Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : Aquasains : Jurnal Ilmu Perikanan dan Sumberdaya Perairan

ASSAY OF Acinetobacter AS COMPETITOR TO VIBRIOSIS LUMINESCENCE BACTERIA IN PACIFIC PACIFIC WHITE SHRIMP(Litopenaeus vannamei) Assovaria .; Esti Harpeni; Asep Saefulloh
AQUASAINS Vol 4, No 1 (2015)
Publisher : Jurusan Perikanan dan Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (519.196 KB)

Abstract

Pacific white shrimpculture is having problems caused by bacteria infection. The cause of  bacteria infection is V. harveyi, it can decrease seed production. Pathogenicity of vibriosis can decrease with inhibition by other types of  bacteria. The aim of the research was to get competitor V.harveyi isolates through identification and challenge test in vitro and in vivo. The result of screening of bacterial isolates were obtained from cultivation supplement products to produced 4 pure isolates with different morphologically colonies and cells. There was one potential isolate could inhibits V. harveyi by inhibition test in vitro. Biochemical test from three different  laboratories were identified that Acinetobacter sp. as competitor to V. harveyi and it was not pathogenic to Pacific white shrimp. Challenge test Acinetobactersp. against V. harveyi on Pacific white shrimp larvae was conducted through three treatments, without given V.harveyi and Acinetobacter sp. As controls; treatment with given bacteria V.harveyi and treatment with given V. harveyi and Acinetobacter sp. The challenge test results showed that treatment with given of V.harveyi and Acinetobacter sp. may decreased V. harveyi density of 6.3x106CFU/mL to 7.5x103CFU/mL which could suspected to decrease pathogenicity of competition number of colonies.
Suplementasi Minyak Ikan Untuk Peningkatan Imunitas Non Spesifik Udang Vaname (Litopenaeus vannamei) Vina Olivia Indraswati; Supono Supono; Asep Saefulloh
AQUASAINS Vol 3, No 2 (2015)
Publisher : Jurusan Perikanan dan Kelautan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (385.615 KB)

Abstract

Increased immunity shrimp became one of the targets of feeding with a complete nutrient constituent composition. Shrimp have a different immune system compared to fish in general and can be increased quickly by providing the right nutrients. Research nutrient supplementation to increase non-specific immunity, especially in Pacific white shrimp (Litopenaeus vannamei) still rarely done, especially with the addition of fish oil. This research was conducted to study the effect of fish oil supplementation in different oil among lemuru oil and patin oil with a non-specific parameter of Pacific white shrimp among total haemocyte count (THC), the differential haemocyte count (DHC), challenge test with Vibrio harveyi and relative percent survival (RPS). Design of experiments using a completely random design with three treatments and four replications. The treatment is adding  the lemuru oil (treatment A), patin oil (treatent B) and without the addition of fish oil (control). The results showed that the addition of lemuru fish and patin fish oil treatment can increase THC, DHC, which affect the phagocytic activity relative to the level of protection without giving fish oil (P<0.05). The increasing of non-specific immunity of Pacific white shrimp with fish oil needs to analyze in the economic  side to be more economical if applied to the shrimp farmers or commercial feed mills.