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All Journal Jurnal Sain Veteriner Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Jurnal Bisnis dan Manajemen Wahana Peternakan Journal of Livestock and Animal Health (JLAH)

Fajar Budi Lestari

Departement Of Bioresources Technology And Veterinary, Vocational College, Universitas Gadjah Mada, Yogyakarta, Indonesia

Author-ID : 3368610

Agriculture, Biological Sciences & Forestry Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Veterinary

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Klasterisasi Staphylococcus aureus Resisten Neutrofil Berdasar Assesory Gene Regulator Santosa, Christin Marganingsih; Lestari, Fajar Budi; Widayanti, Rini; Salasia, Siti Isrina Oktavia
Jurnal Sain Veteriner Vol 38, No 2 (2020): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.50653

Staphylococcus aureus is recognized worldwide as a major pathogen causing subclinical intramammary infections in dairy cows and food poisoning due to its ability to produce enterotoxin. The study aimed to identify enterotoxins of S. aureus and clustering the enterotoxins based on assessory gene regulator (agr). Virulence of S. aureus to the host was characterized based on the response of polymorphonuclear cells to the infection. Twelve S. aureus could be isolated from milk cows in central of dairy farming in Sumedang West Java. The identification of S. aureus was based on cultural and biochemical tests and an amplification of a specific section of the 23S rRNA gene. The sensitivity test against antibiotics revealed that some isolates of S. aureus were resistant to penicillin and methycillin. By PCR amplification one or more staphylococcal enterotoxin genes could be observed five genes in combinations of sea (216 bp), seb (478 bp), seh (375 bp), sei (576 bp), and sej (142 bp). Clustering of S. aureus based on the assesory gene regulator could be grouped into 4 clusters for agr1 (1 isolat), agr2 (2 isolates), in combination for agr1 and agr2 (1 isolate), and for non agr (2 isolates). Based on the response of polymorphonuclear cell in vitro and in vivo assays, revealed that S. aureus strain I-2 (agr1 cluster) and P1 (agr1+agr2 cluster) were more resistant to polymorphonuclear cells and could survive intracellularly, indicated that these strains could be used as proper candidates to develop dignostic tool based on agr against staphylococcal mastitis.

Karakterisasi Staphylococcus aureus Isolat Susu Sapi Perah Berdasar Keberadaan Protein-A pada Media Serum Soft Agar terhadap Aktivitas Fagositosis Secara In Vitro Fajar Budi Lestari; Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 33, No 2 (2015): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Staphylococcus aureus merupakan salah satu bakteri penyebab utama mastitis. Protein-A berperan penting dalam adesi dan kolonisasi bakteri pada sel inang. Penelitian ini bertujuan untuk mengetahui hubungan aktivitas fagositosis S. aureus berdasarkan keberadaan protein-A pada media serum soft agar. Sebanyak 19 isolat S. aureus susu sapi perah asal Jawa Barat dan Jawa Tengah digunakan pada penelitian ini. Seluruh isolat tersebut direidentifikasi dengan dipupuk pada media plat agar darah (PAD), koloni bakteri kemudian diidentifikasi dengan pewarnaan Gram, uji mannitol salt agar (MSA), katalase dan uji koagulase. Karakterisasi S.aureusdilakukan dengan menanam bakteri pada media serum soft agar (SSA) yang mengandung serum kelinci untuk mengetahui keberadaan protein-A, kemudian dilakukan uji fagositosis dengan menggunakan sel polimorfonuklear. Dari 19 isolat tersebut seluruhnya teridentifikasi sebagai S. aureus yang ditunjukkan dengan Gram positif, sel berbentuk kokus bergerombol, mampu memfermentasi manitol pada media MSA, positif pada uji katalase, 15,79% sampel menunjukkan hasil koagulase negatif, sedangkan 84,21% menunjukkan hasil koagulase positif. Pertumbuhan pada media SSA menunjukkan hasil 12 isolat (63,16%) koloni berbentukkompak dan 7 isolat (36,84%) koloni berbentuk difus. Koloni kompak menunjukkan bakteri tersebut memiliki protein-A, koloni difus menunjukkan bakteri tersebut tidak memiliki protein-A atau memiliki protein-A tetapi tertutup oleh kapsul. Hasil uji fagositosis menunjukkan S. aureus yang memiliki protein-A lebih sedikit difagosit oleh leukosit polimorfonuklear (2,99 bakteri/sel) dari pada S. aureus yang tidak memiliki protein-A, atau mempunyai protein-A tetapi tertutup oleh kapsul (3,85 bakteri/sel). Staphylococcus aureus yang memiliki protein-A lebih patogen daripada S. aureus yang tidak memiliki protein-A. Isolat S. aureus asal Jawa Tengah lebih virulen dibandingkan isolat S. aureus asal Jawa Barat ditinjau dari sifat hemolisis, koagulase, dan protein-A.

Deteksi Gen Penyandi Sifat Resistensi Metisilin, Penisilin dan Tetrasiklin pada Isolat Staphylococcus aureus Asal Susu Mastitis Subklinis Sapi Perah Fatkhanuddin Aziz; Fajar Budi Lestari; Sarah Nuraidah; Endah Purwati; Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Detection of gene encoding resistance of bacteria could be used as an accurate method to determine resistance of Staphylococcus aureus which is causing mastitis in dairy cows to the several antibiotics. This research aimed to detect the gene encoding resistance of methicillin, penicillin and tetracycline from identified S. aureus. Sixty milk samples were collected from subclinical mastitis of cows from various dairy farming in Yogyakarta. Isolation and identification of S. aureus based on the culture, Gram staining and biochemical test. Phenotypes of S. aureus resistances against antibiotics were carried out by disc diffusion method, meanwhilespecies specific gene of S. aureus and the gene encoding methicillin, penicillin and tetracycline were confirmed by PCR method. The results showed 11 isolates representing of Methicillin Susceptible Staphylococcus aureus (MSSA) could be identified, wherein 5 isolates were harboring both of penicillin and tetracycline resistant genes respectively.

Potensi Ekstrak Daun Sage (Salvia officinalis.L) sebagai anti-Streptococcus suis Penyebab Zoonotik Meningitis Mitra Slipranata; Fajar Budi Lestari; Novra Arya Sandi; Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 34, No 2 (2016): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The phenomenon of microbial resistance to an antibiotic or some kind of specific antibiotics (multi drugs resistance) greatly complicate the treatment process, one of which is Streptococcus suis (S. suis) which is known to cause meningitis in animals and humans. Today, with rising bacterial resistance to a wide rangeof antibiotics, it takes an effort to assess the potential medicinal plants as an antibiotic that is appropriate and safe. Sage (Salvia officinalis.L) is reported to have antibacterial and fungicidal effect. Phenolic acids such assalvin and ether monomethyl salvin which isolated from the sage thought to have antimicrobial activity against several bacterial strains. The purpose of this study was to prove the ability of sage leaf extract as an antibacterialagainst S. suis causes streptococcal meningitis in vitro. In-vitro method used in this research through a two-stage dilution test and the diffusion test on Mueller Hinton Agar (MHA). S. suis isolates (code 225) were tested in vitro against 8 levels sage leaf extract concentration, ie a concentration of 1%, 3% 5%, 7%, 10%, 20%, 40% and 60%. The results of the test obtained by minimum inhibitory concentrations (MICs) and minimum sage known to potentially inhibit the growth of S. suis.

Klebsiella pneumoniae Isolated from Subclinical Mastitis Milk of Etawah Crossbreed Goat Clara Ajeng Artdita; Fajar Budi Lestari; Achmad Fauzi; Erian Pemila Ayu Tanzila
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Klebsiella spp. is a common bacteria causing mastitis. Klebsiella pneumoniae and Klebsiella varicola is the most infected Klebsiella spp in the dairy farm. This study used 16 milk samples from 8 lactation goats in Sahabat Ternak farm, Sleman, Yogyakarta. Samples were tested by california mastitis test (CMT) reagent to determine the mastitis status, inoculated on blood agar (BA) then on Mac Conkey Agar (MCA) as a selective media for Gram negative bacteria. The colonies from BA and MCA were stained by Gram staining to determine Gram-positive or Gram-negative bacteria and its cell morphology. The colonies from MCA were identified by biochemical tests such as sugar fermentation tests (glucose, lactose, maltose, and saccharose), arginine, ornithine decarboxylase, indole, citrate, urea hydrolysis (Christensen’s method), lysin and malonate. The result of mastitis test showed 5 goats were positive result and 3 samples were negative. Bacterial staining showed 2 samples were Gram-negative, rods and the others were Gram-positive, coccus. The samples with rods shapes were continued by biochemical tests. The characterization result of biochemical test indicated that the rods shapes bacteria were Klebsiella pneumoniae. These bacteria identification conducted in Laboratorium Preklinis Program Studi Kesehatan Hewan, Sekolah Vokasi Universitas Gadjah Mada and the result confirmation using positive control of Klebsiella pneumoniae conducted in Balai Laboratorium Kesehatan Yogyakarta. These results showed that milk from Etawah crossbreed goat was infected by Klebsiella pneumoniae.

Potensi Ekstrak Atuna racemosa sebagai Anti - Methicillin Resistant Staphylococcus aureus (MRSA) Siti Isrina Oktavia Salasia; Novra Arya Sandi; Fajar Budi Lestari; Verda Farida; Nurbani Aziz
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Staphylococcus aureus is one of the major causative agents of mastitis in animals and a variety of human diseases such as septicemia, endocarditis, arthritis dan osteomyelitis. Infection of Methicillin-resistant S. aureus (MRSA) has been widely reported and these strains are usually resistant to multiple antibiotics. The purpose of this study was to evaluate the potential of Atuna racemosa, as an alternative herbal medicine against MRSA infection. The MRSA strains were isolated from human and confirmed based on their resistant to various antibiotics and analyzing of the mecA gene by polymerase chain reaction (PCR). Atuna racemosa originated from Ambon, Maluku, Indonesia, were extracted using 70% ethanol. The activities of the Atuna racemosa extract against MRSA were performed by diffusion disc agar and dilussion agar tests. The results showed that Atuna racemosa extract has the barrier effect of MRSA growth at a concentration of 5% in the diffusion test and at a concentration of 7% in the dilution test. Atuna racemosa could be used as an alternative new drugs with dose of 0.07 g/ml (7%) against MRSA which is multi-resistant to many antibiotics.

Deteksi Staphylococcus aureus dan Staphylococcus sp. Secara Langsung Dari Susu Segar Kambing Peranakan Etawa dengan Teknik PCR Fatkhanuddin Aziz; Fajar Budi Lestari; Sarah Nuraida S; Endah Purwati; Siti Isrina Oktavia Salasia
Jurnal Sain Veteriner Vol 38, No 2 (2020): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.53802

Genus Staphylococcus merupakan salah satu patogen bakteri penyebab mastitis yang menyebabkan kerugian ekonomi pada kambing Peranakan Etawa. Diantara Staphylococcus sp. yang dapat tumbuh dengan baik dalam susu segar, diketahui Staphylococcus aureus dapat membahayakan kesehatan manusia yang mengkonsumsi (food borne disease) karena kemampuannya dalam memproduksi enterotoksin yang tahan terhadap enzim pencernaan maupun pemanasan. Tujuan penelitian ini adalah mendeteksi Staphylococcus sp. dan S. aureus secara langsung dari susu kambing peranakan etawa dengan teknik PCR.Metode yang dilakukan adalah mengekstraksi DNA dari 60 sample susu segar dengan prinsip spin column-based nucleic acid purification dan kemudian dilakukan amplifikasi gen spesifik 23S rRNA Staphylococcus sp. dan S. aureus. Hasil PCR diketahui 37 (61%) sampel susu positif mengandung Staphylococcus sp. dan hanya 1 (1,6%) sampel mengandung S. aureus. Metode deteksi dengan PCR dapat digunakan untuk mendeteksi kontaminan Staphylococcus sp. dan S. aureus dengan waktu yang singkat

Variasi Karakter Resistensi Antibiotik Bakteri Penyebab Mastitis Subklinis pada Kambing Peranakan Ettawa di Yogyakarta Lestari, Fajar Budi; Artdita, Clara Ajeng; Wasissa, Madarina; Hidayah, Nurulia
Jurnal Sain Veteriner Vol 43, No 1 (2025): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.52529

Salah satu penyakit yang sering dijumpai dalam budidaya kambing Peranakan Etawa (PE) adalah mastitis. Beberapa bakteri patogen dapat menyebabkan mastitis diantaranya adalah Staphylococcus sp., E. coli, Klebsiella spp., dan Streptococcus spp. Meningkatnya kejadian penyakit menyebabkan meningkatnya penggunaan antibiotik yang berakibat pada peningkatan potensi resistensi dan residu antibiotik. Penelitian ini bertujuan untuk mengetahui variasi karakter fenotipik resistensi bakteri penyebab mastitis yang diisolasi dari susu kambing PE yang mengalami mastitis subklinis terhadap berbagai golongan antibiotik yang umum dipakai petugas medis veteriner di wilayah Yogyakarta. Sebanyak 8 isolat diperoleh dari peternakan rakyat di wilayah kecamatan Kokap, Kulon Progo,, sedangkan 4 isolat diperoleh dari wilayah Pakem, Sleman, Yogyakarta. Dua belas isolat tersebut terdiri dari 3 jenis bakteri yang berbeda yaitu Staphylococcus sp., Escherichia coli, dan Klebsiella sp. Berdasarkan hasil uji antibiogram diketahui bahwa terdapat perbedaan pola resistensi antibiotik antara isolat bakteri penyebab mastitis dari Kulon Progo dan Sleman. Ampicillin, penicillin G, tetracyclin, dan cefoxitin efektif digunakan untuk pengobatan mastitis di daerah Sleman, sedangkan di Kulon Progo gentamicyn, tetracyclin dan erythromycin adalah pilihan antibiotik yang efektif digunakan

Literatur Review: Analisis Risiko, Peneguhan Diagnosa dan Evaluasi pada Feline Panleukopenia Virus Maharani, Ananda Peurine Syafira; Yuliawati, Priskilla Sandi; Yenni, Latifah; Lestari, Fajar Budi
Jurnal Sain Veteriner Vol 43, No 1 (2025): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.92291

Feline Panleukopenia Virus (FPV) merupakan penyakit infeksius yang dapat menginfeksi hewanFamili Felidae khususnya kucing. Virus ini mempunyai tingkat mortalitas dan morbiditas yang sangat tinggi terlebih pada kucing yang belum divaksinasi. Literatur review ini membahas tentang analisis risiko berbagai metode yang umum digunakan untuk membantu peneguhan diagnosis dan evaluasi yang dapat dilakukan pada penderita FPV. Metodeyang digunakan adalah studi literatur yaitu dengan mengumpulkan dan merangkum jurnal-jurnal dengan topik relevan yang ditelusuri dari Google Scholar, NCBI, Library Genesis, PubMed, Science Direct dan Europe PMC. Berdasarkan penelitian, didapatkan hasil diantaranya bahwa FPV akan menunjukkan gejala klinis yang beragam mulai dari subklinis, akut, hingga perakut. Vaksinasi merupakan faktor utama yang mempengaruhi infeksi FPV. Metode yang umum dilakukan untuk membantu peneguhan diagnosis antara lain imunokromatografi; hematologi dan biokimia; Polymerase Chain Reaction (PCR); ELISA; dan histopatologi. Evaluasi yang dapat dilakukan untuk penderita FPV yaitu berupa upaya preventif dan suportif.

Isolasi dan Identifikasi Methicillin Resistant Staphylococcus aureus dan Methicillin Resistant Staphylococcus pseudintermedius pada Kasus Klinis Anjing dan Kucing di Yogyakarta Alhadz, Ghias Ghifari; Salasia, Siti Isrina Oktavia; Yosyana, Alyaa Rifqoh Putri; Wasissa, Madarina; Lestari, Fajar Budi; Widayanti, Rini
Jurnal Sain Veteriner Vol 43, No 2 (2025): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.100240

Methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP), are known as bacterial resistant to various antibiotics. MRSA transmission occurs between humans and direct human contact with dogs and cats. This study aimed to isolate, characterize, and analyze bacterial resistance to antibiotics phenotypically and genotypically from clinical case samples of pet animals in Yogyakarta. Bacterial isolates were identified through biochemical tests and molecular identification by species-specific 23S rRNA and nuc genes with polymerase chain reaction (PCR) and pta genes with PCR-restriction fragment length polymorphism (RFLP). Bacterial resistance to several antibiotics was analyzed using disk diffusion Kierby-Bauer method. The occurrence of methicillin resistance were screened by oxacillin resistance screening agar base (ORSAB) and confirmed by detection of the mecA gene with PCR. Based on the phenotypic and genotypic identification showed that 11 samples were identified as S. aureus (6 isolates) and S. pseudintermedius (5 isolates). The resistance tests revealed that 36% were resistant to tetracycline, cefoxitin (27%), oxacillin and erythromycin (9%). Screening of methicillin resistance with ORSAB indicated that all isolates were methicillin resistances (100%). Detecting the mecA gene encoding methicillin resistance showed that 81.81% of isolates were resistant to methicillin, with detail S. aureus (all 6 isolates) and S. pseudintermedius (4 isolates). Based on the antibiogram, ORSAB, and mecA gene detection, it could be confirmed that three isolates were MRSP, and six isolates were MRSA. The results of this study indicate the high occurrence of methicillin-resistant strains in pets that have the potential zoonotic spread to other animals and humans.

Co-Authors Achmad Fauzi Achmad Fauzi Alhadz, Ghias Ghifari Anggraini, Mila Putri Budiyanto, Yohanes Wawan Christin Marganingsih Santosa Clara Ajeng Artdita Clara Ajeng Artdita Clara Ajeng Artdita, Clara Ajeng Dewi, Vira Kartika Donny Widianto Endah Purwati Endah Purwati Erian Pemila Ayu Tanzila Fatkhanuddin Aziz, Fatkhanuddin Fitriana, Fauziah Hidayah, Nurulia Hidayatullah, Muhammad Fatan Kustika, Estha Gusmalia Madarina Wasissa Maharani, Ananda Peurine Syafira Maulana, Husni Maulina, Tifa Restyka Mitra Slipranata Morsid Andityas Muhammad Novrizal Abdi Sahid Novra Arya Sandi Novra Arya Sandi Nurbani Aziz NURULIA HIDAYAH Prihanani, Nur Ika Putri, Shafira Amalia Rahmayanti, Desyah Rarastoeti Pratiwi Rini Widayanti Risa Ummami, Risa Sarah Nuraida S Sarah Nuraidah Setyorini, Dian Ritma Siti Isrina Oktavia Salasia Siti Isrina Oktavia Salasia Siti Isrina Oktavia Salasia Siti Isrina Oktavia Salasia Wasissa, Madarina Yenni, Latifah Yosyana, Alyaa Rifqoh Putri Yuliawati, Priskilla Sandi

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