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All Journal International Journal of Electrical and Computer Engineering TELKOMNIKA (Telecommunication Computing Electronics and Control) Jurnal Ilmiah Teknik Elektro Komputer dan Informatika (JITEKI) Dental Journal (Majalah Kedokteran Gigi) Journal of Indonesian Dental Association MDJ (Makassar Dental Journal) Jurnal Radiologi Dentomaksilofasial Indonesia (JRDI) Indonesian Journal of Dental Medicine Budapest International Research in Exact Sciences (BirEx Journal) Jurnal Pengabdian Nasional (JPN) Indonesia Indian Journal of Forensic Medicine & Toxicology Jurnal Pengabdian Masyarakat Indonesia Jurnal Pengabdian Nasional (JPN) Indonesia Jurnal of Syiah Kuala Dentistry Society
Eha Renwi Astuti
Department Of Dentomaxillofacial Radiology, Faculty Of Dental Medicine Universitas Airlangga
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Physics Public Health Social Sciences Transportation Other

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Journal : Dental Journal (Majalah Kedokteran Gigi)

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The role of heat shock protein (HSP ) as inhibitor apoptosis in hypoxic conditions of bone marrow stem cell culture Sri Wigati Mardi Mulyani; Ernie Maduratna Setiawati; Erma Safitri; Eha Renwi Astuti
Dental Journal (Majalah Kedokteran Gigi) Vol. 47 No. 1 (2014): March 2014
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (535.11 KB) | DOI: 10.20473/j.djmkg.v47.i1.p41-44

Abstract

Background: The concept of stem cell therapy is one of the new hope as a medical therapy on salivary gland defect. However, the lack of viability of the transplanted stem cells survival rate led to the decrease of effectiveness of stem cell therapy. The underlying assumption in the decrease of viability and function of stem cells is an increase of apoptosis incidence. It suggests that the microenvironment in the area of damaged tissues is not conducive to support stem cell viability. One of the microenvironment is the hypoxia condition. Several scientific journals revealed that the administration of hypoxic cell culture can result in stress cells but on the other hand the stress condition of the cells also stimulates heat shock protein 27 (HSP 27) as antiapoptosis through inhibition of caspase 9. Purpose: The purpose of this study was to examine the role of heat shock protein 27 as inhibitor apoptosis in hypoxic conditions of bone marrow stem cell culture. Methods: Stem cell culture was performed in hypoxic conditions (O2 1%) and measured the resistance to apoptosis through HSP 27 and caspase 9 expression of bone marrow mesenchymal stem cells by using immunoflorecence and real time PCR. Results: The result of study showed that preconditioning hypoxia could inhibit apoptosis through increasing HSP 27 and decreasing level of caspase 9. Conclusion: The study suggested that hypoxic precondition could reduce apoptosis by increasing amount of heat shock protein 27 and decreasing caspase 9.Latar belakang: Konsep terapi stem cell merupakan salah satu harapan baru sebagai terapi medis kelainan kelenjar ludah. Namun, rendahnya viabilitas stem cell yang ditransplantasikan menyebabkan penurunan efektivitas terapi. Asumsi yang mendasari rendahnya viabilitas dan fungsi stem cell adalah tingginya kejadian apoptosis. Hal ini menunjukkan bahwa lingkungan mikro di daerah jaringan yang rusak tidak kondusif untuk mendukung viabilitas stem cell. Salah satu lingkungan mikro adalah kondisi hipoksia. Beberapa jurnal ilmiah mengungkapkan bahwa kondisi hipoksia pada kultur sel dapat menyebabkan sel-sel stres, namun di sisi lain kondisi stres sel juga merangsang heat shock protein 27 (HSP 27) sebagai antiapoptosis dengan menghambat ekspresi caspase 9. Tujuan: Tujuan penelitian ini adalah untuk meneliti peran protein heat shock 27 sebagai inhibitor apoptosis dalam kondisi hipoksia kultur stem cell sumsum tulang. Metode: Kultur stem sel dilakukan dalam kondisi hipoksia (O2 1%) dan mengukur resistensi terhadap apoptosis melalui ekspresi HSP 27 dan caspase 9 stem cell mesenchymal sumsum tulang dengan menggunakan immunoflorecence dan PCR real time. Hasil: Hasil penelitian menunjukkan bahwa prakondisi hipoksia dapat menghambat apoptosis melalui peningkatan HSP 27 dan penurunan tingkat Caspase 9. Simpulan: Studi ini menunjukkan bahwa prakondisi hipoksia dapat mengurangi apoptosis dengan meningkatkan jumlah protein heat shock 27 dan penurunan caspase 9.
VEGF expression and new blood vessel after dental X-ray irradiation on fractured tooth extraction wound Niluh Ringga Woroprobosari; Jenny Sunariani; Eha Renwi Astuti
Dental Journal (Majalah Kedokteran Gigi) Vol. 48 No. 3 (2015): September 2015
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1668.229 KB) | DOI: 10.20473/j.djmkg.v48.i3.p159-164

Abstract

Background:Dental X-ray has an important role in dentistry. Complication case such as tooth fracture extraction requires this examination to determine the appropriate treatment measures. Dental X-ray can also cause a negative impact to the body at cellular and even molecular level. Purpose: The aim of this study was to evaluate the decrease of vascular endothelial growth factor (VEGF) expression and new blood vessels number caused by dental X-ray irradiation on fractured tooth extraction wound on day 3 and 7 after extraction. Method: We used 30 wistar rats which was randomly divided into 6 groups. Each rat’s central insisive of left mandible was fractured and then extracted after or without X-ray irradiation. Group KA and KB were control groups without irradiation. Group P1 A and P1 B were treatment groups with 0.08 mSv irradiation dose. Group P2 A and P2 B were treatment groups with 0.16 mSv irradiation dose. The subject from group KA, P1 A, and P2 A were sacrficed and sockets were collected at day 3. The subject from group KB, P1 B, and P2 B were sacrficed and sockets were collected at day 7. Socket were processed and painted with hematoxylin eosin and immunohistochemistry, then observed with a microscope. Data processing was performed with SPSS 16 through one way anova test and post hoc Tukey test HS. Result: The lowest means expression of VEGF and the number of new blood vessels on the day 3 was found in P2 A group, and the highest found in the KA group. The lowest means expression of VEGF and the number of new blood vessels on the day 7 was found in P2 B group, and the highest found in the KB group. Conclusion: Dental X-ray irradiation dose of 0.08 mSv and 0.16 mSv causes decrease of VEGF expression and new blood vessels in the wound fractured tooth extraction in day 3 and day 7 post-extraction.
Transforming growth factor beta 1 expression and inflammatory cells in tooth extraction socket after X-ray irradiation Ramadhan Hardani Putra; Eha Renwi Astuti; Rini Devijanti Ridwan
Dental Journal (Majalah Kedokteran Gigi) Vol. 49 No. 2 (2016): June 2016
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (870.269 KB) | DOI: 10.20473/j.djmkg.v49.i2.p87-92

Abstract

Background: Radiographic examination is often used in dentistry to evaluate tooth extraction complications. X-ray used in radiographic examination, however, has negative effects, including damage to DNA and inflammatory response during wound healing process. Purpose: This study aimed to analyze the effects of X-ray irradiation on transforming growth factor beta 1 (TGF-ß1) expression and number of inflammatory cells in tooth extraction sockets. Method: Thirty rats were divided into three groups, which consist of control group (with a radiation of 0 mSv), treatment group 1 (with a radiation of 0.08 mSv), and treatment group 2 (with a radiation of 0.16 mSv). These rats in each group were sacrificed on days 3 and 5 after treatment. Inflammatory cells which were observed in this research were PMN, macrophages, and lymphocytes. Histopathological and immunohistochemical examinations were used to calculate the number of inflammatory cells and TGF-ß1 expression. Obtained data were analyzed using SPSS 16.0 software with one way ANOVA and Tukey’s HSD tests. Result: There was no significant decrease in the number of PMN. On the other hand, there were significant decreases in the number of macrophages and lymphocytes in the sacrificed group on day-5 with the radiation of 0.16 mSv. Similarly, the most significant decreased expression of TGF-ß1 was found in the group sacrificed on day 5 with the radiation of 0.16 mSv. Conclusion: X-ray irradiation with 0.08 mSv and 0.16 mSv doses can decrease TGF-ß1 expression and number of inflammatory cells in tooth extraction sockets on day 3 and 5 post extraction.
Changes in the number of macrophage and lymphocyte cells in chronic periodontitis due to dental X-ray exposure Alhidayati Asymal; Eha Renwi Astuti; Rini Devijanti
Dental Journal (Majalah Kedokteran Gigi) Vol. 51 No. 2 (2018): June 2018
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v51.i2.p99-103

Abstract

Background: Periodontitis is an inflammatory disease caused by specific microorganisms that attacks tooth-supporting tissues, P. gingivalis bacteria are mostly found in patients suffering from chronic periodontitis which is usually diagnosed by means of clinical and radiographic examination. The latter play important roles in the management of periodontitis, including: establishing diagnosis, determining treatment plans and evaluating the results of treatment. Unfortunately, the use of X-rays to perform such radiographic examination has negative effects since the body’s various parts, especially the head, are not well protected from the effects of X-ray radiation. Purpose: This research aimed to analyze the effects of dental X-ray exposure on the number of macrophages and lymphocytes in experimental subjects suffering from periodontitis. Methods: 36 rats that had been diagnosed with chronic periodontitis were divided into three groups, namely: a control group, treatment group I (exposed to a 0.16 mSv dose of radiation) and treatment group II (exposed to a 0.32 mSv dose of radiation). These subjects were subsequently sacrificed on the third and fifth days after treatment. Thereafter, histopathological examination was performed to identify any changes in the number of macrophages and lymphocytes. Results: The results of an HSD test confirmed that, on the third day, there were significant differences in the number of lymphocytes between the control group and treatment group I, as well as between the control group and treatment group II. On the fifth day, there were also significant differences in the number of lymphocytes between the control group and treatment group I, as well as between treatment group I and treatment group II. Similarly, there was a significant difference in the number of macrophage cells on the third day between the control group and treatment group I. On the fifth day, there were also significant differences in the number of macrophage cells between the control group and treatment group I, as well as between treatment group I and treatment group II. Conclusion: Dental x-ray exposure at a dose of 0.16 mSv can elevate the number of macrophages and lymphocytes on the third and fifth days. On the other hand, dental x-ray radiation at a dose of 0.32 mSv can reduce the number of macrophages on day 3 as well as the number of lymphocytes on the third and fifth days.
The effect of X-ray irradiation to the formation of polychromatic erythrocyte cell micronucleus in Wistar rats (Rattus norvegicus) Eha Renwi Astuti; Hutojo Djajakusuma; Indeswati Diyatri; Nastiti Faradilla Ramadhani
Dental Journal (Majalah Kedokteran Gigi) Vol. 54 No. 3 (2021): September 2021
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v54.i3.p128-131

Abstract

Background: Panoramic and cephalometric radiography is very important for diagnosis, treatment plan, and evaluation of orthodontic treatment results. Panoramic and cephalometric radiography are frequently performed at the same time, causing DNA damage and chromosome aberration. Purpose: This study aims to analyse the effect of X-ray exposure in panoramic and cephalometric radiography on micronuclei cell numbers. Methods: Laboratory-based analytical study with 60 healthy-male Wistar rats weighing 200–300 grams divided into 6 treatment groups (n=10). The control group: without radiographic exposure, the treatment group 2: using panoramic radiographic exposure followed by cephalometric, and the treatment group 3: using panoramic radiographic exposure and 24 hours later performed cephalometric radiographic. The unit of analysis was the polychromatic erythrocytes of mice cell, were examined 24 hours and 48 hours after irradiation had been finished. The polychromatic erythrocytes were examined using May-Gruenwald-Giemsa staining and 100x magnification under a microscope with 2000 cells per view. Data obtained were analysed using the SPSS 20 version software. The mean and standard deviations were calculated for each clinical parameter, and a one‐way ANOVA statistical test of significance was used. Statistical significance was set at p<0.05. Results: The analysis showed a significant increase (p<0.05) in the number of micronucleus in groups that used panoramic radiographic exposure followed by cephalometric. Conclusion: X-ray radiation can increase the number of micronucleus in polychromatic erythrocyte cells in rats.
Improperly diagnosed odontogenic myxoma in a 23-year-old female: A radiographic analysis Sugianto, Gabriel Maria Ferdilia; Nurrachman, Aga Satria; Astuti, Eha Renwi
Dental Journal (Majalah Kedokteran Gigi) Vol. 58 No. 1 (2025): March
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v58.i1.p88-94

Abstract

Background: Misdiagnosis can occur due to various radiographic alterations linked to odontogenic myxoma (OM). Regular examination can detect abnormalities early on, but not all practitioners are aware that these lesions exist. Purpose: This case report aims to describe and discuss an OM case from the perspective of oral radiology on panoramic radiographs and cone-beam computed tomography (CBCT). Case: A 23-year-old female went to her first dentist for orthodontic treatment with no prior radiographic evaluation. On January 7th, 2022, the second dentist extracted teeth 38 and 48 using the panoramic radiograph without identifying lesions. Concerned about swelling on her lower right gingiva, which had gradually grown, the patient went to an oral and maxillofacial surgeon on November 15th, 2022. The clinical examination revealed facial asymmetry with a thick, palpable, firm mass with an ambiguous boundary. Despite the evident movement of tooth 47, the gingiva exhibited no noticeable change in coloration. Case management: From the panoramic examination, multilocular radiolucency with radiopaque septa and aggressive mass characteristics were found. Advanced imaging CBCT was used to investigate further and correlate histology findings for treatment. Conclusion: Odontogenic myxoma is difficult to distinguish from other benign and malignant neoplasms due to the wide variations of radiological patterns. Cone-beam computed tomography provides a thorough and broad range of data that can be used to make a precise diagnosis and develop an effective treatment strategy. This highlights the critical need for a trained expert to thoroughly examine CBCT scans.
Co-Authors Adiningsih, Adiningsih Aga Satria Nurrachman Agus Subhan Akbar, Agus Subhan Agus Zainal Arifin Alhidayati Asymal Arif Mudi Priyatno Ayu Ismi Hanifah Azhari Azhari Azifah Shafa Izdihar Baskoro Nugroho Berty Pramatika Bosawer, Ellyonord Diana Bremmy Laksono Chastine Fatichah Darlis Herumurti Deny Saputra Diana Lilik Zainiyyah Dina Karimah Putri Dini Adni Navastara, Dini Adni edy susanto Erma Safitri Ernie Maduratna Setiawati Esa Prakasa Evan Tanuwijaya Fadhlil Ulum Abdul Rahman Faikoh, Nur Laili Izzatul Farah Tariza Harlens Hapzi Ali Haris Nasutianto Haryono Utomo Hilda Majidah Hutojo Djajakusuma Indeswati Diyatri Jenny Sunariani Kemala Hayati Khusnul Rahmawati Lailatul Hikmah Mazlan Lashari, Dur Muhammad Lusi Epsilawati Mieke Sylvia Nafiiyah, Nur Nastiti Faradilla Ramadhani Niluh Ringga Woroprobosari Nur Nafi’iyah Putra, Ramadhan Hardani Putri, Kharisma Gaprila Rarasmaya Indraswari Ratu Sofia Nur Aini Ricardo Henrique Alves da Silva Riki Kristanto Rini Devijanti Rini Devijanti Ridwan Sarianoferni Sarifah, Norlaila Sri Wigati Mardi Mulyani Sugianto, Gabriel Maria Ferdilia Tri Martini Umi Lutfiah Wahjuni, Otty Ratna Wahyuni, Otty Ratna Yosi Kristian Yunita Savitri