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All Journal CALYPTRA : Jurnal Ilmiah Mahasiswa Universitas Surabaya Jurnal Farmasi Klinik Indonesia MPI (Media Pharmaceutica Indonesiana) Molecular and Cellular Biomedical Sciences (MCBS) Syntax Literate: Jurnal Ilmiah Indonesia Jurnal Ilmiah Kesehatan Rustida Keluwih: Jurnal Sains dan Teknologi Pharmaceutical Sciences and Research (PSR)
Mariana Wahjudi, Mariana
Universitas Surabaya
Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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Journal : MPI (Media Pharmaceutica Indonesiana)

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Profil Resistensi Isolat Klebsiella pneumoniae, Haemophilus sp. dan Streptococcus viridans dari Sputum Pasien ISPA terhadap Amoksisilin Raharjo, Dian Natasya; Islamie, Ridho; Wahjudi, Mariana; Apriyani, Dhea Orinta; Wartini, Luh Risma; Dewi, Ni Putu Nila Sulistia
MPI (Media Pharmaceutica Indonesiana) Vol. 6 No. 1 (2024): JUNE
Publisher : Fakultas Farmasi, Universitas Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24123/mpi.v6i1.6523

Abstract

Infeksi Saluran Pernapasan Akut (ISPA) merupakan radang akut yang disebabkan oleh infeksi mikroorganisme dengan angka kejadian cukup tinggi di Indonesia. Sebanyak total 49 bakteri yang terdiri dari 13 isolat Klebsiella pneumoniae, 18 isolat Haemophilus sp., dan 18 isolat Streptococcus viridans koleksi laboratorium setempat diperoleh dari sputum pasien ISPA di berbagai puskesmas di Kota Surabaya selama bulan Desember 2022. Penelitian ini bertujuan untuk mengetahui resistensi dari 49 koleksi isolat tersebut terhadap amoksisilin. Pengujian dilakukan untuk memperoleh MIC (Minimum Inhibitory Concentration) amoksisilin menggunakan metode agar dilution pada media Mueller Hinton Agar. Nilai MIC kemudian dibandingkan dengan MIC breakpoints pada Clinical and Laboratory Standards Institute (CLSI) dan European Committee on Antibiotic Susceptibility Testing (EUCAST). Hasil penelitian menunjukkan 61,54% (8/13) isolat Klebsiella pneumoniae, 100% (18/18) isolat Haemophilus sp., dan 11,11% (2/18) isolat Streptococcus viridans resisten terhadap amoksisilin. Oleh karena itu dapat disimpulkan perlunya rekomendasi antibiotik alternatif sebagai pengganti amoksisilin untuk pengobatan ISPA. Acute Respiratory Infection (ARI) is an acute inflammation caused by microorganism infection with a high incidence in Indonesia. The local microbiology laboratory had successfully collected 49 bacterial isolates from the ARI patient's sputum in various Public Health Centers in Surabaya, Indonesia during December 2022. The isolates consisted of 13 isolates of Klebsiella pneumoniae, 18 isolates of Haemophilus sp., and 18 isolates of Streptococcus viridans. This study aimed to determine the resistance of the 49 isolates to amoxicillin. Testing was carried out to obtain the MIC (Minimum Inhibitory Concentration) of amoxicillin using the agar dilution method on Mueller Hinton Agar media. The MIC value was then compared with the MIC breakpoints at the Clinical and Laboratory Standards Institute (CLSI) and the European Committee on Antibiotic Susceptibility Testing (EUCAST). The results showed that 61.54% (8/13) of Klebsiella pneumoniae isolates, 100% (18/18) of Haemophilus sp. isolates, and 11.11% (2/18) of Streptococcus viridans isolates were resistant to amoxicillin. Therefore, it can be concluded that it is necessary to recommend alternative antibiotics as a substitute for amoxicillin for the treatment of ARI.
Air Daun Sirih (Piper betle L.) Tidak Berpotensi Memicu Resistensi Sel Escherichia coli pada Dosis Pemakaian Secara Traditional Castoeri, Yeslia Naomi; Suryadjaya, Ernest; Wahjudi, Mariana
MPI (Media Pharmaceutica Indonesiana) Vol. 4 No. 1 (2022): JUNE
Publisher : Fakultas Farmasi, Universitas Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24123/mpi.v4i1.4706

Abstract

Air rebusan daun sirih (Piper betle L.), disebut juga air sirih, telah lama digunakan masyarakat di Indonesia. Pemakaian air sirih secara terus-menerus menimbulkan kekhawatiran pada muculnya bakteri yang resisten, seperti pada kasus paparan bakteri dengan minyak pinus. Penelitian ini dilakukan untuk mengetahui potensi terjadinya perubahan kepekaan sel Escherichia coli setelah dipaparkan air sirih dengan kadar yang umum digunakan oleh masyarakat (5% b/v). Metode yang digunakan adalah dengan pemaparan sel E. coli dengan air sirih 5% b/v, mulai dari generasi 0 hingga generasi ke-130. Uji konfirmasi ada tidaknya perubahan kepekaan sel terhadap air sirih dilakukan dengan penentuan kadar hambat minimum dan hambatan pertumbuhan sel dengan metode difusi agar. Hasil penelitian menunjukkan bahwa sel pada generasi ke-0, 10, 40, 110, dan 130 tidak terhambat pertumbuhannya oleh air sirih walaupun densitas sel setelah terpapar air sirih dari generasi ke-0 hingga ke-130 cenderung mengalami penurunan. Zona hambatan pertumbuhan sel dari semua generasi terhadap antibiotik imipenem, ceftazidime, ciprofloxacin dan ampicilin juga tidak berbeda signifikan. Berdasarkan hasil penelitian dapat disimpulkan bahwa sel E. coli yang terpapar air sirih pada kadar pemakaian masyarakat tidak berubah kepekaannya terhadap air sirih dan beberapa antibiotika uji.
Secondary Metabolites of Various Indonesian Medicinal Plants as SARS-CoV-2 Inhibitors: In Silico Study Tungary, Emilia; Wahjudi, Mariana; Kok, Tjie
MPI (Media Pharmaceutica Indonesiana) Vol. 4 No. 2 (2022): DECEMBER
Publisher : Fakultas Farmasi, Universitas Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24123/mpi.v4i2.5255

Abstract

Corona virus disease 2019 caused by SARS-CoV-2 infection emerged in late 2019 and still become a worldwide pandemic up to this point with the drug remain unavailable. Meanwhile, Indonesia has an abundance variety of medicinal plants that are potential to be developed as inhibitors. By using the key role proteins as drug targets, namely spike glycoprotein and RNA-dependent RNA polymerase (RdRp) of delta variant of SARS-CoV-2 (which is known as strongly transmitted and highly virulent), we can develop inhibitors for the target proteins from potential Indonesian medicinal plants to prevent the protein interactions for viral entry and proliferation that leading to organ disfunction and death. This study aimed to identify the secondary metabolites of various Indonesian medicinal plants as SARS-CoV-2 inhibitors. The 184 ligands from nine plants were collected from IJAH webserver and their SMILES notation were collected from PubChem. Meanwhile 3D structures of spike glycoprotein (PDB ID: 6VXX) and RdRp (PDB ID: 6M71) were obtained from protein data bank (PDB). Molecular docking was conducted between ligands and the two SARS-CoV-2 proteins using Autodock Vina in PyRx with hesperidin and remdesivir as control compounds. Several potential compounds were selected for drug-likeness analysis and toxicity analysis. Results showed that lantanolic acid has the same amino acid interaction with RdRp as the control compound. It formed a hydrogen bond with Ser784 and hydrophobic bonds with Tyr32 and Ser7709. It had lower binding affinity than the control compounds, eligible as oral drug, and had LD50 of 2589 mg/kg.
Co-Authors Amelia Lorensia Andayani, Tri M. Antonius, Yulanda Apriyani, Dhea Orinta Castoeri, Yeslia Naomi Dewi, Ni Putu Nila Sulistia Doddy de Queljoe, Doddy de Erdiansyah, Muhammad Islamie, Ridho Iwansyah, Assidiq Zidane Maranatha, Daniel Marisca Evalina Gondokesumo, Marisca Evalina Meira, Gracelynn Novandrie Zakharia Daud Novandrie Zakharia Daud, Novandrie Zakharia Nurrosyidah, Iif Hanifa Prasetyaningtyas, Herawati Dwi Raharjo, Dian Natasya Saksono, Astrella Amanda Putri Santoso, Hadinata Simanjuntak, Inana Ratu Soripada Suryadinata, Rivan V. Suryadjaya, Ernest Tungary, Emilia Wartini, Luh Risma Wonggo, Dhammiko