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All Journal Biology, Medicine, & Natural Product Chemistry Jurnal Kesehatan Medika Saintika Biosel: Biologi Science and Education BioEksakta : Jurnal Ilmiah Biologi Unsoed
Kurniadi, Helman
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Energy Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary Other

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Isolation of b-Cyclodextrin Gycosyl Transferase (b-CGTase) Producing Bacteria from Potato Plantation Soil Kurniadi, Helman; Sagita, Desi; Hartesi, Barmi
Biology, Medicine, & Natural Product Chemistry Vol 13, No 1 (2024)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2024.131.193-197

Abstract

Cyclodextrin glycosyltransferase (CGTase) is an extracellular enzyme produced by several microorganisms, particularly bacteria, that converts starch into cyclodextrin (CD). CD has various applications in fields such as food, textiles, pharmaceuticals, chemistry, and biotechnology. The objective of this study was to isolate b-CGTase-producing bacteria from soil in a potato plantation located in Kerinci, and to conduct their characterization. Screening was conducted using Horikoshi agar media with phenolphthalein and methyl orange color indicators. The results indicated that one isolate, coded CK-2, produced the optimum b-CGTase enzyme when incubated for 30 hours at 37 ºC and pH 10. The CGTase enzyme converted starch to CD when incubated for 60 minutes at 37 ºC and pH 7. The CK-2 isolate was identified as monobacilli Gram-positive bacteria, having spores, positive in the catalase test, and motile. The study concluded that the bacteria producing b-CGTase enzyme are found in the soil of potato plantations in Kerinci, which is believed to originate from the genus Bacillus sp.
Co-Authors Alfiana, Dessinta Barmi Hartesi Dahlia, Dimbi Desi Sagita Faradina Aghadiati Framisa, Mentari Hairani Heldiana, Fitria Lili Andriani Oril Ardianto Ros Sumarny Syafitri, Ulfah Eka Syamsudin Abdillah Timbul Partogi Haposan Simorangkir