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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Indonesian Journal of Agricultural Science Indonesian Journal of Biotechnology Jurnal Penelitian Pertanian Tanaman Pangan
Suharsono, Suharsono
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor (Bogor Agricultural University), Kampus IPB Darmaga, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Immunology & microbiology Materials Science & Nanotechnology

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Journal : Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)

 1 
Introgresi Gen CsNitr1-L dari Transgenik Nipponbare ke Ciherang dan Analisis Pewarisannya pada Generasi BC3F4 ,, Nazarudin; ,, Suharsono; ,, Sustiprijatno
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 42 No. 3 (2014): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (693.095 KB) | DOI: 10.24831/jai.v42i3.9160

Abstract

ABSTRACTCsNitr1-L gene is a gene encoding nitrites transporter and is included in the group of proton oligopeptide transporter (POT) gene family. The absorption of nitrites by plants expressing this transporter becomes efficient. The gene encoding this protein (CsNitr1-L) under the control of 35S CaMV Promoter had been introduced into rice plants (Oryza sativa L.) subspecies japonica cv. Nipponbare to transfer this gene. The japonica transgenic rice had been crossed with Ciherang variety followed by back-cross and self polination until BC3F4 generation. The aim of this study was to analyse introgression of CsNitr1-L gene in the transgenic rice BC3F4 generation. The transgenic rice plants in BC3F4 generation were selected based on the resistance to hygromicin. More than 90% population of BC3F4 are putative introgression rice lines carriying the transgene. The introgression of the transgene were indirectly confirmed by PCR analaysis using primer corresponding to hpt gene. The yield of introgression line was higher than these of original Ciherang cultivar. Four introgression lines (G3, G7, G8 and G11) that had higher yield were analysed by PCR. Result of the  analysis showed that these four transgenic plants carried the introgression.Keywords: proton oligopeptide transporter, nitrites transporter, efficient nitrogen, transgenic rice
Hubungan Metilasi DNA dengan Ekspresi Gen MADS-box pada Buah Mantel Tanaman Kelapa Sawit (Elaeis guineensis Jacq.) Anischan, Maharani; ,, Suharsono; Mathius, Nurita Toruan-; Kusnandar, Andree Sunanjaya
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 42 No. 3 (2014): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (488.105 KB) | DOI: 10.24831/jai.v42i3.9175

Abstract

ABSTRACT The presence of mantled fruit on large-scale clonal production of oil palm had trully decreased the oil productivity. Mantled phenotype is likely to be that of an epigenetic change involving DNA methylation and the MADS-box transcription factor gene which encoded floral organ homeotic transformation. The objectives of this research were to quantify the degree of methylation which determined fruit abnormality through Ultra Performance Liquid Chromatography (UPLC) and to study its correlation with the MADS-box EgAGL6, EgAG2, and EgAGA genes expressed on mantled fruit derived from oil palm clonal plants which have been quantified using Quantitative Real-Time PCR (qPCR). This research was arranged in two replications for each gene and cDNA. The expression of the target genes were compared to EF1-α1 as the reference gene. Through the Least Significant Difference (LSD) Test at 95% confidence level of qPCR result, EgAGL6 expression was significantly lower in mantled fruit which decreased from 1.88 fold in Abn m to 0.46 fold in Abn. EgAG2 expression was increased non-significantly from 0.91 fold in Abn m to 1.13 fold in Abn, while EgAGA expression was higher in mantled which increased significantly from 1.48 fold in Abn m to 1.71 fold  in Abn. Nuclease S1 digestion and UPLC revealed the genome-wide increase in DNA methylation on mantled fruit (18.33-19.55%) compared to its normal counterparts (5.67%). This increased in global DNA methylation was showed by the significant decreased in EgAGL6 transcript level of mantled fruit. This gene assumed to be involved in the development of mantled fruit. Keywords: DNA-methylation, MADS-box genes, Mantled fruit, Quantitative Real-Time PCR
Co-Authors Andree Sunanjaya Kusnandar Apon Zaenal Mustopa Aris Tjahjoleksono Bugi Ratno Budiarto Fatimah Fatimah Hasim Danuri Kusdianawati Maharani Anischan Nazarudin , Nugroho, K. Nurita Toruan- Mathius Pardal, Saptowo J. Pasmawati Pasmawati Rahayu, V. R. Sustiprijatno ,