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Yustini Alioes
Fakultas Kedokteran Universitas Andalas
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Journal : Bioma : Jurnal Ilmiah Biologi

 1 
ISOLATION OF AnsB GENE FRAGMENT ENCODING L-ASPARAGINASE 2 ENZYME FROM Serratia plymuthica UBCF_13 AND IT’S IN-SILICO DOMAIN CHARACTERISTIC Ananda, Abi Awfa Rahman; Nursyafi, Fauzan Syarif; Aliya, Lisana Shidiqqin; Hasibuan, Imron Martua; Alioes, Yustini; Endrinaldi , Endrinaldi; Adrial, Adrial; Elmatris , Elmatris; Jamsari, Jamsari; Lily Syukriani
BIOMA : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2025): April 2025
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/n5mfhy69

Abstract

Acute lymphoblastic leukemia(ALL) is one of the cancer diseases often occurs in children and causes high mortality in children. One of the chemotherapy treatment suggested is using L-Asparaginase 2. However due to its difficult production process making this approach expensive for the public. Therefore production technology of this enzyme is crucial enabling cheaper for ALL treatment. This study aimed to isolate the AnsB gene sequence from Serratia plymuthica UBCF_13 and perform its further in-silico analysis. The research was started by designing specific primers for the AnsB gene, isolating the AnsB gene fragment using PCR-based approach, sequencing the AnsB gene fragment, cloning the fragment to the plasmid vector and further transformed into E. coli DH5α cell. Further data analysis was carried out using some bioinformatics tools such as BLAST, MEGA X, I-TASSER InterPro. Sequence data result successfully verified that the full length of AnsB gene is 1047 bp. InterPro analysis indicated that the L-Asparaginase 2 from S. plymuthica UBCF_13 has 2 domains, namely L-Asparaginase N-terminal spanning from amino acid 26 to 216, while its C-terminal spanned from amino acid 235 to 345. The physical fragment of the gene was also successfully cloned to the pGEM-T Easy vector and subsequently transformed into E. coli DH5α cell. This result provided information for alternative sources of L-Asparaginase 2 and it’s possible engineering.
Co-Authors Adrial Adrial, Adrial Aida Fitriana Aliya, Lisana Shidiqqin Almurdi Almurdi amalia Kartika Amelia Amelia Amirah Zatil Izzah Ananda, Abi Awfa Rahman Andree, Azzahra Anggita Anggraini, Syahzalya Anggrainy, Fenty Annisa Pratiwi Asterina Asterina Asterina, Asterina Ayu Ratna Sari Azzati, Normaida Baizura Benni Raymond Catur Hadi Arief Dermawan Chindy Jhonel Putri Deddy Saputra Desmawati Desmawati Desmawati Desmawati Dessy Arisanty Desy Arisanti Desy Arisanti Dia Rofinda, Zelly Dia Dian Pertiwi Dinda Aprilia Dwi Yulia Efendi, Altio Efendi, Altio Efrida Efrida Elmatris , Elmatris Elmatris Sy Elmatris Syamsir Elmatris, Elmatris Elsi Wineri Endrinaldi Endrinaldi , Endrinaldi Eni Rahmi Eti Yerizel Eva Chundrayetti Eva Decroli F, Annisa Maida Fadil Oenzil Fadrian, Fadrian Faradila Faradila Fika Tri Anggraini Harsa Rusda Hasibuan, Imron Martua Hasnah Hasnah Hasnah, Hidayatul Hasnah, Widya Jummatul Hayyumahdania Reswan Hendra Permana Hirowati Ali Husnil Kadri Husnil Wardiyah Indah Febranambela Jovie Irwan Jamsari Jamsari Kartika, amalia Lili Irawati Lily Syukriani Lisma Yendri Lydia Susanti, Lydia M Ikhsan Mahata, Liganda Endo Mentari Artika Merisca Gayatri Ryosa Meska Amelia Putri Miftah Irrahmah Miftah Irramah Murniwati Murniwati Mutya Restu Ayu Nila Kasuma Nining Kurniawati Nurhayati Nurhayati Nursyafi, Fauzan Syarif Prasetio, Ade Prima Astuti Handayani Prima Julistia Pritasa Muthia Ulfa Putri Mira Magistri Putri Mulya Sari Putri, Biomechy Oktomalio Rahmatini . Raihan Afif Salam Ramadhan, Mario Arya Ramadhan, Mario Arya Rauza Sukma Rita Reza, Mohamad Rikarni Rikarni Rima Semiarty Risa Firka Rismawati Yaswir Roslaili Rasyid Rydwan Efendi Saptino Miro Saptino Miro, Saptino Selfi Renita Rusjdi Sheila Jihan Safira Sofina Rusdan Suci Erawati Surya Nelis Susi Susi Susila Sastri Tafdil, Zhafirah Khairunnisa Yenny Mayang Sari Yulia, Dwi Yusri Dianne Jurnalis Yusri, Elfira