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All Journal e-Journal Pustaka Kesehatan STOMATOGNATIC- Jurnal Kedokteran Gigi Insisiva Dental Journal : Majalah Kedokteran Gigi Insisiva Dental Journal (Majalah Kedokteran Gigi) Jurnal Kedokteran Gigi Universitas Padjadjaran Health Notions The Indonesian Journal of Health Science ARTERI : Jurnal Ilmu Kesehatan
Sri Lestari
Fakultas Kedokteran Gigi Universitas Jember
Dentistry Electrical & Electronics Engineering Health Professions Library & Information Science Medicine & Pharmacology Neuroscience Nursing Public Health

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Journal : Dental Journal (Majalah Kedokteran Gigi)

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The effect of exposure duration of self etch dentin bonding on the toxicity of human gingival fibroblast of cell culture Sri Lestari
Dental Journal (Majalah Kedokteran Gigi) Vol. 41 No. 2 (2008): June 2008
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.611 KB) | DOI: 10.20473/j.djmkg.v41.i2.p91-94

Abstract

Self etch dentin bonding created to make light easily activate the application of composite resin on tooth surface. The monomer content has acid effect that could irritate tooth pulp. The purpose of this study was to evaluate the effect of light exposure duration of self etch dentin bonding on toxicity of human gingival fibroblast of cell culture by MTT assay. Self etch dentin bonding was used as on experimental unit and the sample was exposed by visible light curing in different duration: 10, 20, 30 seconds and immerged in artificial saliva in pH 7 for 24 hours. 100 µl artificial saliva was exposed to human gingival fibroblast of cell culture 20.000 cells/100 µl RPMI for 24 hours. Toxicity was evaluated by MTT assay, optical density was measured using 550 nm spectrophotometer. The data was analyzed using Kruskal Wallis in 5% degree of significance. The result showed that increasing exposure duration (10, 20, 30 seconds) of self etch dentin bonding will reduce the toxicity of human gingival fibroblast of cell culture. It is concluded that 30 seconds-exposure of self etch dentin bonding will reduce the toxicity of human gingival fibroblast of cell culture.
Co-Authors Annisa K Disya Dwi Maulidiyah Dwi Merry Christmarini Robin Dwi Warna Aju F Dwi Warna Aju Fatmawati Dyah Setyorini Ekiyantini Widyowati Farah Firdha Abadhia Hafida Mariyatin Hestieyonini Hadnyanawati Hidayatul F. I Dewa Ayu Ratna Dewanti I, Istibsyaroh Ilonavia Satiti Istibsyaroh I Melok Aris Wahyukundari Narita Ajeng Loviana Nurbaetty Rochmah Pudji Astuti Qhorie Azra Bintang Raditya Nugroho Ratih Delio Rakhmadian Ristya Widi Endah Yani Rochmah, Nurbaetty Supriyadi Supriyadi