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All Journal JCRS (Journal of Community Research and Service) Jurnal Mandala Pharmacon Indonesia Hydrogen: Jurnal Kependidikan Kimia Abdimas Indonesian Journal Jurnal Pengabdian Masyarakat Panacea Journal of Pharmaceuticals and Natural Sciences al Kimiya : Jurnal Ilmu Kimia dan Terapan Jurnal Sains dan Kesehatan Jurnal Riseta Naturafarm Jurnal Pendidikan MIPA
Laode Rijai
Fakultas Farmasi Universitas Mulawarman
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary

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Journal : Jurnal Riseta Naturafarm

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Secondary Metabolites and Cytotoxicity of Kitolod Leaf Extract (Hippobroma longiflora (L.) G. Don to Shrimp Larvae (Artemia salina Leach): Metabolit Sekunder dan Sitotoksisitas Ekstrak Daun Kitolod (Hippobroma longiflora (L.)) terhadap Larva Udang (Artemia salina Leach) Ibrahim, Arsyik; Bulan, Adisty Sindi; Ramadhan, M. Rahmad; Bone, Mahfuzun; Rija'i, Hifdzur Rashif; Rusman, Arman; Arifuddin, M.; Junaidin, Junaidin; Rijai, Laode
Jurnal Riseta Naturafarm Vol. 2 No. 1 (2025): Jurnal Riseta Naturafarm
Publisher : B-Creta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70392/jrn.v2i1.3342

Abstract

One of the plants used by the people of East Kalimantan, especially the people of West Kutai, as a medicinal plant is Kitolod (Hippobroma longiflora L.). The plant parts used are the leaves and flowers. The secondary metabolites contained in Kitolod leaves are alkaloids, saponins, flavonoids and polyphenols. The aim of this research is to identify secondary metabolite groups and test the cytotoxicity of Kitolod leaf extracts and fractions. Secondary metabolites are identified qualitatively using various chemical reagents and metabolites characterized by the presence or absence of color changes or the presence of sediment or foam. Cytotoxicity testing was carried out using the Brine Shrimp Lethality Test (BSLT) method. The data research was analyzed using the Reed and Muench method to determine the Lethality Concentration 50% value. The results of research on the metabolite content of ethanol extract, fractions: n-hexane, ethyl acetate and n-butanol contain alkaloid, flavonoids and phenolic/polyphenols compounds. The bioactivity test results for the ethanol extract had an LC50 value of 592.93 ppm, the n-hexane fractions of 582.77 ppm, the ethyl acetate fraction of 531.37 ppm, and the n-butanol fraction of 119.48 ppm. These results indicate that the n-butanol fraction of Kitolod leaves has stronger cytotoxic activity than other extracts. .
Isolation and Identification of Lactic Acid Bacteria from the Washing Water of Mayas Rice, a Traditional Mountain Rice from East Kalimantan: Isolasi Dan Identifikasi Bakteri Asam Laktat Dari Air Cucian Beras Gunung (Beras Mayas) Khas Kalimantan Timur Amanda, Rezti; Rijai, Laode; Arifuddin, Muhammad
Jurnal Riseta Naturafarm Vol. 2 No. 2 (2025): Jurnal Riseta Naturafarm
Publisher : B-Creta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70392/jrn.v2i2.96104

Abstract

Rice washing water contains carbohydrates, vitamins, and minerals that serve as potential substrates for the growth of Lactic Acid Bacteria (LAB). This study aims to isolate and characterize LAB from the traditional East Kalimantan rice variety Beras Mayas. LAB isolation was performed using De Man Rogosa and Sharpe (MRS) Agar, followed by successive purification to obtain pure cultures. Identification of the isolates involved macroscopic and microscopic observations, Gram staining, and a series of biochemical assays. Three distinct LAB isolates, designated A, B, and C, were obtained. Isolates A and B exhibited circular colonies with yellowish-white pigmentation and undulate margins, suggesting affiliation with the genus Lactobacillus. Isolate C formed circular white colonies with entire margins, indicating a possible relationship with the genus Streptococcus. Biochemical testing revealed that all three isolates were negative for catalase, gelatin hydrolysis, motility, and indole production. In the Triple Sugar Iron Agar (TSIA) test, all isolates fermented glucose, lactose, and sucrose, producing yellow coloration in both the slant and butt portions of the medium. These results suggest that the isolates are homofermentative LAB.
Co-Authors Agus Setiawaty Almeida, Maria Amanda, Rezti Anggreini, Putri Arifian, Hanggara Arifuddin, M Arifuddin, Muhammad Arum, Alvionita Puspa Badawi, Satriani Baso Didik Hikmawan Bone, Mahfuzun Bulan, Adisty Sindi Daniel Happy Putra Fadhil, Andi Raihan Fahrul Rozi Farah Erika Farah, Harra Ismi Febrina, Lizma Herliani Herliani Herman Herman Herman Hernawati, Desy Hikmawan, Baso Didik Ibrahim, Arsyik Islamudin Ahmad Islamudin Ahmad Jeremia Giawa, Agusto Junaidin, Junaidin Kadir, Nurdianah Abdul Kama Tasawa, Gymnastiyar Lestari, Wahyu Yunita M. Arifuddin, M. Mohd Azlan Nafiah, Mohd Azlan Muhammad Faisal Mus, Nurul Muhlisa Olivia Siregar, Vita Purwita Sari, Regina Rahmadani, Agung Ramadhan, M. Rahmad Rashif Rijai, Hifdzur Rezky Khairun Nisaa, Nur Rezqiyana, Gita Anna Ridhotulloh, Yusuf Isro Rija'i, Hifdzur Rashif Rijai, Hifdzur Rashif Rija’i, Hifdzur Rashif RIKI RIKI, RIKI Rusli, Rolan Rusman, Arman Salam, Supriatno Samsul, Erwin Saputri, Mitha Sinthary, Venna Sita, Annisa Aulia Siti Hardiyanti Sugeng, Santoso Sukemi Sukemi Supriatno Tasya, Indriana Usman Usman Wirhanuddin, Wirhanuddin Yazid, Muhammad Ilfan