasmayuda Hanisah Hanisah Hanisah Husna Maulida Iswahyudi, Iswahyudi Karnelis Karnelis Kiagus M Zain Basriwijaya Liza Agnesta Krista M Syahril Muhammad Haiqal Muhammad Rian Nugraha Muhammad Zaini Putri Nur Elvadita, Reza Tanjung Rezky Apriani Risky Ridha Risky Ridha Romaito Simatupang Rosmaiti, Rosmaiti Rozalina Rozalina Saputra, Iwan Silvia Anzitha Siregar, Dolly Sojuangan sofiyana sofiyana Supriyadi Syamsul Bahri Syukri Syukri Risyad Vazza Navtra Tylova Zulhilmi Zulhilmi