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All Journal Buitenzorg: Journal of Tropical Science
Kusumawaty Kusumanegara
Research Center for Horticulture, National Research and Innovation Agency
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Environmental Science Health Professions

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Perbandingan Metode Ekstraksi DNA pada Tanaman Ulin (Eusideroxylon zwageri) Salsabila Al Alya Ridzqya; Aqwin Polosoro; Wening Enggarini; Kusumawaty Kusumanegara; Hendra Helmanto; Mahat Magandhi; Dani Satyawan; Toto Hadiarto; Alice Yuniaty
Buitenzorg: Journal of Tropical Science Vol 1 No 1 (2024): Buitenzorg: Journal of Tropical Science
Publisher : Innovation Centre for Tropical Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70158/buitenzorg.v1i1.7

Abstract

DNA isolation is a routine procedure in molecular analysis. The method of plant genome DNA extraction has been widely available throughout global laboratories. Several labs made some modifications to obtain optimal results. This research was aimed to analyse two types of DNA extraction methods for ironwood plant (Eusideroxylon zwageri), one tropical rain forest woody plant known for its high strength and durability. The results indicate that isolation DNA kit produced high DNA purity with lower concentrations while CTAB methods generated lower DNA purity with higher concentrations. This may be used as consideration of DNA isolation for downstream molecular analyses.   Keywords: DNA extraction, ironwood, CTAB, genomic DNA
Co-Authors Alice Yuniaty Aqwin Polosoro Dani Satyawan Hendra Helmanto Mahat Magandhi Salsabila Al Alya Ridzqya Toto Hadiarto Wening Enggarini