Article Per Year (5 Year)
p-Index From 2020 - 2025
0.23
P-Index
This Author published in this journals
All Journal Jurnal Biologi Udayana JURNAL TEKNOLOGI LINGKUNGAN BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Syntax Literate: Jurnal Ilmiah Indonesia ANNALES BOGORIENSES Teknologi Indonesia Annales Bogorienses
NUNIK SULISTINAH
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Education Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Social Sciences Other

Published : 34 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : Teknologi Indonesia

 1 
BIOTRANSFORMASI 3-SIANOPIRIDIN MENJADI NIKOTINAMIDA DENGAN SEL CORYNEBACTERIUM D5 SEBAGAI BIOKATALIS Sunarko, Bambang; Sulistinah, Nunik
Teknologi Indonesia Vol 34 (2011)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (16.048 KB) | DOI: 10.14203/jti.v34iKhusus.46

Abstract

A number of microbes isolated from industrial effl uents were studied to characterise their capability to convert 3-cyanopiridine. Isolate D5, identifi ed as Corynebacterium D5, was able to hydrolise 3-cyanopridin to nicotinamide, nicotinic acid, and ammonia catalyzed by nitrile-hydratase and amidase. The activity of nitrile-hydratase and amidase of Corynebacterium D5 were 0.364 U nad 0.200 U, respectively. The optimum hydrolisis of 3-cyanopiridine were occurred at 25oC and pH 7.0. Corynebacterium D5 exhibited the best growth (0.43 g cells dry weight/liter), when 0.5% (v/v) acetonitrile and 20 mM 3-cyanopiridine were used as sources of carbon, energy, and nitrogen.
RHODOCOCCUS PYRIDINIVORANS STRAIN TPIK, A NITRILE-DEGRADING BACTERIUM ISOLATED FROM TAILING POND OF ANTAM GOLD MINING INDUSTRY , PONGKOR Sulistinah, Nunik; Riffiani, Rini; Sunarko, Bambang
Teknologi Indonesia Vol 38, No 2 (2015)
Publisher : LIPI Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jti.v38i2.158

Abstract

Nitriles are widely manufactured and extensively used by chemical industries as synthesis intermediates. Although these compounds are generally highly toxic, due to their cyano functional group, they can be used by some microorganisms as carbon and/or nitrogen sources. Nitrilase catalyzes the direct cleavage of nitriles to the corresponding acids and ammonia, where as nitrile-hydratase catalyzes the hydratation of nitriles to amides. Applications of these nitrile-converting enzymes are now increasingly recognized for the production of several pharmaceutically important compounds and fine chemicals. By virtue of their capability to eliminate highly toxic nitriles, the enzymes also play a significant role in protecting the environment. Accordingly, it is very important to find microorganisms that have a great capacity to utilize or degrade nitriles. Recently, a bacterial isolate TPIK which shows high nitrile degrading activity has been isolated. The strain was isolated from tailing pond of gold mine activity by selective enrichment methods. TPIK was shown to be capable of degrading high concentrations of nitrile (up to approx. 1 M acetonitrile). Colonies of TPIK are light orange in colour and have irregularly round wrinkles. The cells of TPIK are non-spore-forming, non motile and Gram-positive, but are Gram-variable in old cultures. The cells are rods and brached fillaments during the early growth phase and then fragmented into short rods and cocci. The taxonomic position of TPIK was clarified using molecular genetic methods. The phylogenetic tree showed that bacterial isolate TPIK falls within an evolutionary radiation comprising Rhodococcus species and is most closely related to the type strain of Rhodococcus pyridinivorans, sharing 99% 16S rDNA similarity. To the best of our knowledge, this was the first time that Rhodococcus pyridinivorans has been described as containing nitrile-degrading enzymes. Usually, this bacterial strain has been studied for its ability to degrade carcinogenic compounds, like pyridine.
Co-Authors Adityarini Adityarini Adityarini, Adityarini Afianisa, Salma Ahmad Thontowi Antonius, S Bambang Sunarko BAMBANG SUNARKO Endang Saepudin, Endang Kaban, Joseva Sudiati Kaban, Joseva Sudiati Maman Rahmansyah Meyer, Ortwin Meyer, Ortwin Munandar, Hendra Nieder, Maria Nieder, Maria Rini Riffiani Soenarko, Bambang Tambunan, Usman Sumo F Usman Sumo F Tambunan