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Journal : Annales Bogorienses

Comparison of the Reduction Effect of Sucrose and Table Sugar Concentration on Growth Characteristics of Red Ginger (Zingiber officinale Rocs.) Cultured in Liquid Medium Hapsari, Betalini Widhi; Ermayanti, Tri Muji; Rantau, Deritha E.; Rudiyanto, Rudiyanto
Annales Bogorienses Vol. 15 No. 1 (2011): Annales Bogorienses
Publisher : BRIN

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The aim of this research was to compare the reduction effect of sucrose or table sugar concentration on growth characteristics of red ginger cultured in MS liquid medium. Shoots of red ginger was cultured on MS liquid medium without addition of plant growth regulators, supplemented with 5, 10, and 20 g/l of sucrose or table sugar for 8 weeks. Resulted plantlets were acclimatized in a greenhouse to investigate their growth and survival rate. Numbers of stomata, chlorophyll concentration as well as cross section of leaves from plantlets grown in vitro were compared to those of transplants grown in the greenhouse. The results showed that the use of table sugar at concentration of 20 g/l gave the best growth of red ginger. Meanwhile, the reduction of table sugar from 20 to 10 g/l reduced growth and survival rate of in vitro shoots as well as that of transplants in the greenhouse. Only few shoots formed roots when they were grown on the medium containing 5 g/l of table sugar, and transplants failed to grow in the greenhouse. It found that the chlorophyll content of in vitro plantlets was lower than those of transplants grown in the glasshouse. However, the number of stomata of the in vitro plantlets was higher than that of transplants grown in the glasshouse. There was no anatomical abnormalities found on the cross section of leaves between in vitro plantlets and transplants grown in the greenhouse. The replacement of sucrose with table sugar may reduce the production cost of plantlets.
Somatic Embryo Germination of Jatropha curcas L in Presence of Sucrose and Poly Ethylene Glycol (PEG) Rudiyanto, Rudiyanto; Efendi, Darda; Ermayanti, Tri Muji
Annales Bogorienses Vol. 18 No. 1 (2014): Annales Bogorienses
Publisher : BRIN

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Jatropha curcas L. is a potential source of a non-edible biofuel. Conventional propagation of J. curcas technique has some limitations. Somatic embryo can produce a large number of embryos and obtain a large number of plants all year round. Treatment of sucrose in combination with polyethylene glycol (PEG) was proven to enhance germination of somatic embryos in many plant species. The aim of the study was to investigate the effect of sucrose in combination of PEG on somatic embryo germination in J. curcas. Globular somatic embryos at 0.025-0.030 g fresh weight having 0.4-0.5 cm in diameter were grown on MS medium solidified with 3 g/l of Gelzan supplemented with sucrose at 20, 30, 40, and 50 g/l in combination with PEG at 0, 2.5, 5, 10, and 15%. Results showed that the best medium for germination of J. curcas somatic embryo cultures was MS medium supplemented with 20 and 30 g/l of sucrose in combination with 5% of PEG. The numbers of germinated embryos per clump had significant enhancement on those medium compared with the control (PEG free treatment) (2.65 to 5.65) and (2.55 to 5.50). In addition, those treatments resulted in the highest percentage of clumps forming germinated embryos (100%), with an average of normal germinated embryos at 94.163 and 96.065%. The addition of 40 and 50 g/l of sucrose in combination with 15% of PEG caused all embryos to fail at germinating.
Co-Authors Aan Listiana Abshoril Fithry Agus Anggoro Sigit Agus Putra Murdani Agustina, Mulya Al Hasan, M. Iqbal Ali Nurhadi Alif Noor Anna Amanda, Efina Anitarini, Fany Anshar Haryasakti, Anshar Ardi S, Muhamad Arifuddin, Yusuf Waliyyun Arini, Dewi Ulfah Ariska, Hanisa Iis Ariyani, Anita Dwi Arya, I Gusti Komang Asti Melani Astari Badriyah, Khoirina Balqis, Rani Diana Betty Erda Yoelianita Butar-Butar, Mangasi Calvin Calvin Caressa, Dea Amanda Chusnul Arif Cici Sapitri, Cici Damayanti, Fransiska Erna Devi Catur Winata Devianto, Dodi Dewi Novita Sari Dinawati, Yuli Dinawati Dwi Astuti Efendi, Darda EKO BUDIYANTO El Ayyubi, Rifqi Afif Eliyawati, Cucu Elvira Sari Dewi Eny Heriyati, Eny Ernawulan Syaodih Ervia Toga Euis Kurniati Faqih, Achmad Fardhani, Indra Faridawati, Ria Fatimah, Imas Fauziyah, Afina Lathifah Furoida, Khusna Gulo, Amos Fransiskus Gunawan, Renaldi Gustini, Dini Habiddin hanifah hanifah Hapsari, Betalini Widhi Hasannah, Annis Watun Hasibuan, Lilis Harianti Hendra Wijaya Heny Djoehaeni Herlina Herlina Hermanto, Andrik Hesniati, Hesniati Hidayat, Risma Wahyuni Hosnia, Hosnia Hutagaol, Jarunjung Iis Rodiah Indriani, Ninis Inggita Kusumastuty Intan Yusuf Habibie Ismail, Dina Dewi Sartika Lestari Istikomayanti, Yuswa Iswahyudi, Ulul Azmi Izzah, Ukhtul Joey, Joey Johny Budiman Jumadi Jumadi Juni Risma Saragih, Herlina Kaharuddin Karimah, Nadia Kawitantri, Orchidara Herning Koen Irianto Uripan Kristijarso, Kristijarso Kumboyono Kumboyono Kurniawan, Rubi Kusumawati, Diana Lafiya, Rifdatul Latifah, Julia Rahma Legowo, Endro LELI KURNIAWATI Lina Lina Lindawati Lindawati Lola Ayu Istifiani Maiyastri, Maiyastri Marsetio Marsetio Masroni Misrofingah, Misrofingah Mizoguchi, Masaru Moh. Zeinudin Mohammad Nizarul Alim, Mohammad Nizarul Mubiar Agustin Mudinillah, Adam Mufliha, Dewi Insani Muladefi Choiriyah Munawar Cholil Munawar, Niknik Ahmad Munif, Badrul Nazmi, Annisa Nur Nirmala, Dwiputri Nofrika Sari, Mira Novitasari, Destin Nur Faizah Romadona Nur Rohmania, Qonita Nur, Subandi Nuradhani, Dadan Nurhayati, Emilia Ocih Setiasih Oktapiani, Cica Sri Pamungkas, Akhmad Yanuar Fahmi Paniran Paniran Prakoso, Lukman Yudho Prasetyawan, Riyan Dwi Prayoga, Rahmat Priyono Priyono Pujo Widodo, Pujo Putri, Farah Rizkita Putri, Illene Nabila Rahmawan, Fajri Andi Rahmawati, Gina Maliha Rantau, Deritha E. Risma Juni Saragih, Herlina Rita Mariyana Rosdianto Rosdianto, Rosdianto Roshanti, Dian Rosiana, Angela Febiana Dewinta Rosnita, Astiani Rosuli, Ahmad Saleh, M. Firdaus Samvara, Made Adhiguna Saraswati, Meinarti Sari, Putri Indah Satrianto, Anang Sholihin Sholihin Sholihin Siti Aminah Siti Sofiah Suharjo Suharjo Sumosusilo, Joko Supriyanto Supriyanto Suryanto Suryanto Sutisna, Ade Jaya Sutomo Sutomo Sutoyo Sutoyo Suwarno, Panji Syawaluddin Syawaluddin, Syawaluddin TARYATMAN TARYATMAN Tasrief, Muhdar Timamah, Iik Titin Andri Wihastuti Toga, Erik Tri Muji Ermayanti Tunnufus, Zakiyya uni Risma Saragih, Herlina J Vidya Nahdhiyatul Fikriyah Viviani, Viviani WAHYU SETYANINGSIH Wahyudi, Muhammad Hirwan Widasari, Ela Yanuar, Ferra Yeni Rachmawati Yuli Priyana Yulianti, Reistu Tri Yumhi, Yumhi Yusnaldi, Yusnaldi Zaman, Badru Zebua, Agung Trysman