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All Journal EKSAKTA: Journal of Sciences and Data Analysis Science and Technology Indonesia Dharma Bakti
Habibi Hidayat, Habibi
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Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Education Environmental Science Materials Science & Nanotechnology Physics Social Sciences Other

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Journal : EKSAKTA: Journal of Sciences and Data Analysis

 1 
Chemical Compound Identification of Lactic Acid Bacteria (LAB) From Porang Tubers Waste (A. muelleri blume) as Probiotic Hidayat, Habibi; Nakita Heniar Septyana; Riang Adeko; Dinda Rizki Febriani; Amri Setyawati1
EKSAKTA: Journal of Sciences and Data Analysis VOLUME 5, ISSUE 2, October 2024
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/EKSAKTA.vol5.iss2.art5

Abstract

The production of amylase produced by lactic acid bacteria (LAB) has better stability. The purpose of this study was to utilize and determine the characteristics of LAB isolates from porang skin waste which have the potential as probiotics and the activity of amylase enzymes. Isolation was done by fermentation method for 48 h with the addition of starter Pediococcus acidilactici that had been cultured. The results showed that the number of colonies of N1 isolates was 49 ×108 CFU/mL with regular small round colonies, having a convex elevation, the edges of the colonies were round, yellowish white, and Gram positive bacteria because they produced a purple color on Gram staining. Acid resistance test at pH 2.0; 3.0; 4.0; 5.0 and 6.0, the isolates N1 was able to survive at each pH because it showed turbidity in the growth medium. The antimicrobial activity test showed a strong zone of inhibition on S. aureus and S. pyogenes of 10,33 and 11,17 mm and a moderate zone of inhibition on K. pneumoniae, S. dysentriae, and P. aeruginosa, respectively, which was 6,33; 7,7 and 7,5 mm. The positive N1 isolate contained alkaloids as bioactive compounds from the results of phytochemical screening and the results of the FTIR analysis showed that specific groups of alkaloids were N-H, C-N, and C=O at wave numbers 3423.30 cm-1; 1235.45 cm-1; and 1634.81 cm-1.
Biochemical Test and Antimicrobial Potentials of Bandotan (Ageratum conyzoides) Leaves against Pathogenic bacteria in the Water Riang Adeko; Hidayat, Habibi; Andriana Marwanto; Sri Mulyati; Wiwit Aditama
EKSAKTA: Journal of Sciences and Data Analysis VOLUME 5, ISSUE 2, October 2024
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/EKSAKTA.vol5.iss2.art10

Abstract

Water cleaning technology using coagulation has already been carried out use a plants in the coagulation process has never been done before, especially using plants that have potential to be a source of natural coagulants is Bandotan (Ageratum conyzoides). Plants that have the potential to be a source of natural coagulants are plants that contain a lot of protein. Each type of protein has an isoelectric point at a different pH like isoelectric point, the protein will have a neutral charge. . The calculation results of the number of colonies in samples were B1, B2, and B3 with dilutions of 266. 105, 9. 107 and 104. 109 CFU/mL. The results of Gram staining identification showed that the B1, B2 and B3 isolates had the shape are basil, basil and coccus (round) with Gram positive. The bacteria thrive in different habitats, so bacteria from the sample have been very cloudy to pH of 6.0 and 8.0 because neutralophiles properties. From the B3 isolate sample have high clear zone for Gram positive (S. aureus) of 13 mm more than Gram negative ( E. coli) of 11 mm. So that, the bandotan is able to inhibit the growth of pathogenic bacteria in the water
Co-Authors Adeko, Riang Amri Setyawati1 Andriana Marwanto Beta Wulan Febriana Daud, Muhammad Fauzi Dinda Rizki Febriani Emilia Fajarwati, Febi Indah Fauziyyah, Anas Zahra Haneef, Mehru Nisha Muhamad Ika Yanti, Ika Is Fatimah Kamari, Azlan Koda, M. Idris Krisna Merdekawati, Krisna Nakita Heniar Septyana Nurlaela, Nunung Oh, Won-Chun Sagadevan, Suresh Sri Mulyati Widinda Normalia Arlianty, Widinda Normalia Wiwit Aditama Wiyantoko, Bayu