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All Journal HAYATI Journal of Biosciences Microbiology Indonesia Jurnal Matematika & Sains Pharmauho: Jurnal Farmasi, Sains, dan Kesehatan The Indonesian Biomedical Journal Jurnal Farmasi Indonesia YARSI Medical Journal
DEBBIE SOEFIE RETNONINGRUM
School of Pharmacy, Institut Teknologi Bandung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Immunology & microbiology Mathematics Medicine & Pharmacology Public Health

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Journal : Jurnal Farmasi Indonesia

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Pengaruh Superoksida Dismutase Rekombinan Staphylococcus equorum Terhadap Viabilitas Sel dan Deposisi Kolagen Pada Sel Fibroblas 3T3 Yang Dipaparkan UVA Ana Indrayati; Sukmadaja Asyarie; Tri Suciati; Debbie Soefie Retnoningrum
Jurnal Farmasi Indonesia Vol 13 No 1 (2016): Jurnal Farmasi Indonesia
Publisher : Fakultas Farmasi Universitas Setia Budi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (80.827 KB) | DOI: 10.31001/jfi.v13i1.94

Abstract

UVA is the main external factors causing skin aging. UVA increase matrix metalloproteinase (MMP) synthesis that degrade collagen indirectly through reactive oxygen species. Superoksida dismutase (SOD) catalyzes the conversion of superoxide anions to hydrogen peroxide and oxygen. SOD can be used as a cosmetic’s component to prevent skin aging. The objective of this research to determine rSOD S. equorum ability to increase collagen deposition using fibroblast 3T3 against UVA. The research was initiated by the confirmation of E. coli BL21(DE3) carrying pJExpress®414-sod. rSOD was overproduced in E. coli BL21(DE3) by IPTG induction to a final concentration of 1 mM for 4 hours at 37oC. rSOD purification was done using an Ni-NTA affinity column with gradient imidazole concentrations for elution. Various concentrations of rSOD was added to fibroblast 3T3 cell culture after UVA irradiation to determine its role in collagen deposition. The effect of rSOD was analyzed by fibroblast viability using Alamar blue and collagen deposition with picro sirius red. The results showed that fibroblast cell viability exposed with UVA for 45 minutes in the presence of 4, 8, and 16 unit rSOD was not significantly affected, however, the collagen deposition was significantly increased about 3.02%; 20.03% and 35.75% respectively compared to cell without rSOD. This result indicates that rSOD is a good candidate for an anti photoaging agent.
Co-Authors ADAM REZA GANJARA Ana Indrayati ARTARINI, ANITA CATUR RIANI CHANDRA JINATA Chatib Warsa, Usman Dessy Natalia Dewi Riskha Nurmalasari Elfahmi Elfahmi, Elfahmi Elvi Restiawaty Endang Purwantini Enny Ratnaningsih ERNAWATI ARIFIN GIRI-RACHMAN Ernawati Arifin Giri-Rachman Fatmawaty Badaruddin Imam Supardi LARAS AJENG PITAYU Muhaimin Muhaimin Neni Nurainy Novia Syari Intan Oei Ban Liang Patricia Gita Naully Permanasari, Silmi Citra Putri Ayu Fajar Raymond R. Tjandrawinata ROGA FLORIDA KEMBAREN RUKMAN HERTADI Sukmadaja Asyarie Sumirtapura, Yeyet Cahyati Tan, Marselina Irasonia Tina Rostinawati Tri Suciati VALENTINA YURINA VALENTINA YURINA Wardono Niloperbowo Wojciechowska, Gladys Emmanuella Putri