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All Journal Jurnal Sain Veteriner Jurnal Veteriner Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Jurnal Kedokteran Hewan
Abdul Samik
Departemen Reproduksi Veteriner Fakultas Kedokteran Hewan Universitas Airlangga Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Characterization of Pregnancy Specific Protein B (PSPB) from Placenta Foetalis (Cotyledon) Friesian Holstein Cows Abdul Samik
Jurnal Kedokteran Hewan Vol 2, No 1 (2008): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v2i1.9779

Abstract

This research was aimed to find out a substance that was useful in early pregnancy diagnosis of Friesian Holstein cows. Pregnancy Specific Protein B (PSPB) was isolated, purified and partially characterized from cotyledon Friesian Holstein cows. Characterization of PSPB protein was conducted using SDS-PAGE and Western Blot. Antisera were developed against PSPB and by immunohistochemical techniques the protein was localized to the binucleated cells of the cotyledon. The estimated molecular size of Friesian Holstein cows PSPB was 59.88 kDa with the concentration of PSPB protein in cotyledon was 6480 ng/ml. PSPB protein could induce anti-PSPB antibody with the value of optical density (OD) were 0.179±0.0102 (before immunization); 1.466±0.3288 (3rd week after immunization); 1.936±0.4754 (1st week after booster;) and 2.256±0.4842 (2nd week after booster).
Co-Authors Adikara, Tatang Santanu Afiff , Usamah Anang Yusuf Annisa Lita Triagil Anriansyah Renggaman Arda Wahyu Setyadi Bimo Aksono Boedi Setiawan BUDI UTOMO Dea Indriani Astuti Dedi Rahmat Setiadi Eka Pramyrtha Hestianah Fierda Kabayo Hana Eliyani Hani Plumeriastuti Hanifah Muslimah Ananda Hermin Ratnani Herry Agoes Hermadi Herry Agus Hermadi Imam Mustofa Indah Norma Triana Intan Purwa Dewanti Ira Sari Yudaniayanti Ismudiono Ismudiono Iwan Sahrial Hamid Koesnoto Soepranianondo Kusnoto Kusnoto Lucia Tri Suwanti, Lucia Tri Mafatichul Firdaus Mas'ud Hariadi Mas’ud Hariadi Mas’ud Hariadi Mufasirin MUHAMMAD AGIL Muhammad Billy Ferdiansyah Muhammad Thohawi Elziyad Purnama Nadia Yohana Nenny Harijani Ngakan Made Rai Widjaja Norma Amalia Ulfah Nove Hidajati Novia Alfi Masruro Poedji Hastutiek Prastiya, Ragil Angga Pudji Srianto R.T.S Adikara R.T.S Adikara Ricko Nurdianto S. Saputro, Amung Logam Sarah Azura Satya Alysa Cahya Puspita Soeharsono Soeharsono Soeharsono Soeharsono Sri Hidanah Sri Hidanah Sri Mulyati Sri Pantja Madyawati Suherni Susilowati Surya Agus Prihatno Suzanita Utama Tatik Hernawati Tita Damayanti Lestari Tjuk Imam Restiadi Tri Wahyu Suprayogi Trilas Sardjito Warda Nafalizza Efendi Widiyatno, Thomas Valentinus Widodo, Oky Setyo Wurlina, W Yeni Dhamayanti Yosua Kristian Adi Yuanara Augusta Rahmat Adikara Yudit Oktanella, Yudit