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All Journal Nurse Media Journal of Nursing Bioma : Berkala Ilmiah Biologi Jurnal Penelitian Pendidikan Guru Sekolah Dasar Acta Interna : The Journal of Internal Medicine Jurnal Edukasi Universitas Jember Jurnal S2 Pendidikan Sains MAGISTRA Pharmacon Religious: Jurnal Studi Agama-Agama dan Lintas Budaya Jurnal Penelitian Pembelajaran Fisika Jurnal Pendidikan Biologi Health Notions Ekspektra: Jurnal Bisnis & Manajemen BIOPROSPEK Jurnal Pendidikan Kebutuhan Khusus Cakrawala Dini : Jurnal Pendidikan Anak Usia Dini Equilibrium: Jurnal Ekonomi-Manajemen-Akuntansi Eksis: Jurnal Riset Ekonomi dan Bisnis Jurnal EDUCATIO: Jurnal Pendidikan Indonesia Jurnal Pendidikan Edutama TEMALI : Jurnal Pembangunan Sosial Jurnal 'Aisyiyah Medika Jurnal Ilmiah Kesehatan JURNAL ILMIAH OBSGIN : Jurnal Ilmiah Ilmu Kebidanan & Kandungan NURSING UPDATE JURNAL ILMIAH ILMU KEPERAWATAN Khazanah Sosial Jurnal Untuk Masyarakat Sehat (JUKMAS) Journal of Digital Education, Communication, and Arts (DECA) Jurnal Mediasosian : Jurnal Ilmu Sosial dan Administrasi Negara Hanifiya: Jurnal Studi Agama-Agama JPEKBM (Jurnal Pendidikan Ekonomi, Kewirausahaan, Bisnis dan Manajemen) Community Development Journal: Jurnal Pengabdian Masyarakat Jurnal Pengabdian Masyarakat Khatulistiwa Prosiding Conference on Research and Community Services Epidemiology and Society Health Review (ESHR) Bioscientist : Jurnal Ilmiah Biologi Jurnal Riset dan Inovasi Pembelajaran Business and Accounting Education Journal Abrahamic Religions: Jurnal Studi Agama-Agama Kabanti : Jurnal Kerabat Antropologi Formosa Journal of Sustainable Research (FJSR) Jurnal Ilmiah Pendidikan Dasar (JIPDAS) Journal of Management, Accounting, General Finance and International Economic Issues (MARGINAL) Journal of Community Empowerment and Innovation Journal of Comprehensive Science Juru Rawat. Jurnal Update Keperawatan Jurnal Fuaduna: Jurnal Kajian Keagamaan dan Kemasyarakatan Millah: Journal of Religious Studies Sewagati Neraca: Jurnal Ekonomi, Manajemen dan Akuntansi Java Nursing Journal Jurnal Pengembangan Ilmu dan Praktik Kesehatan Journal of Digital Business and Innovation Management Jurnal Pharma Bhakta JAPP : Jurnal Akuntansi, Perpajakan dan Portofolio Journal Of Bionursing Southeast Asian Language and Literature Studies Kajian Ilmiah Mahasiswa Administrasi Publik (KIMAP) Jurnal Pengabdian Keperawatan Dan Kesehatan Holistik (JPKKH) Indonesian Journal of Aerospace Jurnal Pengabdian Kepada Masyarakat (JP-MAS)
Dwi Wahyuni
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Journal : Bioscientist : Jurnal Ilmiah Biologi

 1 
Potensi Infusa Daun Paitan (Tithonia diversifolia) sebagai Larvasida Terhadap Mortalitas dan Morfologi Larva Aedes aegypti Wahyuni, Dwi; Swandono, Hari Untarto; Purwani, Asih Imulda Hadi; Pramasari, Nadia; Putri, Sherlyna Rizkiya
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12902

Abstract

Dengue Hemorrhagic Fever is an infectious disease caused by the Dengue virus through the Aedes aegypti mosquito vector, which is controlled both at the larval and adult stages. The use of chemicals that are difficult to degrade in nature can pollute the environment. The Paitan plant (Tithonia diversifolia) contains secondary metabolite compounds that have the potential to be natural larvicides. This study aims to determine the natural larvicidal activity of Paitan leaf infusion. This study is an experimental study. The results of this study indicate that (1) Paitan leaf infusion contains alkaloid, flavonoid, terpenoid, saponin, phenolic, and tannin compounds. At concentrations of 1.8%, 3.2% and 5.6%, the mortality percentage results were 41.30%, 50.60% and 60% respectively; (2) The administration of Paitan leaf infusion has an effect on morphological damage Aedes aegypti larvae in the thorax, digestive tract and siphon. The content of different metabolite compounds causes different levels of morphological damage at each concentration
Korelasi Kadar Total Flavonoid Ekstrak dan Fraksi Polar Kelopak Rosella (Hibiscus sabdariffa L.) Terhadap Aktivitas Antioksidan dengan Metode DPPH (2,2-Difenil-1-Pikrilhidrazil) Sari, Fita; Hidayatul, Fathul; Sariwati, Atmira; Wahyuni, Dwi; Hesturini, Rosa Juwita
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13205

Abstract

Senyawa metabolit sekunder rosella memiliki manfaat kesehatan, seperti hipertensi, diabetes, dan penyakit kardiovaskular. Rosella digunakan sebagai bahan minuman kesehatan, karena senyawa aktifnya seperti antosianin, polisakarida, dan flavonoid. Flavonoid dalam rosella diduga memiliki aktivitas antioksidan dalam menangkal radikal bebas penyebab penyakit. Penelitian ini bertujuan melihat profil fitokimia ekstrak dan fraksi polar kelopak rosella dengan Kromatografi Lapis Tipis (KLT) dan dilanjutkan menguji kadar total flavonoid serta aktivitas antioksidan dengan DPPH (1,1-difenil-2-pikrilhidrazil). Studi ini merupakan penelitian kualitatif dan kuantitatif, untuk mendeteksi profil fitokimia dari ekstrak dan fraksi kelopak rosella dilanjutkan uji kadar total flavonoid dan aktivitas antioksidan. Hasil penelitian menunjukkan bahwa (1) adanya senyawa flavonoid, ditandai bercak warna biru pada plat KLT, (2) uji kadar total flavonoid dari ekstrak menunjukkan sebesar 139,4321 ppm sedangkan fraksi polar 204,8334 ppm, (3) uji aktivitas antioksidan menunjukkan bahwa ekstrak rosella memiliki aktivitas lemah dengan nilai IC50 sebesar 656,4775 ppm, sedangkan fraksi lebih kuat dengan nilai IC50 sebesar 110,1132 ppm. Dengan demikian dapat disimpulkan bahwa ekstrak etanol dan fraksi polar kelopak rosella diduga memiliki senyawa flavonoid yang berperan sebagai antioksidan namun fraksi lebih kuat dibandingkan dengan ekstrak etanol kelopak rosella.
Karakterisasi dan Potensi Ekstrak Daun Paitan (Tithonia diversifolia) sebagai Penolak Nyamuk Aedes aegypti Wahyuni, Dwi; Swandono, Hari Untarto; Mawardika, Herlinda; Prana, Mega Yuliya
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.8844

Abstract

The paitan plant (Tithonia diversifolia) contains alkaloids, saponins, flavonoids, tannins and phenolics which have the potential to prevent Dengue Hemorrhagic Fever (DHF). This plant also has various insecticidal activities. In society, there are many mentions of the paitan plant, so people need to know the characteristics of this paitan plant. The aim of this research is to determine the morphological characterization and specific fragments of the paitan plant, as well as to determine the activity and effective concentration of the ethanol extract of paitan leaves as a repellent for the Aedes aegypti mosquito. Two types of methods were used, namely descriptive qualitative for observing morphology, specific fragments, pollen, and phytochemical screening, and experimental methods for mosquito repellent testing, and calculating the protective power of the ethanol extract of paitan leaves. The results obtained are flowers consisting of two shapes, namely ribbon flowers in the outer circle, infertile, and tube shapes in the inner circle. Stomata are anomocytic type, while trichomes are non-glandular multicellular type. Pollen characteristics, namely small size, colpate, number 3, aperture shaped trizonocolpate with echinate ornamentation. The screening results for the ethanol extract of paitan leaves were positive for containing metabolite compounds, such as flavonoids, alkaloids, tannins, saponins, terpenoids and phenolics. The results of the mosquito repellent test showed that the higher the concentration of the extract, the fewer mosquitoes that landed. Calculation of protective power shows that the most effective concentration in repelling mosquitoes is 15%, namely 93.64%. The conclusion is that paitan (Tithonia diversifolia) leaf extract has potential activity as a repellent against Aedes aegypti mosquitoes with the most effective concentration being 15%.
Karakterisasi dan Uji Aktivitas Antimikroba Bakteri Kandidat Probiotik dari Terasi Udang Rebon Mawardika, Herlinda; Pertiwi, Krisna Kharisma; Wahyuni, Dwi; Aulia, Qosania Wilda
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.8981

Abstract

Probiotics are live microorganisms that are widely used to help maintain a healthy digestive system. Some compounds secreted by these bacteria have a bactericidal effect on pathogenic and putrefactive bacteria. Various types of probiotic bacteria can be obtained from fermented foods and drinks. This research aims to characterize bacteria from rebon shrimp paste in Macajah Village, Tanjung Bumi District, Bangkalan Regency, East Java Province which have potential as probiotics and test their antimicrobial activity against pathogenic bacteria. Bacteria were isolated using the Pour Plate technique using de Man Rogosa Sharpe Agar media. The bacterial isolates were then characterized by observing colony morphology, cells and biochemical properties. Next, antibacterial activity was tested using the well diffusion method. The results showed that the four bacteria obtained from the samples were gram positive, round and rod shaped, catalase negative, homofermentative, grew at a temperature of 37-45°C, survived at NaCl concentrations of 4 and 6.5%, low pH, and bile salts. According to the characterization results, three bacterial isolates belonged to the genus Enterococcus and one other isolate belonged to the genus Bacillus. Bacillus isolate is a potential probiotic candidate, because it has inhibitory activity against Escherichia coli and Staphylococcus aureus.
Penetapan Kadar Kuersetin pada Obat Herbal yang Mengandung Ekstrak Tempuyung (Sonchus arvensis) Menggunakan KLT Densitometri Purwani, Asih Imulda Hari; Pertiwi, Krisna Kharisma; Wahyuni, Dwi; Nurhayati, Rachma
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.11260

Abstract

Quercetin are the main marker compounds in extracts of Sonchus arvensis which show nephrolithiasis activity. For quality control of herbal medicines, Densitometric Thin Layer Chromatography (TLC) method has been developed in this study using quercetin as phytochemical markers. Objective: Determination for quercetin in herbal medicines. Determination of quercetin by determining the composition of the mobile phase, the wavelength of the analysis, the saturation time, the elution distance and the volume of the spot. The parameters observed in the optimization method and stability test are area, retardation factor (Rf), peak shape and resolution (Rs). The composition of selected mobile phase which results good separation and symmetrical peak shape is chloroform: methanol: dichloromethane: acetonitrile: formic acid (6: 2: 2: 0.05: 0.05 v/v/v/v/v) with a wavelength of 335 nm, time of saturation is 30 minutes, elution distance is 8 cm, volume of spots is 10 µL and Rf 0.48. In this study, it was shown that there were no factors that had a significant effect so that the method could be applied for the determination of quercetin levels in herbal medicines.The research data showed that the range of levels on quercetin 3.26%.
Potensi Infusa Daun Paitan (Tithonia diversifolia) sebagai Larvasida Terhadap Mortalitas dan Morfologi Larva Aedes aegypti Wahyuni, Dwi; Swandono, Hari Untarto; Purwani, Asih Imulda Hadi; Pramasari, Nadia; Putri, Sherlyna Rizkiya
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12902

Abstract

Dengue Hemorrhagic Fever is an infectious disease caused by the Dengue virus through the Aedes aegypti mosquito vector, which is controlled both at the larval and adult stages. The use of chemicals that are difficult to degrade in nature can pollute the environment. The Paitan plant (Tithonia diversifolia) contains secondary metabolite compounds that have the potential to be natural larvicides. This study aims to determine the natural larvicidal activity of Paitan leaf infusion. This study is an experimental study. The results of this study indicate that (1) Paitan leaf infusion contains alkaloid, flavonoid, terpenoid, saponin, phenolic, and tannin compounds. At concentrations of 1.8%, 3.2% and 5.6%, the mortality percentage results were 41.30%, 50.60% and 60% respectively; (2) The administration of Paitan leaf infusion has an effect on morphological damage Aedes aegypti larvae in the thorax, digestive tract and siphon. The content of different metabolite compounds causes different levels of morphological damage at each concentration
Korelasi Kadar Total Flavonoid Ekstrak dan Fraksi Polar Kelopak Rosella (Hibiscus sabdariffa L.) Terhadap Aktivitas Antioksidan dengan Metode DPPH (2,2-Difenil-1-Pikrilhidrazil) Sari, Fita; Hidayatul, Fathul; Sariwati, Atmira; Wahyuni, Dwi; Hesturini, Rosa Juwita
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13205

Abstract

Senyawa metabolit sekunder rosella memiliki manfaat kesehatan, seperti hipertensi, diabetes, dan penyakit kardiovaskular. Rosella digunakan sebagai bahan minuman kesehatan, karena senyawa aktifnya seperti antosianin, polisakarida, dan flavonoid. Flavonoid dalam rosella diduga memiliki aktivitas antioksidan dalam menangkal radikal bebas penyebab penyakit. Penelitian ini bertujuan melihat profil fitokimia ekstrak dan fraksi polar kelopak rosella dengan Kromatografi Lapis Tipis (KLT) dan dilanjutkan menguji kadar total flavonoid serta aktivitas antioksidan dengan DPPH (1,1-difenil-2-pikrilhidrazil). Studi ini merupakan penelitian kualitatif dan kuantitatif, untuk mendeteksi profil fitokimia dari ekstrak dan fraksi kelopak rosella dilanjutkan uji kadar total flavonoid dan aktivitas antioksidan. Hasil penelitian menunjukkan bahwa (1) adanya senyawa flavonoid, ditandai bercak warna biru pada plat KLT, (2) uji kadar total flavonoid dari ekstrak menunjukkan sebesar 139,4321 ppm sedangkan fraksi polar 204,8334 ppm, (3) uji aktivitas antioksidan menunjukkan bahwa ekstrak rosella memiliki aktivitas lemah dengan nilai IC50 sebesar 656,4775 ppm, sedangkan fraksi lebih kuat dengan nilai IC50 sebesar 110,1132 ppm. Dengan demikian dapat disimpulkan bahwa ekstrak etanol dan fraksi polar kelopak rosella diduga memiliki senyawa flavonoid yang berperan sebagai antioksidan namun fraksi lebih kuat dibandingkan dengan ekstrak etanol kelopak rosella.
Co-Authors Abdul Kadir Ady Soesetijo, Ady Akbar, Muhammad Farhan Almarsaban, Almarsaban Alviyanti, Dina Ama, Petrus Geroda Beda Andini, Mariska Anggraeni, Apprillia Anggraeni, Nyi Aida Anitasari, Dhesy Anjani, Mardhiana Dwi Ardiyansyah, Ridho Arini Dwi Larasatining Retnowati, Arini Dwi Larasatining Armaini, Armaini Armawadin, Irsyad Daffa Asih Imulda Hari Purwani Asniah Asniah Asrulla, Asrulla Aulia, Qosania Wilda Ayu Ningsih, Silvi Putri Beda Ama, Petrus Geroda Burhan, Asmat Busro, B. Depita Ariningtyas Dera Dwi Herawati Deshinta Putri Mulya Desi Rusmiati, Desi Devi Novitasari, Devi Devita, Risa Dewi, Sri Rukmini Dian Wahyuning Tyas Dinaloni, Diah Efriyanisari, Efriyanisari Eka Agustina, Eka Erwin Novita Sari Farianingsih, Farianingsih Fauziah, Dafa Feriatul Qiptiyah, Feriatul Fierda Nurany, Fierda Fihrin Fihrin Fita Sari, Fita Fitrah Dewi Mustikasari Giyatmi Giyatmi Hafrizdha Chandra Y Hari Untarto Swandono Harini Hartono, Arif Herlinda Mawardika HERU SUBRATA Hidayatul, Fathul Husna, Wal’idatul Ida Kristianingsih Iffah, Jauhara Dian Nurul Iis Nur Asyiah Ina Darliana Jarwanti, Jarwanti Jeka, Firdaus Jekti Prihatin Jubaidah, Asih Kamila, Fanisa Ayu Cahya Karista, Karen Karlina, Intan Kastori F , Aris Khairul Ikhwan Khairunnisa Batubara Khasanah, Siti Ma’rifatul Khomisah komalasari , Sartika KRISNA KHARISMA PERTIWI KURNIAWATI, YUYUN La Ode Aris Laksanano, Gayuh Siska Laurenza, Dinar Legawati , Sri Legawati Lestari, Julita Lucia Endang Hartati, Lucia Endang Lukman Hakim Maemunah , Azis Maemunah, Azis Maisah Maisah Mardiatul Hasanah Martalia, Martalia Masruroh, Ani Mochammad Iqbal Muhammad Yusuf Muslimin Muslimin Nadia Pramasari Ni'mah, Ni'mah Norhayati, Dewi Nur Aini NURUL HIDAYAH Nurwiani, Nurwiani Octavia, Indah Andika Pane , Efi Irwansyah Pham, Huong Thi Thu Prana, Mega Yuliya Prasetijwowati, Tri Pratama, Fadhiil Novriadi Priastika, Awaluci Puspitaningsari, Mecca Putri, Amirah Nuraini Lianti Putri, Sherlyna Rizkiya Rachma Nurhayati Rahayu, Martini Rahman, Muhammad Fajar Wahyudi Rahmawati, Miranda Wulandari Rambe, Riris Nurkholidah Ratna, Dewi Retnowulan, Heni Rezeki , R. Sri Ridlo, Zainur Rasyid Riska Dhenabayu Rismawati , Rismawati Riyanti, Neni Rizka Elan Fadilah, Rizka Elan Rohmatin, Homsiatur Rosa Juwita Hesturini Rosa Susanti Rosa, Lidiya Praktika Rosadi, Kemas Imron Rozana, Kennis S Suratno Sabna, Anjali Saksitha, Deassy Arestya Saloma Samadi W Bakar, Samadi W Sara Munthe, Atika Sari PDW, Kumala Sariwati, Atmira Sefriyono Sefriyono Sekarrini, Loveria Selly Rizki Yanita Sembiring, Evaliata Br. Sihombing, Nurma Sari Siti Murdiyah Slamet Hariyadi Sri Katoningsih Sri Wilujeng Srimulyaningsih, Reni Suciati Sudarisman Sudiastuti, Sudiastuti Sudrajat Sudrajat Sugeng Sutiarso Sugiyarto Sugiyarto Sukur, La Ode Sulistyaningsih Sulistyaningsih Sulthan Ahmad Suryani Harahap, Efi Susanti, Dhina Ayu Susilowati, Lina Sutarto Sutarto Suwanda, Azlan Swandono, Hari Tan, Ishak Tantut Susanto Tiarnida Nababan Tomi Utomo tri wahyuni Trio Ageng Prayitno, Trio Ageng Wachju Subchan Wenang Anurogo, Wenang Widjayanti, Trisna Budy Widya Resti Susanti Widyarani, Destia Wilis Sukmaningtyas Yulazri, Yulazri Yuliani Yuliani