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All Journal Jurnal Sain Veteriner Jurnal Veteriner Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Jurnal Kedokteran Hewan
Abdul Samik
Departemen Reproduksi Veteriner Fakultas Kedokteran Hewan Universitas Airlangga Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Journal : Jurnal Kedokteran Hewan

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Characterization of Pregnancy Specific Protein B (PSPB) from Placenta Foetalis (Cotyledon) Friesian Holstein Cows Abdul Samik
Jurnal Kedokteran Hewan Vol 2, No 1 (2008): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (329.971 KB) | DOI: 10.21157/j.ked.hewan.v2i1.9779

Abstract

This research was aimed to find out a substance that was useful in early pregnancy diagnosis of Friesian Holstein cows. Pregnancy Specific Protein B (PSPB) was isolated, purified and partially characterized from cotyledon Friesian Holstein cows. Characterization of PSPB protein was conducted using SDS-PAGE and Western Blot. Antisera were developed against PSPB and by immunohistochemical techniques the protein was localized to the binucleated cells of the cotyledon. The estimated molecular size of Friesian Holstein cows PSPB was 59.88 kDa with the concentration of PSPB protein in cotyledon was 6480 ng/ml. PSPB protein could induce anti-PSPB antibody with the value of optical density (OD) were 0.179±0.0102 (before immunization); 1.466±0.3288 (3rd week after immunization); 1.936±0.4754 (1st week after booster;) and 2.256±0.4842 (2nd week after booster).
Co-Authors Afiff , Usamah Anriansyah Renggaman Dea Indriani Astuti Dedi Rahmat Setiadi Hana Eliyani Imam Mustofa Intan Purwa Dewanti Kusnoto Kusnoto Lucia Tri Suwanti, Lucia Tri Mafatichul Firdaus Mufasirin MUHAMMAD AGIL Muhammad Thohawi Elziyad Purnama Ngakan Made Rai Widjaja Poedji Hastutiek Prastiya, Ragil Angga Raden Tatang Santanu Adikara Saputro, Amung Logam Soeharsono Soeharsono Surya Agus Prihatno Tatik Hernawati Tri Wahyu Suprayogi Warda Nafalizza Efendi Yeni Dhamayanti Yosua Kristian Adi Yudit Oktanella, Yudit