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All Journal Buitenzorg: Journal of Tropical Science
Aqwin Polosoro
Research Center for Genetic Engineering, National Research and Innovation Agency
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Health Professions

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Perbandingan Metode Ekstraksi DNA pada Tanaman Ulin (Eusideroxylon zwageri) Salsabila Al Alya Ridzqya; Aqwin Polosoro; Wening Enggarini; Kusumawaty Kusumanegara; Hendra Helmanto; Mahat Magandhi; Dani Satyawan; Toto Hadiarto; Alice Yuniaty
Buitenzorg: Journal of Tropical Science Vol 1 No 1 (2024): Buitenzorg: Journal of Tropical Science
Publisher : Innovation Centre for Tropical Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70158/buitenzorg.v1i1.7

Abstract

DNA isolation is a routine procedure in molecular analysis. The method of plant genome DNA extraction has been widely available throughout global laboratories. Several labs made some modifications to obtain optimal results. This research was aimed to analyse two types of DNA extraction methods for ironwood plant (Eusideroxylon zwageri), one tropical rain forest woody plant known for its high strength and durability. The results indicate that isolation DNA kit produced high DNA purity with lower concentrations while CTAB methods generated lower DNA purity with higher concentrations. This may be used as consideration of DNA isolation for downstream molecular analyses.   Keywords: DNA extraction, ironwood, CTAB, genomic DNA
Studi perbandingan DNA barcoding untuk Bambu Seli Rizqi Awaliah; Aqwin Polosoro
Buitenzorg: Journal of Tropical Science Vol 1 No 2 (2024): Buitenzorg: Journal of Tropical Science
Publisher : Innovation Centre for Tropical Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70158/buitenzorg.v1i2.9

Abstract

The Bambusoideae subfamily, a significant group within the Poaceae family, contains diverse genera with complex taxonomic relationships. This study aimed to elucidate the phylogenetic relationships and genetic diversity within the bamboo, focusing on the utility of DNA barcoding markers, i.e., ITS2, matK, and rbcL, in bamboo species identification. By analyzing sequence data from these markers, phylogenetic trees were constructed using the maximum likelihood method to infer evolutionary relationships among species. The results showed that ITS provides the highest resolution for species-level identification, distinguishing closely related species more effectively than matK and rbcL. While matK demonstrated robust genus-level classification, rbcL was limited by its high conservation, making it more suitable for broader taxonomic groupings. These findings contribute to a better understanding of bamboo taxonomy and highlight the importance of marker selection based on the taxonomic resolution required. The study also emphasizes the complementary use of these markers to provide a comprehensive view of bamboo phylogenetics.   Keywords: bamboo taxonomy, DNA barcoding, ITS2, matK, rbcL
Co-Authors Alice Yuniaty Dani Satyawan Hendra Helmanto Kusumawaty Kusumanegara Mahat Magandhi Salsabila Al Alya Ridzqya Seli Rizqi Awaliah Toto Hadiarto Wening Enggarini