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All Journal Asian Journal of Community Services (AJCS) Jurnal Kedokteran Hewan The International Journal of Tropical Veterinary and Biomedical Research Buletin Pengabdian
Mulyadi Adam, Mulyadi
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Education Engineering Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary

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Journal : Jurnal Kedokteran Hewan

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STUDI HISTOKIMIA LEKTIN PADA SEL-SEL SPERMATOGENIK TESTIS MUNCAK (Muntiacus muntjak muntjak) (Lectin Histochemical Study of Testicular Spermatogenic Cells in Muntjak (Muntiacus muntjak muntjak)) Wahyuni, Sri; Agungpriyono, Srihadi; Adnyane, I. Ketut Mudite; Hamny, Hamny; Jalaluddin, Muhammad; Gholib, Gholib; Akmal, Muslim; Adam, Mulyadi; Aliza, Dwinna; Siregar, Tongku Nizwan
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i1.3396

Abstract

The objective of this study was to identify the type of specific glycoconjugates and its distribution in testicular spermatogenic cells in muntjak (Muntiacus muntjak muntjak) based on lectins histochemistry. An adult male muntjak aged 4-5 years old in hard antler period was used in this study. Testicular tissue was fixed in Bouin solution and processed histologically. Histochemistry method was performed using six types biotinylated lectins such as peanut agglutinin (PNA), soybean agglutinin (SBA), wheat germ agglutinin (WGA), ricinus communis agglutinin (RCA), concanavalin A (Con A), and ulex europaeus agglutinin I (UEA I) with 20 g/ml of concentration for PNA lectins and 15g/ml for other type of lectins. The results showed that glycoconjugates were detected by all type of lectins except UEA I in testicular spermatogenic cells with variation in distribution pattern and also the intensity of lectins binding. Glycoconjugates -galactose, -glucose, mannose, Nacetylgalactosamine, N-acetylglucosamine and sialic acid were stained intensely by lectins in golgy-cap phase and acrosomal phase of spermatids. Glycoconjugate N-acetylgalactosamine was the sugar residues which distributed abundantly that marked by positive reaction with PNA, SBA, and RCA lectins. In conclusion, glycoconjugates are detected in testicular spermatids cells of muntjak indicated that glycoconjugates have an important role in spermatogenesis particularly in spermiogenesis.Key words: glycoconjugates, lectins, spermatid, spermatozoa, muntjak
MEASUREMENT OF SERUM TESTOSTERONE IN KACANG GOAT BYUSING ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) TECHNIQUE: THE IMPORTANCE OF KIT VALIDATION (Pengukuran Testosteron Serum Kambing Kacang dengan Teknik Enzyme-Linked Immunosorbent Assay (ELISA): Pentingnya Validasi Kit) G, Gholib; Wahyuni, Sri; Kadar, Okta Hilda; Adam, Mulyadi; Lubis, Triva Murtina; A, Azhar; Akmal, Muslim; Siregar, Tongku Nizwan; Armansyah, Teuku; Nugraha, Taufiq Purna
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i1.3367

Abstract

This study was conducted to validate a commercial testosterone enzyme-linked immunosorbent assay (ELISA) kits (DRG EIA-1559) inanalytic and biological manner for measuring serum testosterone concentrations in kacang goats. This study used 18 healthy kacang goats, six bucks (2 years), six kids (6 months), and six does (2 years). Blood samples were collected from jugular vein and prepared as serum. Two validation tests were performed, an analytical validation comprises a parallelism, accuracy, precision and sensitivity and a biological validation by comparing testosterone concentration from bucks, kids, and does. Testosterone concentrations were measured using ELISA technique. Data of analytical validation were analyzed descriptively and test of equality of slope was performed to see the parallelism between samples and standard curves. Analysis of variance (ANOVA) was used for biological validation data. Results of parallelism showed that sample curve was parallel to the standard curve. Accuracy, precision (% CV of intra-and inter-assay) and sensitivity of the assay were 99.654.27%, 10%, 15% and 0.083 ng/ml, respectively. Results of biological validation showed that the assay used were accurately measured testosterone which testosterone concentrations in bucks were significantly higher compared to kids and does (P0.05). In conclusion, a commercial testosterone ELISA kits (DRG EIA-1559) is a reliable assay for measuring serum testosterone concentration in kacang goats. Key words: analytical and biological validations, ELISA, testosterone, kacang goat
PEMBERIAN EKSTRAK EPIDIDIMIS BERPOTENSI MENINGKATKAN KUALITAS SPERMATOZOA KAMBING JANTAN LOKAL Akmal, Muslim; Siregar, Tongku Nizwan; Wahyuni, Sri; Hambal, Muhammad; S, Sugito; A, Amiruddin; S, Syafruddin; R, Roslizawaty; Z, Zainuddin; Adam, Mulyadi; G, Gholib; Iskandar, Cut Dahlia; R, Rinidar; Asmilia, Nuzul; H, Hamny; J, Joharsyah; S, Suriadi
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i2.2839

Abstract

Penelitian ini bertujuan mengetahui pengaruh pemberian ekstrak epididimis (EE) terhadap peningkatan kualitas spermatozoa kambing jantan lokal. Dalam penelitian ini digunakan 12 ekor kambing jantan lokal, berumur 1,5 tahun dengan bobot badan 10-15 kg dan dibagi atas empat kelompok (K0, KP1, KP2, dan KP3). Kelompok K0, hanya diinjeksi dengan NaCl fisiologis sedangkan kelompok KP1, KP2, dan KP3 diinjeksi EE masing-masing 1, 2, dan 3 ml/ekor selama 13 hari berturut-turut. Pada hari ke-14, dilakukan pengambilan semen kambing dengan elektroejakulator dan selanjutnya dilakukan pemeriksaan kualitas spermatozoa. Hasil penelitian menunjukkan bahwa pemberian EE dengan dosis 1 dan 3 ml/ekor EE selama 13 hari berturut-turut menyebabkan peningkatan kualitas spermatozoa dibanding kelompok kontrol. Berdasarkan hal tersebut dapat disimpulkan bahwa EE berpotensi meningkatkan kualitas spermatozoa pada kambing jantan lokal.
INHIBIN B MENURUNKAN KONSENTRASI FOLLICLE STIMULATING HORMONE (FSH) PADA TIKUS PUTIH (Rattus norvegicus): UPAYA PENGEMBANGAN KONTRASEPSI HORMON PRIA BERBASIS PEPTIDA Akmal, Muslim; A, Aulanniam; Widodo, M. Aris; Sumitro, Sutiman B.; Purnomo, Basuki B.; Siregar, Tongku Nizwan; Hambal, Muhammad; A, Amiruddin; S, Syafruddin; Aliza, Dwinna; Sayuti, Arman; Adam, Mulyadi; Armansyah, T.; Rahmi, Erdiansyah
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i1.2788

Abstract

Penelitian ini bertujuan mengetahui efek injeksi inhibin B terhadap penurunan konsentrasi follicle stimulating hormone (FSH) di dalamserum pada tikus putih (Rattus norvegicus). Dalam penelitian ini digunakan 24 ekor tikus putih berjenis kelamin jantan dengan strain Wistar berumur 4 bulan dengan bobot badan 150-200 g. Tikus-tikus dikelompokkan secara acak ke dalam 4 kelompok, yaitu KK0, KP1, KP2, dan KP3, masing-masing kelompok terdiri atas 6 ekor. Kelompok KK0 merupakan kelompok kontrol hanya diinjeksi dengan phosphate buffer saline (PBS), sedangkan kelompok KP1, KP2, dan KP3 diinjeksi dengan inhibin B dengan dosis berturut-turut 25, 50, dan 100 pg/ekor. Injeksi inhibin B dilakukan secara intraperitoneum sebanyak 5 kali selama 48 hari dengan interval waktu 12 hari. Injeksi pertama inhibin B dilarutkan dengan0,05 ml PBS dan 0,05 ml Freuds complete adjuvant (FCA). Injeksi kedua sampai kelima, inhibin B dilarutkan dengan 0,05 ml PBS dan 0,05 ml Freuds incomplete adjuvant (FICA). Pada hari ke-6 setelah injeksi inhibin B terakhir, tikus dikorbankan secara dislocatio cervicalis,lalu darah dikoleksi langsung dari jantung dan didiamkan hingga didapatkan serum untuk pemeriksaan konsentrasi FSH dengan menggunakan metode enzyme-linked immunosorbent assay (ELISA). Hasil penelitian menunjukkan bahwa injeksi inhibin B dengan dosis 100 pg/ekor menurunkan konsentrasi FSH secara nyata (P0,05) bila dibandingkan dengan kelompok kontrol. Berdasarkan hal tersebut, inhibin B berpeluang untuk dikembangkan sebagai kandidat kontrasepsi pria hormon berbasis peptida.
Co-Authors A Sakir, A Sakir A, Aulanniam Abdullah Hamzah, Abdullah Afifuddin Afifuddin, Afifuddin Amalia Sutriana Amiruddin Amiruddin Arifin, Tazul Arman Sayuti Aulia, Usma Azhar A, Azhar Basuki B. Purnomo budianto panjaitan Cut Dahlia Iskandar Cut Nila Thasmi, Cut Nila Darwanis Darwanis Dasrul Dasrul Dwinna Aliza Erdiansyah Rahmi Fadhil, Rahmat Fadillah, Nurul Aini Faisal Faisal Faisal Mustafa Fajar Fakri Fajri, Fauzan Fathurrahman, Fadhil Fitrah Khairi Gani, Fadli A Gani, Fadli A. Gholib G, Gholib Gholib Gholib, Gholib Hafizuddin Hafizuddin Hafizuddin, H Halim, Hendra Hamny H, Hamny Hamny Hamny Hamny Sofyan I. Ketut Mudite Adnyane, I. Ketut Mudite Joharsyah J, Joharsyah Juli Melia Kesuma, T. Meldi Ketut Adnyane Mudite M. Aris Widodo M. Shabri Abd. Majid Mahdi Abrar Masda Admi Mayuri, Herdina Muhammad Bahi Muhammad Hambal Muhammad Hasan Muhammad Isa Muhammad Jalaluddin, Muhammad Muslim Akmal N. Nazaruddin Nanda Yulian Syah Nellita Meutia, Nellita Nirmala, Fadya Nora Usrina Nuzul Asmilia Okta Hilda Kadar, Okta Hilda R Roslizawaty, R Ramadhana, Cut Erika Razali Daud Ridwan Nurdin Rinidar R, Rinidar Riski Ananda, Riski Ritonga, Mudhita Zikkrullah Roslizawaty r Roslizawaty Roslizawaty, Roslizawaty Rosmaidar Rosmaidar, Rosmaidar Sari, Meutia Dwi Novita Siregar, M. Ridha Sitepu, Novi Indriyani Sri Wahyuni Srihadi Agungpriyono Sugito Sugito Suriadi S, Suriadi Suryani, Elfi Satria Sutiman B. Sumitro Syafruddin s Syafruddin Syafruddin Syafruddin, S Syahrizal, Teuku Muhammad T. Armansyah Taufan Hidayat Taufiq Purna Nugraha, Taufiq Purna Tongku Nizwan Siregar Triva Murtina Lubis Umuri, Khairil Zainuddin Z Zainuddin, Zainuddin Zamzami, Rumi Sahara Zulfitri Zulfitri, Zulfitri Zulkifli, Baidillah