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All Journal Jurnal Rekayasa Proses Reaktor Research Report - Engineering Science Jurnal Pendidikan Indonesia Jurnal Bahan Alam Terbarukan Seminar Nasional Teknik Kimia Kejuangan Jurnal Rekayasa Proses Jurnal Teknik Kimia Indonesia Journal of Multidisciplinary Applied Natural Science Asia Pacific Journal of Management and Education INDONESIAN JOURNAL OF URBAN AND ENVIRONMENTAL TECHNOLOGY Jurnal Rekayasa Proses
Susiana Prasetyo S
Jurusan Teknik Kimia, Fakultas Teknologi Industri Universitas Katolik Parahyangan, Bandung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Control & Systems Engineering Education Energy Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Physics Social Sciences Other

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Journal : Seminar Nasional Teknik Kimia Kejuangan

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The Optimization of Bioactive Compounds Continuous Extraction Conditions from Phaleria macrocarpa Fruit by Percolation Method Susiana Prasetyo
Prosiding Seminar Nasional Teknik Kimia "Kejuangan" 2015: Prosiding SNTKK 2015
Publisher : Seminar Nasional Teknik Kimia Kejuangan

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Abstract

Crown of god (Phaleria macrocarpa) one of many native Indonesia medicinal plants, posseses with a great potential as natural preservative, food suplement, and medicine due to its high bioactive compounds, especially antioxidant content. Despite of its high potential, local knowledge and applicable tecgnology needed to isolate the precious content are still lacking. This study aimed to develop an effective method to get the highest possible yield of high quality extract from the Phaleria macrocarpa fruit. The method chosen was a non-destructive separation using liquid-solid extraction (leaching) operated in a continous mode by percolation method. Fruits after some pretreatment were extracted using ethanol 70% v /v with the variation of 4 extraction conditions namely: temperature (26 to 52 oC), the ratio of bed length to column diameter (4 to 6), particle diameter (0,22 to 0,90 cm), and solvent flow rate( 5 to 10 mL/min) . The experimental design used was Response Surface Method-Box-Behnken design with 3 center points. The condition of extraction were varied in order to optimize % yield and DPPH equivalent. The optimum condition were found at temperature 39 oC; lb/dk 4; particle diameter 0,22 cm; solvent flow rate 10 mL/min with 93,25% yield and DPPH equivalent of 3,21 μmol DPPH/mg crude extract.
The Effect of F:S Ratio, Temperature, Particle Diameter, and Mixing Speed in The Dispersive Contact Batch Extraction of Phaleria macrocarpa Fruit Using 70%-v Ethanol Solvent Susiana Prasetyo S
Prosiding Seminar Nasional Teknik Kimia "Kejuangan" 2015: Prosiding SNTKK 2015
Publisher : Seminar Nasional Teknik Kimia Kejuangan

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Phaleria macrocarpa is a native Indonesian plant from Papua, which has a high content of antioxidant and antimicrobial bioactive compounds. Therefore, it offers a very high potential to be utilized as the raw material for food preservative and supplement, as well as herbal medicine. This study was conducted for the development of the extraction and separation technology to obtain the useful bioactive compounds within a high quality extract from Phaleria macrocarpa fruit. Solid-liquid extraction was performed by dispersive contact using 70%-v ethanol in a 2 L batch extractor. The extraction process involved some variations of the process variables, namely F:S ratio (0.025 – 0.05 g/mL), temperature (28 - 52°C), particle effective diameter (0.22 – 0.90 cm), and mixing speed (140 – 400 rpm). The experimental series were carried out by using a Box-Behnken design with 3 center points, in order to comprehend both responses of yield oleoresin time profile and antioxidant activity (as DPPH equivalent). The experiments by varying those process variables could be used to estimate the volumetric mass transfer coefficient (kLa) using macroscopic balance based on a lumped model. The extraction optimum conditions were found at F:S ratio of 0.03 g/mL; temperature at 28°C; mixing speed 400 rpm; and particle diameter of 0.76 cm under which the highest yield of 94.56% , DPPH equivalent of 3.7364 μmol DPPH/mg crude extract, and mass transfer coefficient of 0.0192 s-1 were obtained.
The Pre-chromatography Purification of Crude Oleoresin of Phaleria Macrocarpa Fruit Extracts by Using 70%-v/v Ethanol Susiana Prasetyo S
Prosiding Seminar Nasional Teknik Kimia "Kejuangan" 2015: Prosiding SNTKK 2015
Publisher : Seminar Nasional Teknik Kimia Kejuangan

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Abstract

Phaleria macrocarpa is a native Indonesian plant which originated from Papua. The fruit posseses a high efficacy for health benefits due to the abundant content of antioxidant compounds, such as flavonoids, tanins, alkaloids, terpenoids and saponins. This research objective was to separate the various bioactive compounds in the extract (crude oleoresin) of the Phaleria macrocarpa fruit into several fractions, prior to a HPLC fraction collector, by using several selective solvents. The separation process utilized a liquid-liquid extraction method by an oscillation equipment for 1-2 hours. The extractions were carried out using F : S ratio of 1:1, at room temperature with 70%-v/v ethanol. The crude extract of each extract and raffinate was analyzed quantitatively. The quantitative analysis methods used were Obadoni and Ochuko for saponins test, n-hekxane separation for terpenoids test, and aluminium ion for flavonoids test. Folin-Ciocalteu and Sreevidya and Mehrotra methods were utilized for tannins and alkaloids tests, respectively. Meanwhile, the antioxidant activity was measured by DPPH method. The experimental results showed that the hexane fraction (first partition) was able to extract the resin component and selective enough to extract terpenoid groups (steroids) with Kd = 9.5960. The second partition, using chloroform solvent was not successful because of all the phytochemical components were evenly distributed in both fractions. Meanwhile, n-butanol fraction (third partition) selectively extract a large amount of flavonoid groups with Kd = 5.4880 but none of saponin was detected in this fraction. Flavonoid groups was found as the dominant component in the fruit extract contributed to the high antioxidant activity.
Isolasi Alginat Rumput Laut Coklat (Sargassum sp.) menggunakan Jalur Kalsium Alginat Susiana Prasetyo S; Olivia Juliani; Asaf Kleopas Sugih
Prosiding Seminar Nasional Teknik Kimia "Kejuangan" 2017: PROSIDING SNTKK
Publisher : Seminar Nasional Teknik Kimia Kejuangan

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Abstract

Alginate has been widely used in industrial field because of its natural properties as gelling agent. Brownseaweed, especially Sargassum and Turbinaria as the alginate sources are widely grown in Indonesia; butunfortunately there has yet to be any alginate industry in Indonesia. Alginate content in Sargassum isconsidered quite large, about 35%, whereas alginate content in Turbinaria is only around 20-25%. In thisresearch, sodium alginate was isolated from dried brown seaweed (Sargassum sp.) which first passedthrough the acid treatment using 0,5%-b/v HCl and alkaline treatment using 0,5%-b/v NaOH. Extraction wasdone in batch, using 2%-b/v Na2CO3 solvent. The chosen post-treatment method was through the Ca-alginatepath with experimental design Reponse Surface Methods-Central Composite Design with 5 center point. Thevaried variable was the concentration of CaCl2 (0,11 – 2,09 M) and the ratio of CaCl2 solution/alginateextract (0,48 – 4,02 g/g). The result showed that higher concentration of CaCl2 increased the yield, viscosity,and ash content. As the ratio of CaCl2/alginate extract got higher, the viscosity had the tendency to decreaseand the ash content increased. Yield of the obtained sodium alginate powder was 6,95 -30,7%; 1,48 – 11,85cP viscosity; ash content about 18,46 – 52,65%; and water content around 6,14 – 8,32%. The optimumcondition was obtained at CaCl2 concentration of 1,02 M and the ratio of CaCl2/alginate extract about 2,01g/g with 27,72% yield, 11,38 cP viscosity, 19,56% ash content, and 6,14-8,32% water content of sodiumalginate.
Co-Authors A Prima K A Prima Prima Anastasia Prima Kristijarti Andranyssa V M Rumondor Angelica Jennifer Asaf K Sugih Asaf Kleopas Sugih Deiza, Muhammad A Felicia M K Haryanto Felicia Yosephine Felicia Yosephine Henny Sunjaya Hudaya, Tedi Kristianto, Hans Loanda, Jordi Mariskha Tri Adithia, Mariskha Tri Nurmawan, Safri F Olivia Juliani Ramadhany, Putri Ratna Frida Susanti Soetedjo, Jenny Novianti M Tan, Daniel V Vincentius Yohanes Yanuar