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Journal : Jurnal Riset Rumpun Ilmu Kesehatan (JURRIKES)

Potensi Fungi Endofit dari Daun Sirih (Piper Betle L.) sebagai Alternative Pencarian Senyawa Antibiotik terhadap Bakteri Penyebab Infeksi Marhamah Marhamah; Muh Danial Fajri; Lola Tulak Rerung
JURNAL RISET RUMPUN ILMU KESEHATAN Vol. 4 No. 2 (2025): Agustus : Jurnal Riset Rumpun Ilmu Kesehatan
Publisher : Pusat riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/jurrikes.v4i2.5718

Abstract

Infections and antibiotic resistance pose significant challenges to global healthcare. This study aims to evaluate the antibacterial potential of endophytic fungi isolated from betel leaves (Piper betle L.) against Staphylococcus aureus and Staphylococcus epidermidis. The research involved isolating endophytic fungi using Potato Dextrose Agar (PDA), purifying the isolates, and testing their antibacterial activity through the disk diffusion method. The results revealed that three endophytic fungal isolates produced inhibition zones, with the highest average diameter of 17.46 mm against Staphylococcus aureus and 18.66 mm against Staphylococcus epidermidis. These findings indicate the potential of betel leaf endophytic fungi as a natural source of antibacterial agents. This study underscores the role of endophytic fungi in developing new antibiotics to combat bacterial resistance.
Potensi Fungi Endofit Daun Sambung Nyawa (Gynura Procumbens) sebagai Alternatif Antibakteri Baru Muh Danial Fajri; Djulfikri Mewar; Marhamah Marhamah
JURNAL RISET RUMPUN ILMU KESEHATAN Vol. 4 No. 2 (2025): Agustus : Jurnal Riset Rumpun Ilmu Kesehatan
Publisher : Pusat riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/jurrikes.v4i2.5804

Abstract

The Sambung Nyawa plant (Gynura procumbens) is known to have various benefits, including as an antibacterial. This study aims to identify and evaluate the antibacterial properties of secondary metabolites from endophytic fungi isolated from Sambung Nyawa leaves against Staphylococcus epidermidis and Staphylococcus aureus bacteria. Fungi were isolated and then fermented for 7 days using Nutrient Broth media. Antibacterial activity was evaluated using agar diffusion technique, with zone of inhibition as an indicator of activity. The results showed that IFDSN 2 fungal isolate had the highest zone of inhibition, with a very strong category against S. aureus and strong against S. epidermidis. This finding supports the potential of endophytic fungi as an environmentally friendly natural antibacterial source.