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Journal : Jurnal Sain Veteriner

Penilaian Organisasi Perangkat Daerah (OPD) tentang Otoritas Veteriner dalam Kebijakan Penanggulangan Rabies di Provinsi Banten Ismadi, Joko; Sumiarto, Bambang; Nugroho, Widagdo Sri; Purwanto, Erwan Agus
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (797.726 KB) | DOI: 10.22146/jsv.57366

Abstract

Veterinary Authority (Otovet) is a government institution, formed in order to deal with decision making of anything related to animal health, by involving veterinarians and mobilizing all related professional lines. The purpose of this study was to describe the Regional Organizations’ (OPD) assessment of the implementation of Veterinary Authority (Otovet) in rabies management policies. Research material included 36 Regional organizations (OPD), 9 Technical services, supervising animal health (keswan) and veterinary public health (kesmavet), 9 Health services, 9 Regional development planning services, 9 Regional staffing services, Banten Province. The results comprised eight questions: 1). Are the organization bureaucratic? 2). Positions, roles, and authority of veterinarians? 3). Is the veterinary authority regarding rabies control already in line with the implementation? 4). Strategic policy 5). Cooperations OPD, 6). Regional Policy 7). Veterinarian openings, and  8). Enforcement and improvement of otovet institutions. The answers were: Technical services of animal health and veterinary public health: 0%, 33,33%, 77,78%, 100%, 33,33%, 44,44%, 77,78%, 100% answered yes; 100%, 66,67%, 22,22%, 0%, 66,67%, 55,56%, 22,22%, 0% answered no. Health services: 22,22%, 66,67%, 88,89%, 44,44%, 55,56%, 22,22%, 0% , 77,78% answered yes; 77,78%, 33,33%, 11,11%, 55,56%, 44,44%, 77,78%, 100%, 22,22% answered no. Regional development planning services, 33,33%, 88,89%, 66,67%, 11,11%, 33,33%, 33,33%, 33,33%, 100% answered yes; 66,67%, 11,11%, 33,33%, 88,89%, 66,67%, 66,67%, 66,67%, 0% answered no; Regional staffing services 88,89%, 77,78%, 55,56%, 0%, 11,11%, 11,11%, 100%, 66,67% answered yes; 11,11%, 22,22%, 44,44%, 100%, 88,89%, 88,89%, 0%, !00%, answered no. In conclusion, based on the quantitative data collected, the Regional Organizations’ (OPD) assessment of the implementation of Veterinary Authority (Otovet) in rabies management policies were as follows:  4 OPDs scored well and 5 OPDs scored poor. Health services: 4 OPDs scored well and 5 OPDs scored poor. Regional development planning services: 4 OPDs scored well and 5 OPDs scored poor. Regional staffing services: 1 scored well and 8 scored poor.
Uji Sensitivitas Bakteri Escherichia Coli Isolat Asal Ayam Yang Bereaksi Widagdo Sri Nugroho; M. Haryadi Wibowo
Jurnal Sain Veteriner Vol 23, No 1 (2005): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2825.683 KB) | DOI: 10.22146/jsv.364

Abstract

Penelitian ini bertujuan untuk mengetahui resistensi bakteri E.coli isolat ayam yang bereaksi positif pada media congo red terhadap preparat ampisilin, streptomisin, dan enrofloksasin. Delapan biak murni bakteri E.coli yang diisolasi dari kasus kolibasilosis ayam diuji afinitasnya terhadap zat warna merah kongo. Bakteri yang bereaksi positif pada media congo red tersebut, kemudian diuji sensitivitasnya menggunakan media agar Mueller-Hinton, metode disk difusi menurut Kirby-Bouer. Disk antibiotika ampisilin, streptomisin, dan enrofloksasin diposisikan menggunakan dropper pada permukaan kultur bakteri tersebut, kemudian diinkubasi selama 24 jam pada suhu 37°C. Diameter zona terang disekililing disk antibiotik yang terbentuk, kemudian diukur dalam satuan milimeter. Hasil pengukuran tersebut dibandingkan dengan referensi dan atau menurut standar interpretasi Kirby-Bouer. Hasil penelitian menunjukkan bahwa, seluruh bakteri E. coli isolat asal ayam, mempunyai penampilan penotif mengilcat zat warna merah kongo , ternyata 100 % resisten terhadap preparat ampisilin, streptomisin dan enrofloksasin.
PATOGENISITAS ISOLAT Escherichia con POSITIF CONGO RED PADA TELUR AYAM BEREMBRIO UMUR 12 HARI = PATOGENICITY OF CONGO RED POSITIVE ISOLATE OF Escherichia coil IN THE 12-DAYS OLD CHICKEN EMBRYOS Widagdo Sri Nugroho; M. Haryadi Wibowo; Widya Asmara
Jurnal Sain Veteriner Vol 20, No 1 (2002): Juli
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5076.058 KB) | DOI: 10.22146/jsv.393

Abstract

Penelitian ini bertujuan untuk mengetahui patogenisitas isolat Escherichia coif positif congo red. Escherichia coil dari kasus kolibasilosis ayam diisolasi menggunakan media TSA dan EMB kemudian kemampuan pengikatan warna congo red diuji dengan agar congo red (TSA+0,003% congo red). Tingkat patogenisitas isolat yang mengikat warns congo red dilihat dari uji kematian embrio. Empat isolat E.coli positif conga red (CR+) dan 1 isolat negatif terhadap congo red (CR -) diinokulasikan pada telur berembrio umur 12 hari. Tingkat kematian embrio selama arum bad pascainokulasi masing-masing isolat E.coli positif congo red (500 colony form unit / CM) pada kantung alantois telur berembrio umur 12 hari berbeda antar kelompok. Angka kematian yang diperoleh dari isolat CR + 1, 2, 3, dan 4 masing-masing adalah 10%, 20%, 60% dan 100%. Perubahan anatomi yang tampak yaitu terjadinya perdarahan kulit pada embrio yang mati dan secara mikroskopik lesi-lesi pada hati, jantung, dan limpa menujukkan adanya septisemi. Isolasi dan uji ulang congo red terhadap inokulat positif conga red memperlihatkan bahwa beberapa isolat kehilangan kemampuan mengikat warna conga red. Isolat-isolat tersebut memiliki angka kematian yang rendah (10-20%). Variasi kemampuan isolat mengikat warm conga red memiliki keterkaitan dengan patogenisitasnya.
Kemampuan Primer IS900 Dan F57 Mendeteksi Mycobacterium avium Subspesies Paratuberculosis Dengan PCR Konvensional Widagdo Sri Nugroho; Mirnawati Sudarwanto; Denny Widaya Lukman; Surachmi Setyaningsih; Abdulwahed Ahmed Hassan; Ewald Usleber
Jurnal Sain Veteriner Vol 26, No 1 (2008): JUNI
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.413

Abstract

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Kajian Lintas Seksional Infeksi Salmonela pada ayam Ras Petelur Berdasarkan Uji Ulas Kloaka di Kabupaten Sleman Yogyakarta Widagdo Sri Nugroho; Setyawan Budiharta; Doddy Yudhabuntara
Jurnal Sain Veteriner Vol 22, No 2 (2004): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2022.529 KB) | DOI: 10.22146/jsv.474

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Survei Infeksi Salmonella spp. pada Pasien Anjing dan Kucing di Klinik/Rumah Sakit Hewan Daerah Istimewa Yogyakarta Kajang, Elphan Augusta; Nugroho, Widagdo Sri; Haryanto, Aris
Jurnal Sain Veteriner Vol 42, No 2 (2024): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.86438

Abstract

Salmonellosis is an infectious disease that is zoonotic and includes food borne disease. Salmonella spp. can also be found in pets so that it can be a source of transmission of Salmonellosis to humans. The purpose of this study was to determine the infection level of Salmonella spp. in dogs and cats in clinics/animal hospitals in the Special Region of Yogyakarta. A total of 250 rectal swabs from 83 dogs and 167 cats were taken in this study. Isolation and identification of Salmonella spp. using Xylose Lysine Deoxycholate (XLD), Triple Sugar Iron Agar (TSIA), and Lysine Iron Agar (LIA) media. The isolates were confirmed by Polymerase Chain Reaction (PCR)using a primer Forward primer (5'- GCT AAG TAT GAC ATT CCG GT -3') and reverse (5'- CCA AAG ACT ATC TGC GGA AT -3') eith targeting the STM2773 (IroB) gene. Information on the patient's medical history was obtained based on the anamnesis and questionnaire to the animal owner. Data analysis was descriptive statistics. A total of 32 isolates of Salmomella spp. obtained through conventional methods and as many as 30 samples (12%) confirmed Salmonella spp. using PCR. A total of 11 (13.25%) of 83 dogs and 19 (11.27%) of 167 cats were identified as positive for Salmonella spp. The results of this study indicated that 12% of pet animals in the Special Region of Yogyakarta were infected with Salmonella spp.