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All Journal HAYATI Journal of Biosciences Saintia Biologi Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati agriTECH JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Jurnal Penelitian Pendidikan IPA (JPPIPA) Abdimas Talenta : Jurnal Pengabdian Kepada Masyarakat Jurnal Pembelajaran dan Biologi Nukleus Jurnal Ilmiah Biologi UMA (JIBIOMA)
KIKI NURTJAHJA
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Sumatera Utara Jl. Bioteknologi No.1, Kampus USU, Medan, Indonesia 20155
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Physics Social Sciences

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Journal : Jurnal Penelitian Pendidikan IPA (JPPIPA)

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Preliminary Study on Potential Bacterial Species Introducing Antibacterial Agent from Sponge in Unggeh Island Central Tapanuli, Indonesia Abdillah, Latifah Ningrum; Suryanto, Dwi; Nurtjahja, Kiki; Mamangkey, Jendri
Jurnal Penelitian Pendidikan IPA Vol 10 No 7 (2024): July
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i7.8264

Abstract

Sponges have the potential to symbiont with bacteria that produce antibacterial compounds. This study was conducted to isolate and test the antibacterial activity of sponge symbiont bacteria. Bacterial isolates obtained were tested for antibacterial activity against Staphylococcus aureus, Bacillus subtilis and Escherichia coli using the Kirby-Bauer method. The identification results of both sponges have similarities with Clathria sp. and Hyrtios sp. symbiont bacteria obtained from both sponges as many as 16 isolates. Antibacterial activity testing had a weak inhibitory effect on isolates Sp1A, Sp2C, Sp2D and Sp2F, while Sp2J showed moderate inhibition against gram-negative and positive bacteria. The antibacterial activity of the whole supernatant of symbiont bacteria obtained the diameter of the inhibition zone with weak, medium and strong categories. Sp1A isolate supernatant was obtained, potentially able to fight Escherichia coli bacteria with a strong category as a representation of gram-negative bacteria and against gram-positive bacteria (Bacillus subtilis, Staphylococcus aureus) classified into the medium category. In conclusion, 16 symbiont bacterial supernatants have antibacterial activity mostly active against gram-negative bacteria Escherichia coli and gram-positive bacteria Bacillus subtilis. There are 6 isolates (Sp1A, Sp1B, Sp1E, Sp1F, Sp2D, Sp2F) active as antibacterial against Staphylococcus aureus with weak and moderate categories.
Antimicrobial Activity Test of Methanol Extract of Haliclona sp. from Sibolga Waters Mashitah, Ulfa; Suryanto, Dwi; Nurtjahja, Kiki
Jurnal Penelitian Pendidikan IPA Vol 10 No 11 (2024): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i11.8287

Abstract

Haliclona sp. is a genus of demosponges in the family Chalinidae. Demospongiae are a type of sponge that lives in marine environments and is known for its unique, often branching skeletal structure. The use of antibiotics has led to the selection of pathogenic microorganisms in humans that are more resistant to many types of drugs, resulting in poorer treatment outcomes in cases of infection. Bacteria can become resistant to antibiotics due to improper dosing, inaccurate diagnosis, and inappropriate use of antibiotics against the causative bacteria. This study aims to determine the antimicrobial activity of the methanol extract of Haliclona sp. as an inhibitor of pathogenic microbes. This research employs a descriptive method with antagonistic testing. The results of the methanol extraction process of the symbiotic bacteria in Haliclona sp. produced a thick, dark brown extract. The results of the antagonistic test showed that the clear zones formed on the three test microbes were categorized as strong.
Co-Authors Abdillah, Latifah Ningrum Agnes Dame Sinta Uli Berliani, Kaniwa Cut Fatimah Zuhra DAYAR ARBAIN DEDI PRIMA PUTRA DWI SURYANTO Elimasni Erman Munir Erni Jumilawaty Eryna Elfasari Rangkuti etti sartina siregar Ferdinand Susilo GINTA RIO Hesti Wahyuningsih Ida Fauziah Ida Fauziah, Ida Ira Wulan Dani Irene Banjarnahor Irene Banjarnahor, Irene Isnaini Nurwahyuni Jendri Mamangkey Julius Paolo Siregar Kardhinata, Harso Lisa Novita Arios Lubis, Nurul Abdillah Mashitah, Ulfa Masitta Tanjung Muhammad Yusuf Muhammad Yusuf Nasution, MHD. Fauzi Novi Malinda NUNUK PRIYANI Okky Setyawati Dharmaputra Putri, Mimmy Sari Syah Rahmiati Rahmiati Rahmiati Rahmiati Riska Annisa Putri Riyanto Riyanto Rizal Syarief Rosliana Lubis Rosliana Lubis, Rosliana Sartini Sartini Sri Dwi Hastuti Srimaulinda Srimaulinda Supriharti, Deny TATA BINTARA KELANA Winda Winda Winiati Pudji Rahayu Yurnaliza, Yurnaliza Zebua, Winda Irawati