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All Journal Jurnal Teknologi Dan Industri Pangan ACTA VETERINARIA INDONESIANA Hemera Zoa Forum Pasca Sarjana Jurnal Sain Veteriner Jurnal Veteriner Disease Prevention and Public Health Journal Jurnal Ilmu Ternak Veteriner JURNAL KAJIAN VETERINER Jurnal Agroekoteknologi dan Agribisnis Berita Kedokteran Masyarakat Jurnal Kedokteran Hewan The International Journal of Tropical Veterinary and Biomedical Research
Widagdo Sri Nugroho
Departemen Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan, Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Nursing Public Health Veterinary

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Journal : Jurnal Ilmu Ternak Veteriner

 1 
Detection of Mycobacterium avium subspecies paratuberculosis of dairy cows in Bogor Nugroho, Widagdo Sri; Sudarwanto, M.; Lukman, D.W; Setiyaningsih, E.; Usleber, E.
Indonesian Journal of Animal and Veterinary Sciences Vol 14, No 4 (2009)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (304.452 KB) | DOI: 10.14334/jitv.v14i4.335

Abstract

Johne’s disease (JD) or partuberculosis is a chronic granulomatous enteritis in ruminants caused by infection of Mycobacterium avium paratuberculosis subspecies (MAP). The disease has been detected serologically in Indonesia. It’s potential to spread to other herds and could create great economic losses. The objectives of current study were to detect MAP in milk and faeces of dairy cows as well as to evaluate the association between farm management factors and presence of the bacteria in dairy cows in Bogor. The sample size was calculated using the formula to detect disease with the prevalence assumed to be 5% using 95% significant level. Milk and faeces samples were taken from 62 dairy cows which were suspected as suffering from MAP infection. Detection of MAP was done by isolation in Herrold’ egg yolk medium with mycobactin J (HEYMj), acid-fast bacilli Ziehl-Neelsen staining, PCR IS900 and F57. Biochemical test to confirm M. tuberculosis presence was also conducted. Fifteen isolates of Mycobacterium sp. were found from the faeces samples but not from the corresponding milk samples. However, conventional PCR conducted on the isolate as well as the milk samples, gave negative results. Biochemical test proved that all Mycobacterium sp. isolates were not M. tuberculosis. This study indicated the prevalence of MAP in Bogor was less than 5%. These findings should be continued by observational study to achieve the comprehensive information at the cattle and herd level. Bovine Tuberculosis monitoring should be done also to protect dairy herd and food safety for the community. Key words: Johne’s disease, Mycobacterium avium subspecies paratuberculosis, Milk, Faeces
Confirmation test of suspected Mycobacterium avium subspecies paratuberculosis (MAP) isolated using PCR F57 Nugroho, Widagdo Sri; Adji, Rahmat Setya; Wahyuni, Aeth
Indonesian Journal of Animal and Veterinary Sciences Vol 13, No 2 (2008)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.46 KB) | DOI: 10.14334/jitv.v13i2.605

Abstract

Seropositive and isolate suspected as Mycobacterium avium subspecies paratuberculosis (MAP) was detected at dairy cows in West Java. This bacteria causes Johne’s disease (JD) and potentially becomes a new emerging disease for Indonesian dairy cows. The aim of this study was to confirm the suspected local isolate as a MAP distinctively by PCR. Reculture of MAP reference isolate, suspected local isolate done by resuspending bacteria in PBS 0.5% and inoculating it in Herrold’s egg yolk medium with mycobactin J (HEYM) and than inoculating it in 37oC for 16 weeks. The cultures grew in various time, Mycobacterium avium subspecies avium was detected in 3rd week, MAP reference was detected in 7th week, and local isolate was detected in 14th week. The confirmation test was carried out by PCR with primer F57. The PCR F57 result showed that MAP suspected isolate was not a Mycobacterium avium subspecies paratuberculosis. Key Words: Local Isolate, Mycobacterium avium Subspecies Paratuberulosis, PCR F57
Confirmation test of suspected Mycobacterium avium subspecies paratuberculosis (MAP) isolated using PCR F57 Widagdo Sri Nugroho; Rahmat Setya Adji; Aeth Wahyuni
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 2 (2008): JUNE 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.46 KB) | DOI: 10.14334/jitv.v13i2.605

Abstract

Seropositive and isolate suspected as Mycobacterium avium subspecies paratuberculosis (MAP) was detected at dairy cows in West Java. This bacteria causes Johne’s disease (JD) and potentially becomes a new emerging disease for Indonesian dairy cows. The aim of this study was to confirm the suspected local isolate as a MAP distinctively by PCR. Reculture of MAP reference isolate, suspected local isolate done by resuspending bacteria in PBS 0.5% and inoculating it in Herrold’s egg yolk medium with mycobactin J (HEYM) and than inoculating it in 37oC for 16 weeks. The cultures grew in various time, Mycobacterium avium subspecies avium was detected in 3rd week, MAP reference was detected in 7th week, and local isolate was detected in 14th week. The confirmation test was carried out by PCR with primer F57. The PCR F57 result showed that MAP suspected isolate was not a Mycobacterium avium subspecies paratuberculosis. Key Words: Local Isolate, Mycobacterium avium Subspecies Paratuberulosis, PCR F57
Detection of Mycobacterium avium subspecies paratuberculosis of dairy cows in Bogor Widagdo Sri Nugroho; M. Sudarwanto; D.W Lukman; E. Setiyaningsih; E. Usleber
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 4 (2009): DECEMBER 2009
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (304.452 KB) | DOI: 10.14334/jitv.v14i4.335

Abstract

Johne’s disease (JD) or partuberculosis is a chronic granulomatous enteritis in ruminants caused by infection of Mycobacterium avium paratuberculosis subspecies (MAP). The disease has been detected serologically in Indonesia. It’s potential to spread to other herds and could create great economic losses. The objectives of current study were to detect MAP in milk and faeces of dairy cows as well as to evaluate the association between farm management factors and presence of the bacteria in dairy cows in Bogor. The sample size was calculated using the formula to detect disease with the prevalence assumed to be 5% using 95% significant level. Milk and faeces samples were taken from 62 dairy cows which were suspected as suffering from MAP infection. Detection of MAP was done by isolation in Herrold’ egg yolk medium with mycobactin J (HEYMj), acid-fast bacilli Ziehl-Neelsen staining, PCR IS900 and F57. Biochemical test to confirm M. tuberculosis presence was also conducted. Fifteen isolates of Mycobacterium sp. were found from the faeces samples but not from the corresponding milk samples. However, conventional PCR conducted on the isolate as well as the milk samples, gave negative results. Biochemical test proved that all Mycobacterium sp. isolates were not M. tuberculosis. This study indicated the prevalence of MAP in Bogor was less than 5%. These findings should be continued by observational study to achieve the comprehensive information at the cattle and herd level. Bovine Tuberculosis monitoring should be done also to protect dairy herd and food safety for the community. Key words: Johne’s disease, Mycobacterium avium subspecies paratuberculosis, Milk, Faeces
Co-Authors Abdulwahed Ahmed Hassan AETH Wahyuni Wahyuni, AETH Wahyuni Agnesia Endang Tri Hastuti Wahyuni Agustina Dwi Wijayanti Aris Haryanto Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bawono, Gigih D.W Lukman Defryana Rakebsa Denny Widaya Lukman Dian Bekti Hadi Masithoh Doddi Yudhabuntara Doddy Yudhabuntara Dyah Ayu Widiasih Dyah Ayu Widiasih E. Setiyaningsih E. Usleber Erwan Agus Purwanto Etih Sudarnika Ewald Usleber F. Lintang R. Daru Maya Fitriana, Ida Gallantiswara, Putu Cri Devischa Hari Kusnanto Heris Kustiningsih Heru Susetya Hilda Susiyanti Debora Berek Ismadi, Joko Kajang, Elphan Augusta Khrisdiana Putri Khrisdiana Putri, Khrisdiana Kusnanto, Hari M. Haryadi Wibowo M. Sudarwanto Maftuhah Nurbeti, Maftuhah Marla Anggita Mazdani Ulfah Daulay Minarwati Sudarwanto Mirnawati Sudarwanto Nurbeti, Maftuhah Prisha Lini Sivalingham Puspa Wikan Sari Raden Gagak Donny Satria Rahmat Setya Adji Rahmat Setya Adji Rahmi, Siwi Meylina Rochman Naim Roza Azizah Primatika Roza Azizah Primatika, Roza Azizah Salim, Devi Andreani Setiawan, Dwi Cahyo Budi Setyawan Budiharta Silalahi, Gustaf Eifel Surachmi Setyaningsih Teguh Budipitojo Toha, Larry Richard Wellem Widodo Suwito Widodo Suwito Widodo Suwito Widya Asmara Yatri Drastini Yuda Heru Fibrianto Yudhanto, Setyo