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All Journal Biology, Medicine, & Natural Product Chemistry Majalah Obat Tradisional Meditory : The Journal of Medical Laboratory SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Jurnal Kreativitas PKM Jurnal Ilmu Kedokteran dan Kesehatan PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Medika Kartika : Jurnal Kedokteran dan Kesehatan Journal of Experimental and Clinical Pharmacy (JECP) Kontribusi: Jurnal Penelitian dan Pengabdian Kepada Masyarakat
Hanasia, Hanasia
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Control & Systems Engineering Dentistry Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Journal : Biology, Medicine,

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Presence of Salmonella sp. In Tilapia and Catfish from Cages in Pahandut Seberang Along the Kahayan River Flow Kurniawan, Mutiara; Praja, Rian Ka; Widiarti, Astri; Hanasia, Hanasia; Toemon, Angeline Novia
Biology, Medicine, & Natural Product Chemistry Vol 14, No 1 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.141.185-191

Abstract

Salmonellosis is a zoonosis, which means that it may be spread from animals to people through fish meat. It is a sickness brought on by an infection with the salmonella bacteria that infect humans and animals and invade the digestive tract. According to data from the Institute for Health Metrics and Evaluation, the global rate of salmonellosis in 2019 was 4.22 cases per 100,000 people, with a 1.72 fatality rate. Indonesia had a mortality rate of 2.42 and 5.82 per 100,000 people 2019. The objective of this study is to identify Salmonella sp. bacteria in 14 samples of river water, catfish (Clariidae), and tilapia (Oreochromis niloticus) raised in rivers. This study includes two water samples, three catfish samples, and nine tilapia samples from the cage of the Pahandut Seberang River in Palangka Raya City. It employs a descriptive research design using an experimental technique. Among the metrics noted is the detection of Salmonella sp. The study found that all water samples, tilapia, and catfish tested positive for Salmonella sp bacteria in the selective media Salmonella Shigella Agar (SSA), that 14 samples (100%) in the gram staining test displayed the traits of Salmonella sp bacteria, and that 5 samples (B N1, C N2-3 samples in tilapia, B L1 samples in catfish, and water sample 2 showed the type of Salmonella paratyphoid b bacteria) and 9 samples, namely samples Salmonella tyhpimarium bacteria were found in samples A L1, C L1 in catfish, B N2-3, C N1 in tilapia, and water 1. As a result, every sample surpassed the SNI 7388:2009 maximum level of microbial contamination, which is positive/25g. According to the Indonesian National Standardization Agency's SNI 7388:2009 guidelines for the upper limit of microbial contamination in food, the tilapia meat, catfish, and river water in the river cage of Kahayan Pahandut Seberang Palangka Raya City do not satisfy the requirements.
siRNAs targeting icaD Gene of Staphylococcus aureus to Inhibit Biofilm Formation: Structural Analysis and Efficacy Sulistina, Dinda Ananda; Praja, Rian Ka; Anugerahny, Margaretha Yayu Indah; Hanasia, Hanasia; Ysrafil, Ysrafil
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.921-926

Abstract

Antibiotic resistance in Staphylococcus aureus infections, especially those involving biofilm formation, is a global health issue. Biofilm protects bacteria from the immune system and antibiotic treatment, making them 10 to 1000 times more resistant. The icaD gene, part of the ica operon, is crucial for biofilm synthesis by enhancing the enzymes responsible for forming the biofilm matrix. The icaD gene sequence of Staphylococcus aureus was obtained from the GenBank NCBI database with the accession code CP140612.1, with a gene sequence length of 306 bp and employed several bioinformatics methods, including siDirect for designing and evaluating effective siRNA sequences to select the most promising candidates. Additionally, siRNA Scales, MaxExpect, Duplex Fold, and siPred were employed to analyze the siRNA sequence length, secondary structure, binding energy, and efficacy predictions of siRNAs targeting the icaD gene. The study found that out of 54 siRNA candidates, siRNA22, siRNA50, and siRNA25 achieved inhibition rates of 93.69%, 92.82%, and 92.52%, respectively. These results bioinformatically demonstrated their potential to suppress the expression of the icaD gene and highlight their promise as siRNA-based antibacterial therapies to combat biofilm-related infections. The designed siRNA computationally shows potential as an innovative therapy to combat biofilm infections caused by Staphylococcus aureus.
Co-Authors Agustira, Dewanti Angeline Novia Toemon, Angeline Novia Anugerahny, Margaretha Yayu Indah Aprianor, Dendi Caesar, Muhammad Akbar Efendy, Dennisya Azzahra Elsa Trinovita Faradila, Faradila Fathinah, Salma Fatmaria, Fatmaria Febrianti, Azizah Widyananda Furtuna, Dewi Klarita Gregorius, Ocratya Haryono, Nurul Ilma Herianto, Restu Hizawati, Hizawati Jelita, Helena Komara, Nisa Kartika Kurniawan, Mutiara Majida, Salwa Dwina Mudatama, Yusuf Muhammad Akbar Muhammad Alif Mutiarasari, Dian Nahan, Elmo Alfaliano Natalia Sri Martani Nawan, Nawan Nugraha, Yudha Purba, Abed Nego Puspita Sari, Dewi Marta Putri Welmart, Devina Vania Rakhman, Farizky Rian Ka Praja Rizaldi, M. Yusuf Rizqy, Muhammad Sulthan Sadek, Yitro Tuah HR Safitri, Andini Saputra, Aldi Aditya Sayyidinoor, Muhammad Alif Sirait, Christine Sulistina, Dinda Ananda Tiana, Devina Ananda Turnip, Oktaviani Naulita Upel, Dean Antoni Widayati, Ratna Widiarti, Astri Widyananda, Azizah Ysrafil, Ysrafil Yuliani, Ni Nyoman Sri