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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) Jurnal Ilmu Pertanian Jurnal Keteknikan Pertanian Jurnal Hortikultura Jurnal Pengkajian dan Pengembangan Teknologi Pertanian Jurnal Penelitian Tanaman Industri AGRIVITA, Journal of Agricultural Science Buletin Kebun Raya agriTECH Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat EnviroScienteae Jurnal Penelitian Pertanian Tanaman Pangan ANNALES BOGORIENSES Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) JURNAL PERTANIAN PRESISI (JOURNAL OF PRECISION AGRICULTURE) Journal of Tropical Crop Science JURNAL PERTANIAN jurnal Ilmu Pertanian (Agricultural Science) ZIRAA'AH MAJALAH ILMIAH PERTANIAN Jurnal Penelitian Tanaman Industri (Littri) Statement : Jurnal Media Informasi Sosial dan Pendidikan El Dusturie: Jurnal Hukum dan Perundang-undangan Jurnal Tarbiyah Jamiat Kheir Annales Bogorienses
Slamet Susanto
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural University), Jl. Meranti, Kampus IPB Darmaga, Bogor 16680, Indonesia
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Journal : Annales Bogorienses

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In Vitro Induction of Tetraploid Pummelo ’Nambangan’ (Citrus maxima (Burm.) Merr.) by Colchicine Treatment Using Germinated Seed, Shoot Tip and Cotyledonary Node As Explants Wulandari, Dyah Retno; Purwito, Agus; Susanto, Slamet; Husni, Ali; Ermayanti, Tri Muji
Annales Bogorienses Vol. 19 No. 1 (2015): Annales Bogorienses
Publisher : BRIN

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Abstract

Tetraploid citrus are important for interploidal hybridization to create triploid seedless citrus. Colchicine is the most commonly used as antimitotic agent to induce polyploid plants. Tetraploid induction by colchicine in Pummelo ‘Nambangan’ was conducted in vitro using different types of explants. The aim of this research was to induce tetraploid pummelo ‘Nambangan’ by colchicine treatment using germinated seed, shoot tip and cotyledonary node as explants. Tetraploid shoot induction was conducted by soaking germinated seeds, shoot tips and cotyledonary nodes in 0.1 % colchicine for 1, 3 and 5 hours. Regenerant shoots were grown on MS medium and their growth was observed after four weeks in culture. Ploidy level was determined using flow cytometry analysis. Stomata density, length and width of stomatal guard cell were also recorded. The results showed that shoot elongation was inhibited by colchicine treatment. Soaking of shoot tip explants in 0.1 % colchicine for 1 hour resulted in 66.66 % of putative tetraploid shoots. Compared to diploid shoots, tetraploids had lower stomata density but bigger in guard cell size.
Co-Authors , Hariyadi , Hasnam , Mukhlas , Sakhidin , Setyono . Santosa . Saputera . Strisno . Sutrisno A. S. Abidin Abdullah Bin Arif Abdullah Bin Arif, Abdullah Agus Purwito AHMAD JUNAEDI Ahmad Junaedi Ahmad S. Abidin Aji, Titistyas Gusti Ali Husni Ali Husni Ali Husni Ali Husni Amin Rejo Anas Dinurrohman Susila Ani Kurniawati Antik Siti Latifah Arifah Rahayu Asniwita Asniwita Atika Romalasari Bambang B. Santoso Bambang Budi Santoso Bambang Pramudya Bambang Pramudya Bambang S . Purwoko Bambang S. Purwoko Bambang S. Purwoko Bambang Sapta Purwoko Bhayu Hartanti DADANG DADANG Dadang Hermansyah Dadang Hermansyah Delys Inkorisa Dewi Sukma Dewi, Iswari Saraswati Dhika Prita Hapsari DJUMALI DJUMALI DJUMALI DJUMALI, DJUMALI Dona Rustani DWI ANDREAS SANTOSA Dyah Retno Wulandari Dyah Retno Wulandari Edi Minaji Pribadi Edi Santosa Efendi, Darda Endro Priherdityo Erniawati Diningsih Erniawati Diningsih Erniawati Diningsih Evan Yonda Pratama Faqih Udin Fidya Novita Fiki, Ainun Gede Suastika Gede Suastika GEDE SUASTIKA Gede Suastika Giyanto, Giyanto Habibi, Irfan Hadi K. Purwadaria Handian Purwawangsa Hariyadi Hariyadi Hariyadi3, , Harliani Sri Utami Henny Nurpa Anggriani Herik Sugeru Hermansyah, Dadang Hilda Susanti Hulu, Versi Putra Jaya I Wayan Budiastra Indriati Husain Iswari S Dewi Iswari S. Dewi Iswari Saraswati Dewi Jamhari Jamhari Karmanah, Karmanah Kartika Ning Tyas Kartika Ning Tyas Ketty Suketi Kikin H Mutaqin Kosmaryadi, Nandi Kristriandiny, Oktiadewi Kudang B Seminar Kudang B. Seminar Kurniawan, Nafi’ Leo Mualim Lia Rachmawati M. Wahyudin Nasrulloh, M. Wahyudin Nasrulloh Matra, Deden Derajat Maya Dewi Sulistyningrum Maya Melati Moeljarno Djojomartono Moeljarno Djojomartono Moh Nailun Ni'am MOHAMMAD CHOLID MOHAMMAD CHOLID, MOHAMMAD Muhamad Ramdan, Muhamad Muhammad Syukur Muhammad Thamrin Natalia, Cristina Evi Nawawi, Muhammad Adlan Neni Musyarofah Nurul Khumaida Nurul Khumaida Nurul Khumaida Oktiadewi Kristriandiny Parameswara, Yosephine Sista Pratama, Evan Yonda Priherdityo, Endro Purwoko, Bambang Sapto R. Poerwanto Raden Ajeng Diana Widyastuti Rahayu, Arifah Resti Putri Septyani Resti Putri Septyani Retty Nurfazizah Roedhy Poerwanto Roedy Poerwanto Rumaisha, Azizah Rustam, Rustam Rustani, Dona Sakhidin Sakhidin Sandra A. Aziz Sandra Arifin Azis Sandra Arifin Aziz Santosa Santosa Sintho Wahyuning Ardie Siregar, Shella Elvira Siti Aisyah Rohmatus Sa’adah SRI HENDRASTUTI HIDAYAT Sri Minten Sriani Sujiprihati Sudirman Yahya Sukma, Dewi Suroso Suroso Suryo Wiyono Susanto, Renaldy Sutrisno Sutrisno Sutrisno Sutrisno Suyanto Kartosoewarno Thamara, Aria Tirtawinata, Mohamad Reza Titistyas Gusti Aji TRI ASMIRA DAMAYANTI Tri Muji Ermayanti Tri Muji Ermayanti Tri Muji Ermayanti Ummu Kalsum Ummu Kalsum Wahyu Fikrinda Widayanti, Siti Mariana Widyaswara, Muhammad Heraldi Garda Willy Bayuardi Suwarno Winarso D. Widodo Wulandari, Dyah Retno Wulandari, Dyah Retno Yosephine Sista Parameswara Yudi Chadirin Yul Y Nazaruddin