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Journal : Jurnal Penelitian Pendidikan IPA (JPPIPA)

Antibacterial Activity Test of Tembelekan Leaf Extract (Lantana camara linn) against Edwardsiella tarda bacteria Marwulan Marwulan; Happy Nursyam; Yuni Kilawati
Jurnal Penelitian Pendidikan IPA Vol 9 No 5 (2023): May
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i5.3459

Abstract

The purpose of this study was to analyze the optimal dose of Tembelekan leaf extract (Lantana camara Linn) which has antibacterial properties in inhibiting or killing Edwardsiella tarda bacteria. The method used in this research is Completely Randomized Design (CRD). The results of the research yield obtained from tembelakan leaf extract amounted to 8.9%. The positive phytochemical test results contained active compounds, namely flavonoids, alkaloids, tannins, triterpenoids, and saponins. GC MS analysis of tembelekan leaf extract showed that the most components were 4H-1-Benzopyran-4-one, 5,7-dihydroxy-6-methoxy-2-(4-methoxyphenyl)-,2,1,3-Benzothiadiazole, Benzisot hiadiazole , 3,4-Benzo-1,2,5-thia diazole. This compound is a class of isoflavonoids compounds. The MIC (Minimum Inhibitory Concentration) test results obtained the lowest absorbance value at a concentration of 100 mg/L with a value of 0.602 close to the positive control. Results of discs with doses of 50, 100, 150, 200, and 250 mg/L. The disc results showed that the dose of 200 mg/L had the strongest inhibition zone with a value of 12.63 mm. Tembelekan leaf extract which contains dominant compounds such as flavonoids and alkaloids can be useful as an antibacterial which inhibits the growth of E. tarda bacteria
Analysis of Water Quality and Phagocytic Activity (Clarias Sp.) in Aquaponic and Non-Aquaponic Cultivation Systems Punto Apri Sembodo; Asus Maizar Suryanto Hertika; Yuni Kilawati
Jurnal Penelitian Pendidikan IPA Vol. 9 No. 10 (2023): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i10.4295

Abstract

Catfish (Clarias sp.) is one of the many fishery biota that has animal protein which is much loved by Indonesian people. One way to develop environmentally friendly catfish cultivation is through the implementation of an aquaponic biofilter system. This research aims to analyze physical, chemical, and biological water quality parameters in catfish cultivation with and without aquaponics, to obtain a description of the non-specific immune system which is analyzed from the conditions of phagocytic activity of catfish cultivated with and without aquaponics. The method used is by taking samples ten times per week by taking water quality parameters as well as blood samples of catfish raised in ponds with and without aquaponics. The analysis used is the unpaired T-test. Based on the results obtained, it was concluded that all parameters showed that the aquaponic cultivation media was better compared to those without aquaponics.
Analysis of Protein Profile and Functional Groups in Biofilms in the Hypersaline Environment Ilham Misbakudin Al Zamzami; Yuni Kilawati; Raudatul Ibdiah; Andi Kurniawan
Jurnal Penelitian Pendidikan IPA Vol. 9 No. 12 (2023): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i12.4464

Abstract

Halophilic microorganisms include extremophiles that can live and develop in a hypersaline environment. Biofilm, as microecology, consists of a collection of microorganisms attached to a surface, forming an Extracellular Polymeric Substance (EPS) matrix. This study aims to analyze the protein profile and functional groups of biofilms that grow in hypersaline environments. The method used in this study is the SDS PAGE test to diagnose protein profiling and FTIR to analyze biofilm functional groups. The samples used to grow the biofilm came from salt ponds with different NaCl levels, namely 2%, 25%, and 40%. The results showed that each NaCl treatment had different functional groups in the biofilm samples. In the 2% NaCl treatment, there were peaks with solid intensity at the wavenumber 1100 cm-1, indicating the carboxylic acid functional group. In the 25% NaCl treatment, peaks with solid intensity at wavenumber 2250 cm-1 indicate the isocyanate functional group. In the 40% NaCl treatment, there were peaks with solid intensity at wavenumber 860 cm-1, indicating the alkene functional group. The protein analysis for each treatment shows the presence of pyruvate kinase protein in the biofilm with 2% NaCl. The 25% NaCl biofilm sample contained avidin protein, and the 40% NaCl biofilm sample contained tyrosine protein.
Analysis of Water Quality and Phagocytic Activity (Clarias Sp.) in Aquaponic and Non-Aquaponic Cultivation Systems Sembodo, Punto Apri; Hertika, Asus Maizar Suryanto; Kilawati, Yuni
Jurnal Penelitian Pendidikan IPA Vol 9 No 10 (2023): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i10.4295

Abstract

Catfish (Clarias sp.) is one of the many fishery biota that has animal protein which is much loved by Indonesian people. One way to develop environmentally friendly catfish cultivation is through the implementation of an aquaponic biofilter system. This research aims to analyze physical, chemical, and biological water quality parameters in catfish cultivation with and without aquaponics, to obtain a description of the non-specific immune system which is analyzed from the conditions of phagocytic activity of catfish cultivated with and without aquaponics. The method used is by taking samples ten times per week by taking water quality parameters as well as blood samples of catfish raised in ponds with and without aquaponics. The analysis used is the unpaired T-test. Based on the results obtained, it was concluded that all parameters showed that the aquaponic cultivation media was better compared to those without aquaponics.
Analysis of Protein Profile and Functional Groups in Biofilms in the Hypersaline Environment Zamzami, Ilham Misbakudin Al; Kilawati, Yuni; Ibdiah, Raudatul; Kurniawan, Andi
Jurnal Penelitian Pendidikan IPA Vol 9 No 12 (2023): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i12.4464

Abstract

Halophilic microorganisms include extremophiles that can live and develop in a hypersaline environment. Biofilm, as microecology, consists of a collection of microorganisms attached to a surface, forming an Extracellular Polymeric Substance (EPS) matrix. This study aims to analyze the protein profile and functional groups of biofilms that grow in hypersaline environments. The method used in this study is the SDS PAGE test to diagnose protein profiling and FTIR to analyze biofilm functional groups. The samples used to grow the biofilm came from salt ponds with different NaCl levels, namely 2%, 25%, and 40%. The results showed that each NaCl treatment had different functional groups in the biofilm samples. In the 2% NaCl treatment, there were peaks with solid intensity at the wavenumber 1100 cm-1, indicating the carboxylic acid functional group. In the 25% NaCl treatment, peaks with solid intensity at wavenumber 2250 cm-1 indicate the isocyanate functional group. In the 40% NaCl treatment, there were peaks with solid intensity at wavenumber 860 cm-1, indicating the alkene functional group. The protein analysis for each treatment shows the presence of pyruvate kinase protein in the biofilm with 2% NaCl. The 25% NaCl biofilm sample contained avidin protein, and the 40% NaCl biofilm sample contained tyrosine protein.
Identifikasi dan Analisis Molekuler Hemosianin sebagai Gen Ketahanan Tubuh Udang Vaname (Litopenaeus vannamei) Amanda, Talitha; Kilawati, Yuni; Maftuch
Jurnal Penelitian Pendidikan IPA Vol 10 No 6 (2024): June
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i6.7331

Abstract

Production of white shrimp (Litopenaeus vannamei) tends to decrease every year caused by diseases, one of which is White Feces Disease (WFD). Genetically, monitoring has been carried out on the parent characters of SPF (Specific Pathogen Free) shrimp imported from Hawaii and Florida with low genetic variation. If there is a threat such as disease caused by bacteria and the gene that codes for disease control or the body's resistance gene is missing, it allows the individual to become susceptible to disease. Gene that codes for body resistance in white shrimp is hemocyanin. Hemocyanin gene studies are needed as a first step in controlling disease in white shrimp. Method used is a descriptive method. Procedures carried out are sample collection, detection of WFD, molecular analysis of hemocyanin and data analysis. Results showed that healthy shrimp had hemocyanin while shrimp with WFD had very little or no amount of hemocyanin. Hemocyanin gene profile in healthy shrimp was also analyzed and there were 4 nucleotide change made changes to 4 amino acid. Changes in amino acids affect the stability of hemocyanin and cause polymorphism of hemocyanin. Phylogenetic analysis showed dendogram split into two clades between L. vannamei and P. monodon.
Fraction and Antibacterial Activity of Bitter Melons (Momordica charantia) in Koi Fish (Cypinus carpio) Infected with Aeromonas salmonicida Bacteria and its Effect on Gills Histopathology Nurandi, Nadiah; Andayani, Sri; Kilawati, Yuni
Jurnal Penelitian Pendidikan IPA Vol 10 No 12 (2024): December
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i12.9338

Abstract

Koi fish (Cyprinus carpio) is a type of freshwater ornamental fish with significant economic value, attracting widespread public interest due to its vibrant colors and agile movements. Therefore, this study introduces a novel approach by evaluating the antibacterial activity of bitter melon extract fraction (Momordica charantia) against A. salmonicida infection in koi fish and its impact on gill histopathology. The novelty of this study lies in the exploration of bitter melon extract fraction as a natural, eco-friendly antibacterial alternative to synthetic antibiotics in aquaculture. This research is crucial as it addresses the growing global concern over antibiotic resistance and the urgent need for sustainable, natural solutions in fish health management. Antibacterial tests were conducted to assess the ability of the bitter melon extract to inhibit bacterial growth, while histopathological analysis aimed to observe the specific changes in gill tissues of infected koi fish. The results revealed that the bitter melon extract fraction significantly reduced bacterial count and improved gill tissue integrity. Histopathological improvements were observed, including reduced hyperplasia, lamellar fusion, edema, and necrosis, indicating tissue recovery. This finding highlights the potential of bitter melon extract fraction as a natural antibacterial agent for controlling bacterial infections in fish, offering an alternative to chemical antibiotics. Further research is needed to determine the optimal dosage and to explore its antibacterial mechanism, ensuring its safe and effective application in aquaculture.
Co-Authors Abdul Rahem Faqih Adharul Muttaqin Adhitya Bhawiyuga Agoes Soeprijanto, Agoes Agum Bayu Gumelar Alif Raditya Amirul Huda Amanda, Talitha Andi Kurniawan Andi Kurniawan Anggita Noer Laily Aprillia Mifthakun Ni’mah Mifthakun Ni’mah Ni’mah Arning W. Ekawati Asus Maizar Suryanto H Asus Maizar Suryanto Hertika Attabik Muhammad Amrillah Attabik Mukhammad Amrillah Ayu Winna Ramadhani Dany Primanita Kartikasari Dany Primanita Kartikasari Dany Primanita Primanita Kartikasari Devi Rahmania Nureka Wasti Dewi Wulandari Diana Aisyah Diana Aisyah Diana Arfiati Dimas Adetia Rikianto Dini Anggraini Endang Yuli Herawati Fahma, Yunita Rifda Alano Fitri Sil Valen Fitri Sil Valen Hafish Gigih Purnama Happy Nursyam Haryono, M. Gandri Herwati Umi Subarijanti Ibdiah, Raudatul Ilham Afandy Afandy Ilham Misbakudin Al Zamzami Islamy, R Adharyan Isroni, Wahyu Iswati Iswati Lini Murni Maftuch Maftuch Maftuch Maftuch Maftuch Maheno Sri Widodo Maheno Sri Widodo, Maheno Sri Marwulan Marwulan Moh. Awaludin Adam MOHAMAD FADJAR Mohamad Fadjar Mohamad Fadjar Mohammad Mahmudi Muhammad Musa Muhammad Nafar Amani Syams Mulyanto, Febri Dwi Novalina Serdiati Nurandi, Nadiah Nurul Mutmainnah, Nurul Pramudia, Zulkisam Punto Apri Sembodo Punto Apri Sembodo Putri, Ardyah R.I. R Adharyan Islamy Raudatul Ibdiah Rizqa, Vylzah Saidah Luthfiyah Sembodo, Punto Apri Sholikah, Lik Anatus Sisca Fajriani Siti Nur Tahirah Sri Andayani Sri Widyarti Sugiarto, Kevin Pratama Suliswanto Suliswanto Susanti, Yogita Ayu Dwi Venny Nur Hidayah Veryl Hasan Win Darmanto Yatris Rambu Tega Yenny Risjani Yuliezar Perwira Dara, Yuliezar Perwira Yunita Maimunah Zahrotur Rosyidah Zamzami, Ilham Misbakudin Al