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All Journal Microbiology Indonesia Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Medical Journal of Indonesia Dental Journal (Majalah Kedokteran Gigi) Universa Medicina Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) Eksakta : Berkala Ilmiah Bidang MIPA Jurnal Biomedika dan Kesehatan
Yeva Rosana
Department Of Microbiology, Faculty Of Medicine, Universitas Indonesia, Jakarta 10430
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Public Health

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Journal : Microbiology Indonesia

 1 
Modified Slide Culture Method for Faster and Easier Identification of Dermatophytes YEVA ROSANA; TETSUHIRO MATSUZAWA; TOHRU GONOI; ANIS KARUNIAWATI
Microbiology Indonesia Vol. 8 No. 3 (2014): September 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1069.613 KB) | DOI: 10.5454/mi.8.3.7

Abstract

Basic slide culture as a morphological identification was known as the most common method for the identification of pathogenic mold fungi. This method preserved the morphological features relatively undisturbed compared with adhesive tape preparations. However, it was necessary to modify method of basic slide culture to improve its usability and shorten the time it needed to identify mold fungi. There were four kinds of method carried out in this study; two kinds of modified slide culture, one kind of direct culture on slant agar plate, and a basic slide culture for identifying mold fungi, which result would be compared with each other. These four methods were tested to 4 species of dermatophytes which were known as mold fungi that could infect skin, hair, and nails in human; those were Trichophyton mentagrophytes, Microsporum canis, Microsporum gypseum, and Epidermophyton floccosum. Result of this study showed that both modified slide culture and direct culture on slant agar plate could visualize the structure of dermatophytes faster than basic slide culture method. These methods were also easier to prepare compared to basic culture method. Conclusion of this study showed that basic slide culture method needed to be modified for better identification of mold fungi.
Comparison of Microbial Pattern Causing Urinary Tract Infection in Female Out- and Hospitalized Patients in Jakarta YEVA ROSANA; DWIANA OCVIYANTI; ANIS KARUNIAWATI; SYADZA RHIZKY PUTRI AKHMAD
Microbiology Indonesia Vol. 10 No. 1 (2016): March 2016
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (493.251 KB) | DOI: 10.5454/mi.10.1.5

Abstract

Urinary Tract Infection (UTI) is an infection in any part of the urinary system. Women are 3 times more likely to have UTI than men. The UTI accounts for 15% infection cases in outpatients and 24% cases in hospitalized patients. Although the most common cause of UTI is certain bacteria, but it was not easy to choose the appropriate antimicrobial therapy. Strategy for choosing empiric antimicrobial treatments for UTI in female out- and hospitalized patients should be based on the pattern of the causative organisms. The aim of this study was to understand the microbial pattern causing UTI in female out- and hospitalized patients in Jakarta. The UTI -1 causative microorganisms were obtained from urine culture containing 100,000 cfu/mL . Twenty nine microorganisms were found as the causative agents of UTI in 317 pregnant women who came to six Community Health Centres (Puskesmas) in Jakarta: Makassar; Pulogadung, Cakung, Pasar Rebo, Duren Sawit, and Kramat Jati for antenatal care. Twenty nine microorganisms were isolated from 114 urine samples of female hospitalized patients who were diagnosed of UTI. The samples were obtained from the Microbiology Laboratory Clinic of FKUI-RSCM. The most common microorganisms causing UTI in female out- and hospitalized patients were Gram negative bacteria. In female outpatients, Klebsiella sp was the most common causative bacteria (31%), followed by Escherichia coli (24.1%). In female hospitalized patients, Escherichia coli was the most common causative bacteria (30%), followed by Candida sp (24.1%) and Klebsiella pneumonia (6.8%). There was more variation in the pattern of UTI causative organisms in hospitalized female patients in comparison to that of the outpatients. Candida sp. was only found in hospitalized UTI patients but not in outpatients.
Co-Authors Abas, Ghina Mutiara Andi Yasmon Anggraini Suwarsono, Erike Anis Karuniawati Ariyani Kiranasari Bachti Alisjahbana Bachti Alisyahbana Basri A. Gani Beti E. Dewi Conny R. Tjampakasari Conny Tjampakasari Dick van Soolingen Dwiana Ocviyanti E. Risdiyani Efendi, Ida Effendi, Ida Fera Ibrahim Ferry Darmawan Fithriyah Fithriyah Gultom, Desy A. Gus Permana Subita Herliyana, Lina Ibnu Agus Ariyanto Ida Parwati Ika Ningsih Imaniar, Clara Krisandi, Grady Louisa Ivana Utami Mardiastuti H Wahid Pratiwi Pujilestari Sudarmono Prawoto Prawoto Rahmah Amran Reinout van Crevel Rela Febriani Retno Kadarsih S. Nilawati S. Sunnati Shanty Olivia Siti Aliyah Pradono SJATHA, FITHRIYAH Sri Rezeki SYADZA RHIZKY PUTRI AKHMAD T. M. Sudiro TETSUHIRO MATSUZAWA Tjahjani M. Sudiro TOHRU GONOI Wia Melia Wresti Indriatmi B. Makes