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Journal : BIOTROPIC

The Detection of the Escherichia coli Gene in Well Water in Bojonegoro Using Polymerase Chain Reaction (PCR) Prayekti, Endah; Rosiana Dewi Regita; Khiliah Navis
Biotropic : The Journal of Tropical Biology Vol. 10 No. 1 (2026): Biotropic, Volume 10 Nomor 1, 2026
Publisher : Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Sunan Ampel Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29080/biotropic.v10i1.2449

Abstract

ABSTRACT Escherichia coli (E. coli) is an indicator of water contamination that can be pathogenic, especially the Enterotoxigenic E. coli (ETEC) pathotype, which carries the heat-resistant enterotoxin (est) gene. Well water that does not meet clean water quality standards can become a medium for Escherichia coli contamination, so it is important to perform molecular genetic detection. The study aimed to determine the presence of Escherichia coli bacteria and detect the est gene from well water isolates using the Polymerase Chain Reaction (PCR) method. Samples were collected from 19 wells using purposive sampling techniques. Escherichia coli identification was performed through conventional isolation, Gram staining, biochemical tests, and culture on selective media (EMB and MCA). The results of this study showed that four samples tested positive for Escherichia coli. Molecular detection using PCR for the est gene in Escherichia coli was not detected in any of the four Escherichia coli isolates. Based on these results, it was concluded that Escherichia coli was present in the samples, but the est gene was not detected. Keywords: Escherichia coli, est gene, well water, PCR ABSTRAK Escherichia coli (E. coli) merupakan indikator pencemaran air yang dapat bersifat patogen, terutama patotipe Enterotoxigenic E. coli (ETEC) yang membawa gen enterotoksin tahan panas (est). Air sumur yang tidak memenuhi standar kualitas air bersih dapat menjadi media kontaminasi Escherichia coli, sehingga penting dilakukan deteksi genetik secara molekuler. Penelitian bertujuan untuk mengetahui keberadaan bakteri Escherichia coli dan mendeteksi gen est dari isolat air sumur menggunakan metode Polymerase Chain Reaction (PCR). Sampel diambil dari 19 sumur menggunakan teknik purposive sampling. Identifikasi Escherichia coli dilakukan melalui isolasi konvensional, pewarnaan Gram, uji biokimia, dan kultur pada media selektif (EMB dan MCA). Hasil penelitian ini menunjukkan adanaya 4 sampel yang memiliki positif Escherichia coli. Deteksi molekuler dengan PCR terhadap gen est pada Escherichia coli tidak ditemukan pada keempat isolat Escherichia coli. Berdasarkan hasil penelitian ini disimpulkan bahwa pada sampel ditemukan Escherichia coli tetapi tidak ditemukan gen est. Kata Kunci: Escherichia coli, gen est, air sumur, PCR
Co-Authors Akbar Reza Muhammad Akhmad, Nur Faradila Andini, Ary Andreas Putro Ragil Santoso, Andreas Putro Ragil Ardyarini Dyah Savitri Arsya Tazkiya Awaliyah, Arisma Putri Baihaqi, Muhammad Azhari Brina Thursina Dibiasi Devita Rahma Putri Devyana Dyah Wulandari dr Hartatiek Nila,Sp.OG Elsya Octaviani Cantika Putri Endah Budi Permana Putri Ersalina Nidianti Eva Rosa Dwi Febrianti Evi Sylvia Awwalia Fadillah Triasmoro Fakurazi, Sharida Fauziah, Luqiana Gilang Nugraha Hamiduumajid Ballihg Ballihgoo Hamiduumajid Ballihg Ballihgoo Hermawati, Septina Isro’ Hidayat, Muhammad Taufiq Husein Firdaus Ibrahim Dwi Waluyo Putra Indah Nur Kamaliyah Indaryanti, Reski Novita Junaedy, M Dwinanda Kamelia, Dina Kardina, Rizki Nurmalya Khiliah Navis Khusnah, Hidayatul Kifli, Nurolaini M Nasir Maat, Suprapto Maghfuroh, Qonita Iklila Mardhotillah, Rachma Rizqina Marinda Dwi Puspitarini Matin, Nur Sophia Melindah, Winda Mirshall Abiedama Prayoga Mochamad Faishal Riza Muhammad Afwan Romdloni Muhammad Afwan Romdloni Muhammad Taufiq Hidayat Muhammad Taufiq Hidayat Mukarramah, Imraatul Mutiarawati, Diah Titik Nada Mufarrohah Nandasari, Anggy Dwipa Nathalya Dwi Kartika Sari Navis, Khiliah Ngiluhtara Aditiya Putri Nisa, Fauziyatun Nunki, Nastasya Nur Naili Fahira Pratama, Nizar Revandini Prayoga, Mirshall Abiedama Prisnidiawati, Ajeng Puspitasari, Mia Putri Lestari, Ajeng Anindhita Putri, Ananda Ghufron Yuana Putri, Nasya Desinta Qotrinnada, Nabilah Rahmawati, Lili Susan Ramadani, Vania Nur Regina Ayu Fristiyanti Rhomadhoni , Muslikha Nourma Riza, Mochamad Faishal Riza, Mochammad Faishal Rizki Jalil, Ike Wasilatur Rizki Nurmalya Kardina Romdhoni, Muhammad Afwan Rosiana Dewi Regita Sa'bania Hari Raharjeng Sabrina, Shinta Milenia Sadza, Lailatus Salfa Salsabilah Zain Salimi, Syafrina Maulidyatus Salman Firmansyah Sari, Devi Fitriana Satya Nugraha Wirayudha Seggaf Achmad Syah Setiarsih, Dini Setyana Yuli, Azizah Ari Siti Husnul Khotimah Sofaria Munir, Ratna Sonia Fitriatul Qolbi Suliati Suliati Sunaryo, Merry Suryandaru, Suryandaru Syafiuddin, Achmad Tauziah, Amalia Thomas Sumarsono Thomas Sumarsono Thomas Sumarsono Tullah, Auria Hidayah Uliyatul Laili, Uliyatul Umaha, Farida Wandara Sekar Ayu Pramesti Wideistianti, Annisa Pricilya Widya Novita Sari Wiwik Winarningsih Wulan, Wieke Sri Wulandari, Dwi Agustin Yauwan Tobing Lukiyono Yuliana Yuliana Yuni Nur Malita Sari Yuwono, Asma' Denaya Psari Zahrotul Illiyin Zain, R.A Wafiqah Aulia Zalfa, Anisatuz Zazabilla, Indira