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All Journal Journal of Tropical Soils Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biology, Medicine, & Natural Product Chemistry BERITA BIOLOGI Makara Journal of Science
Novik Nurhidayat
Puslit Biologi LIPI, Cibinong Science Center
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Medicine & Pharmacology Public Health

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Journal : Biology, Medicine,

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Superoxide Anion Radicals Biosensor Based on Protein Extract from Deinococcus Radiodurans Immobilization by Glutaraldehyde Cross-Linked Afifi, Muhammad Ridho; Iswantini, Dyah; Nurhidayat, Novik; Saprudin, Deden
Biology, Medicine, & Natural Product Chemistry Vol 14, No 1 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.141.153-160

Abstract

A common enzyme for superoxide anion radicals biosensors is superoxide dismutase (SOD). Deinococcus radiodurans protein extract can replace the use of pure SOD enzymes for superoxide anion radicals biosensors. The purpose of this research is to determine the analytical performance of the superoxide anion radicals biosensor with protein extract of Deinococcus radiodurans immobilized on carboxymethylcellulose-gelatin-zeolit (CMC-G-Z) that is crosslinked by glutaraldehyde. The response surface method showed that the optimum condition for the biosensor was pH 7, protein extract concentration of 1075 µg/ml, zeolite concentration of 5 mg/ml, and glutaraldehyde concentration of 0.0042 M. Stability of the biosensor retained 67% of its sensitivity after use for 24 hours. The biosensor exhibits good analytical performance with a linear range from 0.1 – 0.8 mM, a detection limit of 77.84 µM, and a limit of quantification of 259.5 µM with a correlation coefficient of 0.9905.
Co-Authors Afifi, Muhammad Ridho Deden Saprudin Dewi, Ratih M Dita Ariyanti Dori Kusuma Jaya Dyah Iswantini Evi Triana Ghozali, Ali Aulia Hefni Effendi I MADE ARTIKA Kasim, Ernawati Kurniawan, Yehezkiel Steven MS, Yulia Atika Purwantiningsih Sugita Rahayu, Sri Hartin Sarjiya Antonius Yulinery, Titin