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All Journal JBIO: jurnal biosains (the journal of biosciences) Jurnal Peternakan (Jurnal of Animal Science) Community Development Journal: Jurnal Pengabdian Masyarakat Arwana: Jurnal Ilmiah Program Studi Perairan Devotion: Journal of Research and Community Service SEAS (Sustainable Environment Agricultural Science) Jurnal Kedokteran Hewan The International Journal of Tropical Veterinary and Biomedical Research Lentera

Wenny Novita Sari

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Author-ID : 373405

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary

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KOSAKONIA SP. PROTEOLYTIC BACTERIA ISOLATED FROM RUMEN AND RETICULUM OF ACEH CATTLE Safika Safika; Wenny Novita Sari; Gressha Vionalle Ademi; Ulfi Widi Arsih; Darmawi Darmawi
Jurnal Kedokteran Hewan Vol 14, No 2 (2020): June
Publisher : Universitas Syiah Kuala

The aim of this study was to identify proteolytic bacteria from the ruminal and reticulum fluids of aceh cattle based on the 16S rRNA gene. Samples used were ruminal and reticulum fluids of aceh cattle slaughtered in Abattoir of Aceh Besar. Samples were diluted and cultured into Skim Milk Agar medium at 39 C for 48 hours. The morphology of bacterial colonies growth in the medium was observed. Colonies resulted in the largest clear zone were isolated and used for Deoxyribonucleic Acid (DNA) isolation, 16S rRNA gene amplification and sequencing. Theresults showed that morphology of dominant colonies was yellowish white color, round shape, position on the agar surface. The results ofphylogenetic analysis of RS1 and ReS2 isolates isolated from rumen and reticulum fluids of aceh cattle respectively had a close familialrelationship and belonged to the bacterial group of Kosakonia. Sequence homology showed isolate RS1 and ReS2 are probably either newEnterobacteriaceae species or unconfirmed species. Halo zone produced by ruminal bacteria had a wider diameter (25 mm vs 20 mm) than thatcaused by reticulum bacteria). Based on the results, RS1 (bacterium in the rumen) and ReS2 (bacterium in the reticulum) belong to similar type, namely Kosakonia sp. with a proteolytic activity. Presumably, these bacteria originate from the rumen that enters the reticulum with degraded feed.

PERSENTASE BERAHI DAN KEBUNTINGAN KAMBING PERANAKAN ETTAWA (PE) SETELAH PEMBERIAN BEBERAPA HORMON PROSTAGLANDIN KOMERSIAL Hafizuddin Hafizuddin; Wenny Novita Sari; Tongku Nizwan Siregar; Hamdan Hamdan
Jurnal Kedokteran Hewan Vol 5, No 2 (2011): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui perbandingan efektivitas pemberian hormon prostaglandin komersial yang berbeda terhadap persentase berahi dan kebuntingan kambing peranakan ettawa. Kambing betina yang digunakan mempunyai kriteria umur 2,5-3,5 tahun, sehat, tidak bunting, minimal 2 bulan pasca partus, sudah pernah beranak, dan mempunyai bobot badan yang relatif sama. Di samping itu digunakan 2 ekor kambing jantan untuk membantu deteksi berahi. Hewan percobaan dibagi atas 3 kelompok perlakuan, masing-masing kelompok terdiri atas 5 ekor kambing. Kelompok perlakuan I diinjeksi dengan 2,5 ml Lutalyse (dinoprost tromethamine 5 mg/ml dan benzil alkohol 1,65%), kelompok perlakuan II diinjeksi dengan 0,5 ml Prostavet (etiproston 5 mg/2 ml dan ethylen dioxy 15 mg/2 ml), dan kelompok perlakuan III diinjeksi dengan 1,5 ml Capriglandin (dinoprost tromethamine 5,5 mg/ml dan benzil alkohol 12,0 mg/ml). Penyuntikan dilakukan 2 kali secara intramuskuler dengan interval 10 hari setelah penyuntikan pertama. Kambing-kambing yang memperlihatkan gejala berahi dikawinkan secara inseminasi buatan. Diagnosis kebuntingan dilakukan dengan menggunakan USG 30 hari setelah inseminasi. Data onset berahi dianalisis menggunakan analisis varian, sedangkan persentase berahi dan kebuntingan dianalisis secara deskriptif. Onset berahi ketiga kelompok perlakuan masing-masing adalah 40,8±0,57 36,0±0,57 dan 50,4±1,52 jam (P0,05). Persentase berahi pada ketiga kelompok adalah sama yakni 100%, sedangkan persentase kebuntingan pada kelompok I, II, dan III masing-masing adalah 100, 80, dan 60%. Dari hasil penelitian dapat disimpulkan bahwa onset dan persentase berahi tidak dipengaruhi oleh ketiga prostaglandin komersial yang berbeda tetapi berpengaruh terhadap persentase kebuntingan kambing PE.

KOSAKONIA SP. PROTEOLYTIC BACTERIA ISOLATED FROM RUMEN AND RETICULUM OF ACEH CATTLE Safika, Safika; Sari, Wenny Novita; Ademi, Gressha Vionalle; Arsih, Ulfi Widi; Darmawi, Darmawi
Jurnal Kedokteran Hewan Vol 14, No 2 (2020): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i2.16426

The aim of this study was to identify proteolytic bacteria from the ruminal and reticulum fluids of aceh cattle based on the 16S rRNA gene. Samples used were ruminal and reticulum fluids of aceh cattle slaughtered in Abattoir of Aceh Besar. Samples were diluted and cultured into Skim Milk Agar medium at 39 C for 48 hours. The morphology of bacterial colonies growth in the medium was observed. Colonies resulted in the largest clear zone were isolated and used for Deoxyribonucleic Acid (DNA) isolation, 16S rRNA gene amplification and sequencing. Theresults showed that morphology of dominant colonies was yellowish white color, round shape, position on the agar surface. The results ofphylogenetic analysis of RS1 and ReS2 isolates isolated from rumen and reticulum fluids of aceh cattle respectively had a close familialrelationship and belonged to the bacterial group of Kosakonia. Sequence homology showed isolate RS1 and ReS2 are probably either newEnterobacteriaceae species or unconfirmed species. Halo zone produced by ruminal bacteria had a wider diameter (25 mm vs 20 mm) than thatcaused by reticulum bacteria). Based on the results, RS1 (bacterium in the rumen) and ReS2 (bacterium in the reticulum) belong to similar type, namely Kosakonia sp. with a proteolytic activity. Presumably, these bacteria originate from the rumen that enters the reticulum with degraded feed.

PERSENTASE BERAHI DAN KEBUNTINGAN KAMBING PERANAKAN ETTAWA (PE) SETELAH PEMBERIAN BEBERAPA HORMON PROSTAGLANDIN KOMERSIAL Hafizuddin, Hafizuddin; Sari, Wenny Novita; Siregar, Tongku Nizwan; Hamdan, Hamdan
Jurnal Kedokteran Hewan Vol 5, No 2 (2011): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v5i2.366

Penelitian ini bertujuan mengetahui perbandingan efektivitas pemberian hormon prostaglandin komersial yang berbeda terhadap persentase berahi dan kebuntingan kambing peranakan ettawa. Kambing betina yang digunakanmempunyai kriteria umur 2,5-3,5 tahun, sehat, tidak bunting, minimal 2 bulan pasca partus, sudah pernah beranak, dan mempunyai bobot badan yang relatif sama. Di samping itu digunakan 2 ekor kambing jantan untuk membantu deteksi berahi. Hewan percobaan dibagi atas 3 kelompok perlakuan, masing-masing kelompok terdiri atas 5 ekor kambing. Kelompok perlakuan I diinjeksi dengan 2,5 ml Lutalyse (dinoprost tromethamine 5 mg/ml dan benzil alkohol 1,65%),kelompok perlakuan II diinjeksi dengan 0,5 ml Prostavet (etiproston 5 mg/2 ml dan ethylen dioxy 15 mg/2 ml), dan kelompok perlakuan III diinjeksi dengan 1,5 ml Capriglandin (dinoprost tromethamine 5,5 mg/ml dan benzil alkohol 12,0 mg/ml). Penyuntikan dilakukan 2 kali secara intramuskuler dengan interval 10 hari setelah penyuntikan pertama. Kambing-kambing yang memperlihatkan gejala berahi dikawinkan secara inseminasi buatan. Diagnosis kebuntingandilakukan dengan menggunakan USG 30 hari setelah inseminasi. Data onset berahi dianalisis menggunakan analisis varian, sedangkan persentase berahi dan kebuntingan dianalisis secara deskriptif. Onset berahi ketiga kelompokperlakuan masing-masing adalah 40,80,57 36,00,57 dan 50,41,52 jam (P0,05). Persentase berahi pada ketiga kelompok adalah sama yakni 100%, sedangkan persentase kebuntingan pada kelompok I, II, dan III masing-masing adalah 100, 80, dan 60%. Dari hasil penelitian dapat disimpulkan bahwa onset dan persentase berahi tidak dipengaruhi oleh ketiga prostaglandin komersial yang berbeda tetapi berpengaruh terhadap persentase kebuntingan kambing PE.

Co-Authors Ademi, Gressha Vionalle Ahmad Syakir Arfida Boedirochminarni Arsih, Ulfi Widi Asmaul Husna Azhari Azhari Darmawi . Darmawi Darmawi Darmawi Darmawi Erdiansyah Rahmi Erdiansyah Rahmi Gressha Vionalle Ademi Hafizuddin Hafizuddin Hamdan Hamdan Hamdan Hamdan Haryadi Haryadi, Hayadi Irfannur, Irfannur Jumadi Muliari Muliari Mustafa Kamal Mustaqim Mustaqim Mustaqim Mustaqim, Mustaqim Mutasar NURLIANA NURLIANA Rini Mastuti Rinidar Rinidar Riza Rahman Hakim Safika S, Safika Siti Hajar Syakir, Ahmad Tongku Nizwan Siregar Ulfi Widi Arsih Wahyuni, Mida Yandi Syahputra Yus Mochamad Cholily Yusrizal Akmal

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