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All Journal Ovozoa: Journal of Animal Reproduction
Budi Utomo
Division of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Indonesia
Agriculture, Biological Sciences & Forestry Veterinary

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The effect of addition of melon (Cucumis melo L.) flesh juice into Lactated Ringer’s-egg yolk extender on spermatozoa plasma membrane integrity and spermatozoa morphological abnormalities of semen of native rooster Dinda Galuh Pitaloka; Mas’ud Hariadi; Suherni Susilowati; Budi Utomo; Tatik Hernawati; Eka Pramyrtha Hestianah; Samuel Inioluwa Akeju
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 1 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i1.2023.49-58

Abstract

This study aims to determine the effect of adding melon (Cucumis melo L.) juice to Lactated Ringer’s-egg yolk extender on the intact plasma membrane (IPM) and morphological abnormalities of native rooster spermatozoa. Five ejaculate each from two native roosters (Gallus gallus domesticus) were divided into four equal volumes. Ejaculate was extended 1:10 (v/v) in Lactated Ringer's-egg yolk without or with the addition of of 2, 4, and 8% (v/v) melon flesh juice, respectively for groups T0, T1, T2, and T3. Extended semen was stored in a refrigerator (5°C) and evaluated every two hours. The results showed that IPM of all groups decreased (p <0.05) consistently, lasting more than 40% for six hours in the T0 and T1 groups and four hours in the T2 and T3 groups. The percentage of spermatozoa morphological abnormalities in all groups stored for six hours was higher (p <0.05) than before storage. However, it was not significantly different (p >0.05) from that which had been stored for two and four hours. It was concluded that native rooster semen extended in Lactated Ringer's-egg yolk without (group T0) or with 2% melon flesh juice (group T1) and stored at 5°C retained spermatozoa plasma membrane integrity of more than 40% and morphological abnormalities of less than 20% for six hours. While the addition of 4 % (T2) and 8% (T3) melon flesh juice maintained the percentage of IPM and spermatozoa morphological abnormalities for only four hours.
Impact of green tea (Camellia sinensis) leaf extract in skim milk-goose egg yolk semen extender on the quality of Sapudi ram spermatozoa stored at 5°C Ardina Sahra Miranda; Tri Wahyu Suprayogi; Budi Utomo; Suherni Susilowati; Yeni Dhamayanti
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 3 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i3.2023.148-156

Abstract

Livestock production requires Sapudi rams, a breed native to Indonesia, to meet meat demand and food security. In artificial high-quality frozen semen is needed to spread Sapudi rams. To maximize the survival of spermatozoa during cryopreservation, semen should be stored in an extender. Green tea leaf extract (GTLE) and skim milk-goat egg yolk (SM-GEY) may be a good cryoprotectants due to their antioxidant properties. This study aimed to determine the effect of adding GTLE to the SM-GEY extender on the quality of Sapudi ram spermatozoa stored at 5°C. The fresh semen sample was divided into four different GTLE treatment groups, which each contained a 0.1 mL semen sample and a 25-mL extender of SM-GEY. Group T0: no GTLE added to SM-GEY; Groups T1, T2, and T3: 0.1 mL semen diluted in 25 mL SM-GEY with 0.05, 0.10, and 0.15 mg GTLE. Extended semen was then stored at 5°C, and its quality was evaluated daily for five days. The variables observed included spermatozoa motility, viability, and membrane integrity. Data were analyzed using one-way analysis of variance followed by Duncan's test using Statistical Program and Service Solution version 23. The result of this study was that adding 0.05 mg GTLE to 25 mL of SM-GEY extender significantly maintained the spermatozoa motility, viability, and plasma membrane integrity of Sapudi ram spermatozoa for three days at 5°C (p <0.05). Therefore, it could be concluded that adding 0.05 mg of GTLE to the SM-GEY extender preserved Sapudi ram spermatozoa's motility, viability, and membrane integrity for three days at 5°C.
Effect of white guava (Psidium guajava L.) fruit juice on the quality of lead acetate induced rats (Rattus norvegicus) spermatozoa Annisa Alifia; Sri Mulyati; Wurlina Wurlina; Tatik Hernawati; Budi Utomo; Soeharsono Soeharsono; Sri Agus Sudjarwo; Muhammad Thohawi Elziyad Purnama
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 3 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i3.2023.131-140

Abstract

This study aims to determine the effect of the administration of white guava (Psidium guajava L.) fruit juice on spermatozoa plasma membrane integrity (PMI), morphological abnormality, viability, and motility of lead acetate induced rats (Rattus norvegicus). Twenty-five male rats were divided into five groups: NC (negative control) group, rats were administered with distilled water twice daily at four-hour intervals; T0 (positive control) group, rats were administered daily with lead acetate 50 mg/kg bw and distilled water four hours later; T1, T2, and T3 groups, rats were administered daily with lead acetate 50 mg/kg bw and 0.5 mL of 25, 50, and 100% white guava fruit juice four hours later. The treatment of the rats was conducted for 14 days, and on day 15, all rats were sacrificed to assess the spermatozoa quality. Data was analyzed using ANOVA followed by Duncan's multiple range test at a confidence level of 95%. The results showed that exposure to lead acetate (T0) caused lower spermatozoa PMI, viability, and motility as well as higher spermatozoa morphological abnormalities (p <0.05) compared to those of the T0 group. Administration of white guava fruit juice starting at a dose of 25% (T1) resulted in higher spermatozoa motility, viability, and PMI as well as lower spermatozoa morphological abnormalities (p <0.05) compared to rats in the T0 group. It could be concluded that white guava fruit juice maintained the spermatozoa quality of lead acetate induced rats.
Reproduction record of captive Sumatera elephant (Elephas maximus sumatranus) at Way Kambas National Park, Indonesia Keren Jayanti Sofie Adi; Budiarto Budiarto; Hermin Ratnani; Pudji Srianto; Budi Utomo; Suzanita Utama; Setiawan Koesdarto; Nurhusien Yimer
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 3 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i3.2023.157-165

Abstract

This research aims to determine reproductive data on Sumatran elephants (Elephas maximus sumatranus) in the ETC and ERU of Way Kambas National Park, Indonesia during 1988-2021. Data recorded from the elephant population at both locations (ETC and ERU) includes the number, gender of elephants, elephant calves, ages and birth dates. Calving intervals and service periods were calculated from calving records of cows with a minimum parity of two. Data collection produced data on 47 elephant calves from 13 female elephants with at least twice the parity and 12 primiparous cows. Elephant reproductive records at ETC and ERU were: age (37.44 ± 9.03 vs. 29.75 ± 3.30 years), parity (2.78 ± 1.09 vs. 2.50 ± 0.58), and age at first birth (18, 11 ± 3.92 vs. 17.75 ± 0.50 years). While the calving interval was 1857.56 ± 870.81 vs. 1833.00 ± 305.18 days, and service period respectively 1229.44 ± 846.18 vs. 1210.50 ± 283.59 days, respectively. It can be concluded that the calving interval and service period for captive elephants at ETC and ERU were not much different and were within the normal range. Young cows showed better reproductive efficiency than older cows. Monitoring calves is very important. Further assistance is needed to improve elephant mobile veterinary services, increase diagnostic laboratory capacity, and educate camp managers, veterinary assistants, and mahouts about elephant diseases, their monitoring and treatment. In addition, the use of reproductive technology such as monitoring ovulation using ultrasound and carrying out artificial insemination was expected to increase reproductive efficiency.
Co-Authors Annisa Alifia Ardina Sahra Miranda Budiarto Budiarto Dinda Galuh Pitaloka Eka Pramyrtha Hestianah Hermin Ratnani Keren Jayanti Sofie Adi Mas’ud Hariadi Muhammad Thohawi Elziyad Purnama Nurhusien Yimer Pudji Srianto Samuel Inioluwa Akeju Setiawan Koesdarto Soeharsono Soeharsono Sri Agus Sudjarwo Sri Mulyati Suherni Susilowati Suzanita Utama Tatik Hernawati Tri Wahyu Suprayogi Wurlina, W Yeni Dhamayanti