Article Per Year (5 Year)
p-Index From 2020 - 2025
2.501
P-Index
This Author published in this journals
All Journal Bioma : Berkala Ilmiah Biologi Jurnal Ilmu Lingkungan Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Biosfer: Jurnal Tadris Biologi Jurnal Biologi Tropis Jurnal Biogenerasi BIOVALENTIA: Biological Research Journal Jurnal Kedokteran Hewan Journal Of Agriculture Sciences (Agrosci)
Eko Agus Srihanto, Eko Agus
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Social Sciences Veterinary Other

Published : 16 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search
Journal : Journal Of Agriculture Sciences (Agrosci)

 1 
Molecular Detection of Jembrana Disease in Balinese Cattle (Bos sondaicus) Samples at the Lampung Veterinary Center Kurniasih, Vidyanti; Rustiati, Elly Lestari; Srihanto, Eko Agus; Firwantoni, Firwantoni; Heni, Ahyul; Panasea Anggy, Fransiska; Febriyani, Dwi Ayu
Jurnal Agrosci Vol 2 No 3 (2025): Vol 2 No 3 January 2025
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/agrosci.v2i3.578

Abstract

Jembrana Disease is an infectious disease in Balinese cattle, which is characterized by fever and inflammation of the oral mucous membranes (stomatitis, enlarged lymph nodes of the preskapularis, prefemolaric, and parotid, sometimes accompanied by blood sweating. This disease reaches a mortality rate of no more than 20% caused by the Jembrana Disease Virus (JDV). According to data from the Directorate of Animal Health, Jembraa Disease cases continue to increase from year to year, with as many as 398 cases in 2016 and increased to 4,797 cases in 2018. The presence of the Jembrana virus can be detected through tests. One of them is by identifying and isolating the pathogen. Technological breakthroughs in DNA-based testing systems are becoming an available alternative. Viruses can be detected through commonly used molecular analysis such as Polymerase Chain reaction (PCR). The Jembrana Disease test was carried out at the Biotechnology Laboratory of the Lampung Veterinary Center using PCR and then visualized by agarose gel electrophoresis. The test results of 53 samples consisting of 50 types of buffycoat samples and 3 types of organ samples showed that 1 type of organ sample was detected with JDV
Molecular Detection of Lumpy Skin Disease in Cattle Samples (Bos taurus) at Lampung Disease Investigation Center Yunior, Elfita Nova; Rustiati, Elly Lestari; Srihanto, Eko Agus; Firwantoni, Firwantoni; Heni, Ahyul; Panasea Anggy, Fransiska; Febriyani, Dwi Ayu
Jurnal Agrosci Vol 2 No 4 (2025): Vol 2 No 4 March 2025
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/agrosci.v2i4.640

Abstract

Lumpy Skin Disaese, LSD, was first discovered in Indonesia in Riau Province in February 2022. Although LSD is not a zoonotic disease, its infection can affect the production and reproductive performance of cattle, resulting in various impacts including reduced milk production, weight loss, miscarriages, and infertility as well as significant economic losses. It is a vector-borne smallpox disease of cattle and buffalo. Characterized by the appearance of pustules on the skin. Identification of LSDV can be carried out by three methods, namely Polymerase Chain Reaction (PCR), virus isolation, and electron microscopy. PCR is the cheapest and fastest method to detect LSDV. LSD disease testing at the Biotechnology Laboratory, Lampung Disease Investigation Center using the RT- PCR method. No LSD was found over 13 blood samples and 2 nasal swab samples from January 2024 sampling showed that all of them were negative to LSD.
Sex Determination on Racing Pigeons (Columba livia) Molecularly Using Blood Samples Azzahra, Laila Salwa; Srihanto, Eko Agus; Rustiati, Elly Lestari; Master, Jani; Firwantoni, Firwantoni; Febriyani, Dwi Ayu; Pratiwi, Dian Neli
Jurnal Agrosci Vol 2 No 6 (2025): Vol 2 No 6 July 2025
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/agrosci.v2i6.785

Abstract

Background. Pigeons (Columba livia) belong to the Columbidae. Pigeons possess diverse ecological, economic, and aesthetic values, as they are monomorphic, exhibiting similar characteristics between male and female individuals, especially at a young age. Aims. In collaboration with the Lampung Disease Investigation Center, a study has been done to confirm the sex determination of pigeons at a young age by the PCR technique. Blood samples were taken in two locations, East Lampung and Kota Metro. Molecular análisis was carried out at the Biotechnology Laboratory, Lampung Disease Investigation Center. Methods. The technique includes DNA extraction, DNA amplification, electrophoresis and visualization. Result. It poses a significant challenge for pigeon breeders in sex determination. Blood is a source of genetic material that can be used to determine the sex of birds. The Chromo-Helicase-DNA-binding Protein (CHD) gene is one of the genes used as a marker to molecularly differentiate the sex of birds. The sex chromosomes in female individuals have heterozygous chromosomes (ZW) and male individuals have homozygous sex chromosomes (ZZ). Conclusion. Of eleven pigeon blood samples, there were seven female and four male individuals, and there was one mistake in the gender prediction by the pigeon owner
Sex Determination in Racing Pigeons (Columba livia) Molecularly Using Young Feather Samples Adelia, Ersa Imelda; Srihanto, Eko Agus; Rustiati, Elly Lestari; Master, Jani; Firwantoni, Firwantoni; Anggy, Fransiska Panasea; Neli Pratiwi, Dian
Jurnal Agrosci Vol 2 No 6 (2025): Vol 2 No 6 July 2025
Publisher : Ann Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62885/agrosci.v2i6.807

Abstract

The racing pigeon (Columba livia) has an aesthetic value and is in high demand. Pigeon is monomorphic, difficult to distinguish between male and female individuals, especially at the age of offspring. Knowing the sex of birds from an early age is necessary to prepare male bird individual as racing bird. Accurate determination of the sex of racing pigeons from an early age was carried out to have scientific confirmation of the molecular method of determining the sex in racing pigeon. Under Lampung Disease Investigation Center program, molecular methods of PCR techniques using primers 2550F and 2718R were applied to determine the sex of bird based on the presence of Z and W chromosomes from young feathers of racing pigeons. Sampling young feathers was done in individual breeders in Kota Metro and East Lampung. Molecular analysis was carried out at the Biotechnology Laboratory of the Lampung Disease Investigation Center, consisted of extraction, amplification, electrophoresis, and data analysis. Fifteen young feather samples showed five male individuals with electrophoresis visualization formed one band measuring 600 bp and ten female individuals showed two bands measuring 600 bp and 400 bp.
Co-Authors Adelia, Ersa Imelda Afandi, Aril Alfandi, Aril Anggy, Fransiska Panasea Ashari, Minanti Mayda Azzahra, Laila Salwa Elly Lestari Rustiati Fahrezi, Aditya Febriansyah, Muhammad Febriyani, Dwi Ayu Firwantoni, Firwantoni Gina Dania Pratami, Gina Dania Heni, Ahyul Imtitsal, Aulia Indah Ayu Lestari, Indah Ayu Jani Master, Jani Krismuniarti, Elisabeth Devi Kurniasih, Vidyanti Kusuma, Andriyani Wijaya Lestari, Septi Wahyu Maharani, Annisa Lidya Michael Haryadi Wibowo Neli Pratiwi, Dian Novianasari, Tika Panasea Anggy, Fransiska Permatasari, Nindy Pratiwi, Dian Neli Priyambodo Priyambodo Priyambodo Priyambodo Priyambodo Ramadan, Viki Ramadhan, Viki Rustiaati, Elly Lestari Sa'uddah, Lousanja D. Sandra, Shifa Saswiyanti, Enny Sidik, Mahfud Sri Wahyuningsih Suhada Suhada, Suhada Susanto, Alvin Wiwiet Thesalonika, Natasya Virnarenata, Elsa Widya Asmara WINARNO Winarno Winarno Yani, Ani Andri Yanti Yulianti Yuliana Andriyani Yunior, Elfita Nova