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All Journal International Journal of Public Health Science (IJPHS) ACTA VETERINARIA INDONESIANA Saintika Medika: Jurnal Ilmu Kesehatan dan Kedokteran Keluarga. Jurnal Sain Veteriner SOCA: Jurnal Sosial Ekonomi Pertanian Buletin Veteriner Udayana Jurnal AGROTEKNOLOGI Jurnal Ilmu Dasar Jurnal Natur Indonesia Jurnal Kedokteran Syiah Kuala Media Penelitian dan Pengembangan Kesehatan e-Journal Pustaka Kesehatan Jurnal Kedokteran dan Kesehatan : Publikasi Ilmiah Fakultas Kedokteran Universitas Sriwijaya Indonesian Journal of Urology TEMA (Jurnal Tera Ilmu Akuntansi) JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Journal of Agromedicine and Medical Sciences Jurnal Bioteknologi & Biosains Indonesia (JBBI) Medical Laboratory Technology Journal Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan NurseLine Journal SRIWIJAYA JOURNAL OF MEDICINE Journal of Health Sciences JHECDs: Journal of Health Epidemiology and Communicable Diseases Journal of the Indonesian Medical Association : Majalah Kedokteran Indonesia Berita Kedokteran Masyarakat Jurnal Ners dan Kebidanan (Journal of Ners and Midwifery)
ENNY SUSWATI
Department Of Microbiology, Faculty Of Medicine, University Of Jember, Jember, 68121 Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Library & Information Science Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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Journal : Jurnal Bioteknologi

 1 
PERAN PROTEIN PILI 11 kDa Streptococcus pneumoniae SEBAGAI PROTEIN HEMAGLUTININ DAN ADHESIN Mufida, Diana Chusna; Salsabila, Yuna Annisa; Suswati, Enny; Hermansyah, Bagus; Agustina, Dini
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v7i1.3930

Abstract

Role of Pili Protein 11 kDa of Streptococcus pneumoniae as Hemagglutinin and Adhesin Protein Streptococcus pneumoniae has pili which play roles in adhesion, colonization of nasopharyngeal epithelial cells, and phagocytic inhibition of immune cells. This study aimed to determine the characteristics of the 11 kDa pili protein as hemagglutinin and adhesin, as well as their immune responses. The 11 kDa pili protein from S. pneumoniae was isolated by SDS-PAGE, purified by electroelution and dialysis. Hemagglutination and adhesion tests were carried out on the protein, and western blotting of the polyclonal antibody immune responses were evaluated. Hemagglutination test showed that the 11 kDa pili protein played a role in the hemagglutination process up to 2-time dilution. Adhesion test showed there was a correlation between the dose of the protein and the bacteria attached to the epithelial cells. The Pearson correlation test showed a P value of 0.010 and a correlation coefficient of R = -90.919. Quadratic regression test produced R2 = 0.974. Western blotting test showed that 11 kDa pili protein polyclonal antibodies recognized 67 kDa and 11 kDa pili proteins. The study concluded that the 11 kDa S. pneumoniae pili protein acted as hemagglutinin and adhesin, and the polyclonal antibody protein responded to 67 pDa and 11 kDa BM pili proteins.Keywords: adhesin, hemagglutinin, pili, protein 11 kDa, Streptococcus pneumoniae ABSTRAKStreptococcus pneumoniae memiliki pili yang berperan dalam adhesi, kolonisasi sel epitel nasofaring, serta sebagai inhibitor fagositosis sel imun. Penelitian ini bertujuan mengetahui karakteristik protein pili 11 kDa sebagai hemagglutinin dan adhesin serta respons imunnya. Protein pili 11 kDa dari bakteri S. pneumoniae diisolasi secara SDS-PAGE, dipurifikasi dengan elektroelusi dan dialysis. Uji hemaglutinasi dan adhesi dilakukan pada protein tersebut, serta dievaluasi respon imun poliklonal antibodinya secara western blotting. Uji hemaglutinasi menunjukkan protein pili 11 kDa berperan dalam proses hemaglutinasi hingga pengenceran 2 kali. Uji adhesi menunjukkan korelasi antara dosis protein dan bakteri yang menempel pada sel epitel. Uji korelasi Pearson menunjukkan P value 0,010 dan koefisien korelasi R = -0,919. Uji regresi Quadratic menghasilkan R2 = 0,974. Uji Western blotting menunjukkan antibodi poliklonal protein pili 11 kDa mengenali protein pili 67 kDa dan 11 kDa. Penelitian ini berkesimpulan protein pili 11 kDa S. pneumoniae berperan sebagai hemaglutinin dan adhesin, serta antibodi poliklonal protein tersebut memberi respons terhadap protein pili BM 67 kDa dan 11 kDa. 
PERAN PROTEIN PILI 11 kDa Streptococcus pneumoniae SEBAGAI PROTEIN HEMAGLUTININ DAN ADHESIN Diana Chusna Mufida; Yuna Annisa Salsabila; Enny Suswati; Bagus Hermansyah; Dini Agustina
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 7 No. 1 (2020): June 2020
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29122/jbbi.v7i1.3930

Abstract

Role of Pili Protein 11 kDa of Streptococcus pneumoniae as Hemagglutinin and Adhesin Protein Streptococcus pneumoniae has pili which play roles in adhesion, colonization of nasopharyngeal epithelial cells, and phagocytic inhibition of immune cells. This study aimed to determine the characteristics of the 11 kDa pili protein as hemagglutinin and adhesin, as well as their immune responses. The 11 kDa pili protein from S. pneumoniae was isolated by SDS-PAGE, purified by electroelution and dialysis. Hemagglutination and adhesion tests were carried out on the protein, and western blotting of the polyclonal antibody immune responses were evaluated. Hemagglutination test showed that the 11 kDa pili protein played a role in the hemagglutination process up to 2-time dilution. Adhesion test showed there was a correlation between the dose of the protein and the bacteria attached to the epithelial cells. The Pearson correlation test showed a P value of 0.010 and a correlation coefficient of R = -90.919. Quadratic regression test produced R2 = 0.974. Western blotting test showed that 11 kDa pili protein polyclonal antibodies recognized 67 kDa and 11 kDa pili proteins. The study concluded that the 11 kDa S. pneumoniae pili protein acted as hemagglutinin and adhesin, and the polyclonal antibody protein responded to 67 pDa and 11 kDa BM pili proteins.Keywords: adhesin, hemagglutinin, pili, protein 11 kDa, Streptococcus pneumoniae ABSTRAKStreptococcus pneumoniae memiliki pili yang berperan dalam adhesi, kolonisasi sel epitel nasofaring, serta sebagai inhibitor fagositosis sel imun. Penelitian ini bertujuan mengetahui karakteristik protein pili 11 kDa sebagai hemagglutinin dan adhesin serta respons imunnya. Protein pili 11 kDa dari bakteri S. pneumoniae diisolasi secara SDS-PAGE, dipurifikasi dengan elektroelusi dan dialysis. Uji hemaglutinasi dan adhesi dilakukan pada protein tersebut, serta dievaluasi respon imun poliklonal antibodinya secara western blotting. Uji hemaglutinasi menunjukkan protein pili 11 kDa berperan dalam proses hemaglutinasi hingga pengenceran 2 kali. Uji adhesi menunjukkan korelasi antara dosis protein dan bakteri yang menempel pada sel epitel. Uji korelasi Pearson menunjukkan P value 0,010 dan koefisien korelasi R = -0,919. Uji regresi Quadratic menghasilkan R2 = 0,974. Uji Western blotting menunjukkan antibodi poliklonal protein pili 11 kDa mengenali protein pili 67 kDa dan 11 kDa. Penelitian ini berkesimpulan protein pili 11 kDa S. pneumoniae berperan sebagai hemaglutinin dan adhesin, serta antibodi poliklonal protein tersebut memberi respons terhadap protein pili BM 67 kDa dan 11 kDa. 
Co-Authors Abu Khoiri Aditya Oktavianto Ahmad Hasbi Al-Muzaky Ahmad Hasbi Al-Muzaky Alfianti, Nita Angga Mardro Raharjo Anggraini, Nanda Dewi Anisa Rizca Putri APRI KUNTARININGSIH Arifah Nur Hasanah Ariska Nur Aida Aryasatya, Dimas Widyadhana Bhanu asihanti rosita ferdiana Azham Purwandhono Bagus Hermansyah Bagus Hermansyah Bagus Satrio Pambudi Bagus Satrio Pambudi, Bagus Satrio Cholis Abrori, Cholis Desie D. Wisudanti Desie Dwi Wisudanti Devi Chintya Kumalasari Dian H. Purnamasari Diana C Diana C. Mufida Diana Chusna Mufida Dini Agustina Dwita Aryadina Dwita Aryadina Rachmawati Elly Nurus Sakinah, Elly Nurus Elvia Rahmi Marga Putri Erfan Efendi Habib Mustofa Haq, Hanifah S Ika Rahmawati Sutejo Indra Samudra Rahmat Irawan Fajar Kusuma Jauhani, Muhammad Afiful Jauhar Firdaus Joko Mariyono Kirana Nadyatara Kristianningrum Dian Sofiana Lailatis Shofia Laili, Nikmatul Laksmi Indreswari Lutfadaturroifa, Alya Wafaul M. Ali Shodikin Misnawi Misnawi Misnawi Misnawi Mufida Mufida Mufida, Diana C Muhammad A. Shodikin Muhammad Alif Taryafi Mukarromah, Laylatul Fitriyah Nadhiva, Alvien Zahrotun Nexia Nevarachell Onny Amirsyah Nisrina Salsabila Firmansyah Nita Alfianti Nugraha, Bima Setia Sandya NUR K. AGUSTIN Nurhayati Nurhayati Pratama, Dava Rizky Prayoga Adinawer Sirait Probosutiksna, Nuno Febrian Probosutiksna, Nuno Febrian Putra Uta, Diego Rafi Putri, Elvia Rahmi Marga Putu Ayu Laksmi Lestari Raharjo, Angga M Rahmat, Indra Samudra Regina Finka Risty Pradana Linggan Wangi Rosdiana, Fetri Rumastika, Nindya Shinta S.Pd. M Kes I Ketut Sudiana . Sakinah, Elly N Salsabila, Yuna Annisa Saskia Mediawati septa surya wahyudi, septa surya Shabri, Hammam Arif Shodikin, M Ali Shodikin, M. Ali Shofia, Lailatis Sudarmanto, Yohanes Supangat Supangat Supangat Supangat Supangat Suryono Suryono Syahputri, Siti Ananda Hardita Ulfa Elfiah Wahyuning Ati Ashari Wiwien Sugih Utami Wiwin Sugih Utami Yudha Nurdian Yudha Nurdian Yuna Annisa Salsabila Yunita Armiyanti Yunita Wulansari