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All Journal Jurnal Teknologi & Industri Hasil Pertanian

Murhadi Murhadi

Staf Pengajar Jurusan Teknologi Industri Pertanian Fakultas Pertanian Universitas Lampung

Author-ID : 3540864

Agriculture, Biological Sciences & Forestry Computer Science & IT Industrial & Manufacturing Engineering

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PENGARUH NISBAH TOTAL ETANOL – PKO DAN WAKTU REAKSI TERHADAP RENDEMEN DAN AKTIVITAS ANTIBAKTERI PRODUK ETANOLISIS MINYAK INTI SAWIT (PKO) Mairisa Lestari; Murhadi Murhadi
Jurnal Teknologi & Industri Hasil Pertanian Vol 13, No 2 (2008): Jurnal Teknologi & Industri Hasil Pertanian
Publisher : Teknologi Hasil Pertanian Fakultas Pertanian Universitas Lampung

The purposes of this work were to find the effects of ethanol-PKO ratio and reaction time on the yield and antibacterial activities of palm kernel oil ethanolysis products. The treatments were ratios of ethanolyisis mediums and duration of agitation. The medium treatments were ethanol 96% (NaOH 1%; w/w of oil) solution and PKO at ratio of 0,4; 0,7; 1,0; 1,3 and 1,6 (v/w) and the duration of agitation on a shaker at 300 rpm for 4, 6, 8, and 10 minutes. Antibacterial activities were tested using Staphylococcus aureus and Escherichia coli. The results showed ethanol-PKO ratio at 1,6 (v/w) effectively increased the yield of ethanolysis products for 4 up to 10 min, while the treatments at ratio of 0,4; 0,7; 1,0 (v/w) did not affect significantly, and at ratio of 1,3 (v/w) was optimum for 4 min. Ethanolysis products and their fractions for all treatments had antibacterial activities toward two bacteria (E. coli and S. aureus) with the average diameter of zone inhibition was 5,38 mm/15 mg. The optimum ethanol-PKO ratio and time of reaction were 1,3 (v/w) and 10 minutes to produce ethanolysis products, primarily for fraction 1 (uper layer after fractionation). Keywords: Antibacterial activities, ethanolysis, Palm Kernel Oil

KAJIAN LAMA REAKSI TERHADAP RENDEMEN MASSA DAN AKTIVITAS ANTIMIKROBA PRODUK GLISEROLISIS Havita Dwi Anggasari; Murhadi Murhadi
Jurnal Teknologi & Industri Hasil Pertanian Vol 14, No 1 (2009): Jurnal Teknologi & Industri Hasil Pertanian
Publisher : Teknologi Hasil Pertanian Fakultas Pertanian Universitas Lampung

The study was conducted to determine the effect of glycerolysis reaction time (48, 72, 96, 120 and 144 hours) on yield and antimicrobial activity of glycerolysis products from palm kernel oil (PKO) using rice bran as biocatalyst. The result showed that fractionation of glycerolysis products at all reaction time levels were yielding fraction A (61,8%) that higher than fraction B (21,8%) and fraction C (16,3%). Generally, all fractions (A, B and C) from all treatments have antimicrobial activity. Average of inhibition zone diameter (d) values of fraction C for all reaction time levels against S. aureus, B. subtilis, E. coli, and S. cerevisiae, i.e. 7,66; 6,81; 1,33; and 9,09 mm/6 mg fraction, respectively. Antimicrobial activity of fraction C was higher than fraction A and B, especially against S. aureus, B. subtilis, and S. cerevisiae at 72 and 96 h. of reaction times. Escherichia coli is more resistant bacteria to all fractions than another microbes in this study. All fractions from all reaction time levels be able to reduce of total mold (A. orizae) colony with each average percentage of 79,6 (fraction A); 81,0 (fraction B); and 78,2% (fraction C). Keywords: Glycerolysis, PKO, fraction, antimicrobial activity, resistant.

SENYAWA DAN AKTIVITAS ANTIMIKROBA GOLONGAN ASAM LEMAK DAN ESTERNYA DARI TANAMAN Murhadi Murhadi
Jurnal Teknologi & Industri Hasil Pertanian Vol 14, No 1 (2009): Jurnal Teknologi & Industri Hasil Pertanian
Publisher : Teknologi Hasil Pertanian Fakultas Pertanian Universitas Lampung

Problems in food preservation have grown to be more complex as new food products are continuously introduced onto the market. They require longer shelf life and greater assurance of protection from microbial spoilage. There are several chemicals that can be used as antimicrobial agents. For instance, acetic acid and sulfur dioxide are widely used as food preservatives. However, these chemicals required caution in handling since they are corrosive and their vapors can irritate the eyes and respiratory tract. Although conventional synthetic antimicrobial agents have excellent activities, their safety in human body has been considered doubtful. Since it is known that antimicrobial agents are contained in natural materials, many research studies have been carried out to improve the shelf stability of foods and cosmetics using natural antimicrobial agents. Many naturally occurring compounds found in edible and medicine plants, herbs, and spices have been shown to possess antimicrobial function and could serve as a source of antimicrobial against food pathogens. Recently the interest in the biological activities of plant extracts has been rekindled and has been the subject of intense scientific investigation. Fatty acid and its ester forms as monoglyceride and or diglyceride of plant materials, especially from palm kernel oil and coconut oil such as lauric acid (12:0), miristic acid (14:0) and capric acid (10:0) have antimicrobial function. The antimicrobial activities of monolaurin, monomiristin and or monocaprin and its diglyceride’s forms are well recognized and showed a wide spectrum of antimicrobial activities toward several microbes. Keywords: Fatty acids, monolaurin, monomiristin, monocaprin, antimicrobial activities

KAJIAN AKTIVITAS ANTIBAKTERI PRODUK ETANOLISIS DARI CAMPURAN MINYAK INTI SAWIT (Elaeis quineensis Jacq.) DAN MINYAK BIJI MENGKUDU (Morinda citrifolia L.) Murhadi Murhadi; Suharyono AS Suharyono AS
Jurnal Teknologi & Industri Hasil Pertanian Vol 13, No 2 (2008): Jurnal Teknologi & Industri Hasil Pertanian
Publisher : Teknologi Hasil Pertanian Fakultas Pertanian Universitas Lampung

The objective of this research was to measure antibacterial activities of ethanolysis products that produced through ethanolysis reaction of mixture of palm kernel oil (PKO) and Morinda seeds oil (25:1 and 50:1, w/w) in ethanol solution contained 1% NaOH (v/w based on oil weight) at ratio of 5 : 6 (b/v) and shaken on a shaker (300 rpm, room temp.) for 3, 6, 9 and 12 minutes. Fractionation of ethanolysis products using absolute ethanol (1:3; v/v) and centrifuged at 4.000 rpm for 30 min, yielding Fraction 1 (upper layer) and Fraction 2 (lower layer). Generally, all ethanolysis products showed antibacterial activities against four test bacteria (E. coli, S. aureus, B. cereus and S. enteritidis) at range of d values (mm) from 0,93 (B. cereus; 3 min) up to 3,87 (E. coli; 9 min). Fraction 1 showed the highest antibacterial activity with d values (mm) from 1,20 (B. cereus; 9 min) up to 5,63 (E. coli; 9 min), while Fraction 2 for all treatments had no antibacterial activity, relativelly. Ethanolysis products and its fraction (Fraction 1) were resulted from ethanolysis reaction for 6 and 9 min showed higher antibacterial activities than those for 3 and 12 min. Keywords: Antibacterial activities, ethanolysis product, Morinda seeds oil, PKO

Co-Authors Havita Dwi Anggasari Mairisa Lestari Suharyono AS Suharyono AS

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