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All Journal ACTA VETERINARIA INDONESIANA Jurnal Natural Jurnal Kedokteran Hewan Jurnal Media Tropika: Jurnal Pengabdian Kepada Masyarakat Peternakan Abdi Masyarakat (PETAMAS)
Muhammad Jalaluddin, Muhammad
Laboratorium Anatomi Fakultas Kedokteran Hewan Universitas Syiah Kuala, Banda Aceh
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Veterinary

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Journal : Jurnal Kedokteran Hewan

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MICROSTRUCTURAL CHARACTERISTICS OF THE Longissimus dorsi MUSCLE IN BRAHMAN CROSS CATTLE Sofyan, Hamny; Jalaluddin, Muhammad; Rahmi, Erdiansyah; Wahyuni, Sri; Hidayat, Taufan; Adam, Mulyadi; Gani, Fadli A; Sabri, Mustafa; Ritonga, Mudhita Zikkrullah
Jurnal Kedokteran Hewan Vol 16, No 2 (2022): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v16i2.25885

Abstract

This study aimed to analyze the muscle microstructure of longissimus dorsi Brahman cross (BX) cattle slaughtered at the slaughterhouse of North Aceh District. The meat samples for the study were taken from three male BX cattle aged 18-30 months with body weights ranging from 400 kg to 500 kg and the average body condition scores (BCS) of 3-4. The muscle used was the longissimus dorsi muscle. The mean (SE) diameter and cross-sectional area (CSA) of muscle fibers and the percentage of fast fibers in the longissimus dorsi BX muscle were 71.691.23 m, 4263.43305.69 m2 , and 48.178.81%, respectively. This study concluded that the longissimus dorsi muscle of the BX has relatively large muscle fibers, some of which in each fascicle are composed of fast muscle (fast fiber/type II).
CHARACTERIZATION OF GLYCOCUNJUGATE DISTRIBUTION IN THE EPIDIDYMIS OF THE JAVAN MUNTJACS (Muntiacus muntjak muntjak) Wahyuni, Sri; Sofyan, Hamny; Hafizuddin, Hafizuddin; Adnyane, I Ketut Mudite; Gholib, Gholib; Jalaluddin, Muhammad; Gani, Fadli A.; Adam, Mulyadi; Siregar, Tongku Nizwan; Agungpriyono, Srihadi
Jurnal Kedokteran Hewan Vol 16, No 1 (2022): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v16i1.24418

Abstract

The presence of glycoconjugates in caput, corpus, and cauda epididymis of the Javan muntjacs was performed using lectin histochemical method. The epididymis organs were obtained from two adult male Javan muntjacs in hard antler stage and then immediately fixed in Bouins solution and processed in histological manner. Six types of biotinylated lectins namely Peanut agglutinin (PNA), Soybean agglutinin (SBA), Wheat germ agglutinin (WGA), Ricinus communis agglutinin (RCA), Concanavalin A (Con A), and Ulex europaeus agglutinin I (UEA I) were applied to detect the specific glycoconjugates in the epididymal tissue of muntjacs. The result showed the presence of glycoconjugates were detected by five types of lectins except UEA I in caput, corpus, and cauda epididymis with specific distribution and also the intensity of lectins binding. Glycoconjugates -galactose, -glucose, mannose, N-acetyl-galactosamine, N-acetyl-glucosamine, and sialic acid were stained with various intensity in epithelial cells, basal cells, stereocilia, spermatozoa, and stroma of caput, corpus, and cauda epididymis. The presence of sialic acid on the surface of spermatozoa detected by WGA are thought to be play a role on sperm protection during their maturation and transit along epididymal lumen. In conclusion, the difference of glycoconjugates distribution pattern and lectin binding in the epididymal caput, corpus, and cauda of the Javan muntjac indicates the regional differences in epididymal function in spermatozoa maturation.
STUDI HISTOKIMIA LEKTIN PADA SEL-SEL SPERMATOGENIK TESTIS MUNCAK (Muntiacus muntjak muntjak) (Lectin Histochemical Study of Testicular Spermatogenic Cells in Muntjak (Muntiacus muntjak muntjak)) Wahyuni, Sri; Agungpriyono, Srihadi; Adnyane, I. Ketut Mudite; Hamny, Hamny; Jalaluddin, Muhammad; Gholib, Gholib; Akmal, Muslim; Adam, Mulyadi; Aliza, Dwinna; Siregar, Tongku Nizwan
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i1.3396

Abstract

The objective of this study was to identify the type of specific glycoconjugates and its distribution in testicular spermatogenic cells in muntjak (Muntiacus muntjak muntjak) based on lectins histochemistry. An adult male muntjak aged 4-5 years old in hard antler period was used in this study. Testicular tissue was fixed in Bouin solution and processed histologically. Histochemistry method was performed using six types biotinylated lectins such as peanut agglutinin (PNA), soybean agglutinin (SBA), wheat germ agglutinin (WGA), ricinus communis agglutinin (RCA), concanavalin A (Con A), and ulex europaeus agglutinin I (UEA I) with 20 g/ml of concentration for PNA lectins and 15g/ml for other type of lectins. The results showed that glycoconjugates were detected by all type of lectins except UEA I in testicular spermatogenic cells with variation in distribution pattern and also the intensity of lectins binding. Glycoconjugates -galactose, -glucose, mannose, Nacetylgalactosamine, N-acetylglucosamine and sialic acid were stained intensely by lectins in golgy-cap phase and acrosomal phase of spermatids. Glycoconjugate N-acetylgalactosamine was the sugar residues which distributed abundantly that marked by positive reaction with PNA, SBA, and RCA lectins. In conclusion, glycoconjugates are detected in testicular spermatids cells of muntjak indicated that glycoconjugates have an important role in spermatogenesis particularly in spermiogenesis.Key words: glycoconjugates, lectins, spermatid, spermatozoa, muntjak
Co-Authors ., Daniel Ali, Ihfan Khaidir AlKautsar, AlKautsar Andi Novita Daniel Daniel Dwinna Aliza Erdiansyah Rahmi Fahrezi, Muhammad Gilbran Faisal Mustafa Frengki Frengki, Frengki Gani, Fadli A Gani, Fadli A. Gholib Gholib, Gholib Hafizuddin Hafizuddin Hamny Hamny Hamny Sofyan Hasriati Hasriati, Hasriati I. Ketut Mudite Adnyane, I. Ketut Mudite Idham Khalid Ilham Ilham Isky, Saiful Ketut Adnyane Mudite Kusdiantoro Mohamad Makmur, Ali Mario Pani Mulyadi Adam, Mulyadi Muslim Akmal Noni Zakiah Rasyida Ulfa Riandi, Lian Varis Ridhana, Fita Ritonga, Mudhita Zikkrullah Ritonga, Mudhita Zikrullah Rosmaidar Rosmaidar, Rosmaidar Sari, Rahayu Eka Sari, Wahyu Eka Sri Wahyuni Srihadi Agungpriyono Tanjung, Fitri Aisah Taufan Hidayat Tongku Nizwan Siregar Wahono Esthi Prasetyaningtyas Wardhani, Lailia Dwi Kusuma Yulisma, Rika