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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Hortikultura Indonesia (JHI) Menara Perkebunan BIOVALENTIA: Biological Research Journal Jurnal Pusat Inovasi Masyarakat
Megayani Sri Rahayu
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (IPB University)
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Immunology & microbiology Medicine & Pharmacology Social Sciences

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Journal : Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)

 1 
Seleksi Pendonor Serbuk Sari Sifat Kuantitas Endosperma Skor Tinggi pada Kelapa dalam Kopyor Rahayu, Megayani Sri; Setiawan, Asep; Maskromo, Ismail; Purwito, Agus; Sudarsono, dan
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 47 No. 1 (2019): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (436.174 KB) | DOI: 10.24831/jai.v47i1.21116

Abstract

Endosperm quantity in kopyor coconut determines the quality of kopyor coconut. However, data of the best parental based on their progeny test is unavailable yet. In this study, we employed polycross approach and SSR molecular marker to estimate general combining ability of the best pollen contributor for the breeding purpose of this trait. Totally 151 kopyor coconuts were collected from the naturally open-pollinated population of kopyor Tall coconut Agom Lampung. The harvested nuts were rated for their endosperm quantity (scored 1-9). The analysis identified 31 paternal trees of the 53 progenies. The TJ-185 with the highest GCA has been considered as a promising paternal tree for development of high endosperm quantity of kopyor coconut.Keywords: combining ability, molecular marker, paternal trees, polycross, tall coconut
Regeneration of Raja (Musa AAB Group) and Kepok (Musa ABB Group) bananas on various stages of in vitro culture Nazihah, Shafira Puti; Rahayu, Megayani Sri; Armini Wiendi, Ni Made
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 51 No. 1 (2023): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (394.906 KB) | DOI: 10.24831/ija.v51i1.46196

Abstract

Banana group can be divided into two groups based on their method of its utilization. Banana group that can be eaten immediately after ripening is called "dessert banana", e.g., Raja, while the group that needs particular processing before consumption is called "plantain banana", e.g., Kepok. This study aimed to compare the growth of Raja banana with Kepok plantain through experiments at three stages of the in vitro culture media: the shoot initiation, shoot multiplication, and plantlet acclimatization, also to compare the growth of Raja bananas with Kepok plantains in response to cytokinins BAP and TDZ at shoot multiplication stage. Planting material was in the form of rhizomes that was prepared with the same size. The experiment was performed using a completely randomized design. Results showed that Kepok plantain could adapt faster to the media condition than Raja banana; the phenomenon was evident in the growing speed of Kepok during shoot initiation stage. The proportion of the B genome did not show a direct effect on shoot induction at the multiplication stage or enlargement and organ formation at the acclimatization stage. Shoot induction at the multiplication stage depended more on the composition of the media used. The combination of BAP 3 mg L-1 and TDZ 0.01 mg L-1 in MS media produced the best shoot induction rate, and TDZ 0.01 mg L-1 in MS media had the highest shoot elongation rate. Keywords: banana, BAP, genome, PGR, plantain, TDZ
Callus formation response from immature male flower explant of plantain banana (Musa acuminata x Musa balbisiana cv. Kepok) treated by 2,4-D and BAP Dhiya Nabilla Ardhani; Maharijaya, Awang; Megayani Sri Rahayu
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 52 No. 1 (2024): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v52i1.49008

Abstract

Callus induction is an important step in indirect somatic embryogenesis. The aim of this study was to obtain an optimal medium for the callus formation of plantain bananas (cv. Kepok). The research was conducted from May to August 2022 at the Tissue Culture Laboratory of PT. ITCI Kartika Utama, Penajam Paser Utara District, East Kalimantan. The explant used an immature male flower of plantain banana (cv. Kepok). The experiment used a completely randomized design with two factors concentration of 2,4-D (1,2 and 4 ppm) and BAP (5, 10, and 15 ppm). The appearance of callus was monitored daily until it developed explants. On the final observation day, the percentage of explants with callus, as well as the color and texture of the callus, were assessed. The research findings suggest that the optimal medium for inducing callus in male banana flower explants was MS medium supplemented with 2 ppm 2,4-D and 5 ppm BAP. This medium resulted in calluses that exhibited a yellowish-white color and a compact texture. Keywords: callus morphology; genetic improvement; plant growth regulator; tissue culture; triploid banana
Co-Authors , Krisantini Afifah Farida Jufri Agus Purwito Ananda Diah Oktaviani Andriani, Ratna Asep Setiawan Asih, Ni Putu Sri Awang Maharijaya Bambang Suhartawan Daawia, Daawia Dede Kismantoro Dhiya Nabilla Ardhani Diny Dinarti Efendi, Darda Erna Siaga, Erna Erna Sinaga Fitriyah Nurul Flowrentyka Ferona Haloho Frederico Tunggal Gina Rahma Utami Halimah Widyaningrum Haris Aprianto Setiawan Hasrat Enggal Prayogi Iskandar Lubis Ismail Maskromo Juang Gema Kartika Jufri, Afifah Farida Krisantini Matra, Deden D. Maudy Susanti Nazihah, Shafira Puti Ni Made Armini Wiendi Ni Made Armini Wiendi, Ni Made Novita Niken Putri Ulayyah Nurhalim Nyssa Azaria Dewani Ramdani, Adrian Setia Kurniawan Setiawan, Asep S Setiowati, Retno Diah Sri Wening Sudarsono Sudarsono, dan Titiek S. Yuliani Tri Rini Nuringtyas Utami, Gina Rahma Visasti , Khurul