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All Journal Jurnal Teknologi Dan Industri Pangan Jurnal Biotek Medisiana Indonesia Medical Journal of Indonesia Majalah Kedokteran Journal of Medicine and Health Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education EKOTONIA: Jurnal Penelitian Biologi, Botani, Zoologi dan Mikrobiologi Cakradonya Dental Journal Journal of Clinical Microbiology and Infectious Diseases Journal of Medicine and Health
Mardiastuti H Wahid
Departemen Mikrobiologi Klinik, Fakultas Kedokteran, Universitas Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Neuroscience Public Health

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Journal : Jurnal Teknologi Dan Industri Pangan

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Lactobacillus plantarum PADA FESES INDIVIDU DEWASA SEHAT YANG MENGONSUMSI Lactobacillus plantarum IS-10506 DARI DADIH [Lactobacillus plantarum in Stool of Apparently Healthy Adults Consuming Lactobacillus plantarum IS-10506 from Dadih] Azmier Adib; Mardiastuti H Wahid; Pratiwi Sudarmono; Ingrid S. Surono
Jurnal Teknologi dan Industri Pangan Vol. 24 No. 2 (2013): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (539.775 KB) | DOI: 10.6066/jtip.2013.24.2.154

Abstract

A placebo double blind pre-post human study was conducted in apparently healthy adults. There were two treatment groups consisting of Group A and B representing probiotic and placebo group, respectively. Twenty four participants were randomly assigned, each supplemented with either placebo or probiotic Lactobacillus plantarum IS-10506. The micro encapsulated powder was given at a dose of 2.6x1010 CFU/day for 21 consecutive days. Stool samples were collected before and after the supplementation. The fresh stool samples were analyzed for the viability of Lactobacillus sp. by conventional plate count method in MRS agar. Some stool samples were kept frozen to be analyzed by using real time PCR to trace back the availability of Lactobacillus plantarum with species specific primer. The Lactobacillus sp. in stools of healthy adults given microencapsulated probiotic Lactobacillus plantarum IS-10506 powder was significantly more than those who consumed microencapsulated placebo powder. Molecular detection by qPCR confirmed the availability of Lactobacillus plantarum in fecal samples of the probiotic group after given the supplementation for 21 days. The molecular detection validation confirmed that probiotic Lactobacillus plantarum was available in the fecal samples of the probiotic group of healthy adults. However, the availability and viability of Lactobacillus plantarum were not consistently found in the intestinal tract.
Co-Authors Akbar, Resti Rahmadika Andi Yasmon Angga W Lokeswara, Angga W Ariyani Kiranasari Augustine Natasha Aziz, Mochamad Helmi Azmier Adib Azmier Adib Chairinda Dachwan Conny Tjampakasari Effendi, Evita Erie Yuwita Sari Fathan, Hafizah Fera Ibrahim Ibnu Agus Ariyanto Ika Ningsih Ika Ningsih Indah S. Widyahening Ingrid S. Surono Luthfi Saiful Arif Mochamad Helmi Aziz Mursinah Mursinah Mursinah, Mursinah Ni Made Adi Tarini Nurul Husna Oliver E Yausep, Oliver E Pratiwi Pujilestari Sudarmono Raksheeth Agarwal, Raksheeth Retno Asti Werdhani Retno Kadarsih Rimonta F Gunanegara Rimonta F Gunanegara, Rimonta F Rivia Gina Rahmawaty Samsuridjal Djauzi Sari, Meutia Kumala Sharon Hanmy Angel, Sharon Hanmy Sitohang, Irma Bernadette Simbolon Yeva Rosana