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All Journal Jurnal Penelitian Hasil Hutan Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Riset Industri Hasil Hutan Jurnal Hutan dan Masyarakat Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Agrotek UMMat SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Biosaintifika: Journal of Biology & Biology Education EMBRIO : Jurnal Kebidanan Logista: Jurnal Ilmiah Pengabdian Kepada Masyarakat IJEEM - Indonesian Journal of Environmental Education and Management Jurnal Kridatama Sains dan Teknologi ULIN: Jurnal Hutan Tropis Jurnal Abdi Insani Jurnal Penelitian Hasil Hutan Journal of Forest Science Avicennia Lumbung Inovasi: Jurnal Pengabdian Kepada Masyarakat Jurnal Silva Samalas: Journal of Forestry and Plant Science Jurnal Ilmiah Sangkareang Mataram Empiricism Journal Journal of Agritechnology and Food Processing Rona Teknik Pertanian Prosiding Seminar Nasional Unimus Biosaintifika: Journal of Biology & Biology Education International Journal of Ethnoscience and Technology in Education Jurnal Penelitian Hasil Hutan Sasambo: Jurnal Abdimas (Journal of Community Service)
Wangiyana, I Gde Adi Suryawan
Program Studi Kehutanan Universitas Pendidikan Mandalika
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Journal : Jurnal Ilmiah Sangkareang Mataram

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DEVELOPMENT OF INDUCTION TECHNOLOGY ON AGARWOOD CULTIVATION - A REVIEW I Gde Adi Suryawan Wangiyana
JURNAL SANGKAREANG MATARAM Vol. 6 No. `1 (2020): Maret 2020
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Agarwood is one of high valuable non-timber forest commodity produce by family thymeleaceae, especially genus Aquilaria and Gyrinops. Agarwood commodity on the market only could be sold from cultivated species. Thus, agarwood cultivation method should be fully understood by agarwood farmers and agarwood researchers. Induction technology is one of the most important things on agarwood cultivation method. Induction procedure could induce resin production on agarwood cultivated species. This resin has an aromatic fragrance which well known as “gubal” on the market. It is imposible for cultivated agarwood species to produce gubal without induction process. Induction tehnology has been developed form old traditional method into new modern method. Each method has seraval advantages and disadvantages. Old traditional method induced agarwood physically using traditional equipment such as: machete, chopper and nail. It is simple method but only produce resin in very small area. Modern method induces agarwood using chemical liquid or microorganism culture that could spread all over agarwood stem. This method could induce resin in a very wide area compare to traditional method. Resin productivity using modern method is higher than those traditional methods. Thus this modern method induction technology could be best solution for agarwood farmers to increase their agarwood resin.
SIMILARITY ANALYSIS OF GENERA AQULARIA AND GYIRINOPS BASED ON VEGETATIVE STRUCTURE FEATURE USING DIFFERENT CLUSTERING METHOD I Gde Adi Suryawan Wangiyana
JURNAL SANGKAREANG MATARAM Vol. 5 No. 1 (2019): Maret 2019
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The purpose of this research is to analysis similarity of Aquilaria group and Gyrinops group based on several feature of vegetative organ. Member of Aquilaria Group and Gyrinops group were selecting as OTU based on their validation nomenclature from international taxonomy database. Dendrograms were constructed using MVSP version 3.1A. UPGMA Algorithm were used for clustering method. Two similarity index, Simple Matching Coefficient and Jaccard Coefficient, were used for distance measurement on dendrogram. Cophenetic correlation analysis between similarity matrix were conducted by CoStat Cohort 6. Similarity matrix based on simple matching coefficient were different from similarity matrix based on Jaccard Coefficient on the aspect of OTU pair. Simple Matching coefficient also have higher similarity index then Jaccard coefficient. Moreover, Dendrogram topology based on simple matching coefficient were different from dendrogram topology based on Jaccard coefficient on aspect of sister taxa pairing complex. Cophenetic correlation analysis confirm that distortion data between all similarity matrix were not significant. That means, dendrogram based on these clustering methods were accurate and reliable. It could be concluded that Aquilaria group and Gyrinops group has high similarity index which make them randomly clustering in the same cluster based on its vegetative character feature.
CHARACTERISTIC OF AGARWOOD TEA FROM GYRINOPS VERSTEEGII FRESH AND DRY LEAVES I Gde Adi Suryawan Wangiyana; Sami'un
JURNAL SANGKAREANG MATARAM Vol. 4 No. 2 (2018): Juni 2018
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Gyrinops versteeegii in Lombok Island mainly be focus on cultivating of agarwood gubal commodity which need up to 14 years for production. Use the leaf to make a tea is one of potential alternative commodity form G. versteegii which give guarantee for agarwood farmers to have a continuous product from their plantation. The purpose of this research is to study different characteristic of tea from fresh and dry agarwood leaves. Leaves sample were taken from the branch near the shoot of G. versteegii tree. After being washed multiple times, 20 gr of fresh leaves were boiled in1 L of 100oC water for 5 minutes. Ten gram of Dry leaves after losing 60% of its water content was brewed in 1 L of 100oC water for 5 minutes. Both fresh leaf tea and dry leaf tea were added with 7,5% w/v sucrose. Fresh leaf tea and Dry leaf tea have a different color, brightness, and turbidity. These 2 types of tea also different in odor and taste. Dry leaf tea has a strong odor and bitter taste while fresh leaf tea has a light odor and weak taste. Slighly bitter taste from dry leaf tea possibly caused by tannin. It could be concluded that agarwood tea from fresh leaf and dry leaf has a different appearance, odor, and taste. Study about phytochemistry of agarwood tea and the hedonic test is needed for better understanding about agarwood tea.
PHYLOGENETIC ANALYSIS OF AQUILARIA AND GYRINOPS MEMBER BASED ON TRNL-TRNF GENE SEQUENCE OF CHLOROPLAST I Gde Adi Suryawan Wangiyana
JURNAL SANGKAREANG MATARAM Vol. 2 No. 4 (2016): Desember 2016
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Aquilaria and Gyrinops are main agarwood producers that only could be distinguished based on their flower structure. Molecular characteristic analysis is needed to support morphological character for breeding and conservation purpose. Several molecular analyses were conducted based on similarity characteristic of Aquilaria and Gyrinops. However phylogenetic analysis of these genera was so few to support the polyphasic classification. The purpose of this study is to do phylogenetic analysis based on trnL-trnF sequence. Thirteen sequences of Aquilaria and six sequences of Gyrinops were downloaded from NCBI. ClustalX 2.1 program were used for multiple alignment of sequences. Three phylogenetic trees were reconstructed with MEGA 5.1 by different method including: Neighbor Joining, Minimum and Maximum Liklihood. Phydit program were used to construct similarity matrix between sequences. Based on phylogenetic analysis, all Aquilaria members were clustered in the same clade. All sister taxa also composed with Aquilaria member except for A.hirta. On the other hand, G.salicifora, G.Podocarpa and G.Ladermanii are the only Gyrinops members that form same clade. Other Gyrniops members were spread to different clade. It could be concluded that trnL-trnF sequence is a useful phylogenetic marker for Aquilaria although have several limited for Gyrinops. Moreover, based on phylogenetic analysis and similarity analysis it could be suggested that Aquilaria and Gyrinops sould not be separated into different genera.
PEMANFAATAN MEDIUM ALTERNATIF UNTUK PERTUMBUHAN ISOLAT FUSARIUM SP. PENGINDUKSI PEMBENTUKAN GAHARU PADA GYRINOPS VERSTEEGII (GILG) DOMKE I Gde Adi Suryawan Wangiyana
JURNAL SANGKAREANG MATARAM Vol. 1 No. 3 (2015): Desember 2015
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Gaharu terbentuk di dalam batang Gyrinops versteegii melalui bio-induksi oleh kapang Fusarium sp. Keberhasilan Fusarium menginduksi pelepasan resin sebagai bahan dasar gaharu sangat tergantung pada berbagai faktor. Medium pertumbuhan jamur menentukan kualitas gaharu yang diinduksi pada G. versteegii. Penelitian ini bertujuan untuk melakukan seleksi isolat Fusarium yang mampu menginduksi pembentukan gaharu berdasarkan kemampuan tumbuh dan produksi konidia pada berbagai medium alternatif. Medium alternatif yang digunakan diantaranya: medium serbuk kayu, medium sebuk kayu G. versteegii, medium tauge serta medium kentang (PDA) yang digunakan sebagai kontrol. Sementara itu, isolat Fusarium yang digunakan diantaranya: LU, ALS, LT, LB dan BIMA. Berdasarkan data pertambahan diameter koloni, pertumbuhan tiap isolat pada medium tauge lebih baik dibandingkan PDA. Produksi konidia tiap isolat pada medium tauge juga lebih tinggi dibandingkan medium kontrol PDA. Rerata diameter koloni dan produksi spora tiap isolat pada medium kayu pre-treatment akuades paling mendekati PDA (kontrol). Isolat BIMA merupakan isolat yang memiliki pertumbuhan terbaik dan produksi konidia terbesar dibandingkan isolat lainnya. Data diameter koloni dan produksi konidia tiap medium berbeda secara signifikan berdasarkan ANOVA dan uji lanjut BNJ. oleh karena itu dapat disimpulkan bahwa media tauge dan kayu pre-treatment akuades merupakan medium alternatif terbaik dan sangat potensial digunakan sebagai media inokulan Fusarium penginduksi gaharu. Sementara itu, isolat BIMA merupakan isolat dengan pertumbuhan terbaik yang potensial untuk dikembangkan lebih lanjut sebagai agensia bio-induksi gaharu.
MOLECULAR PHYLOGENETIC ANALYZE OF FUSARIUM FROM AGARWOOD AND OTHERS FUSARIUM WITH DIFFERENT TYPE OF NUTRITION BASED ON GEN ITS 1 I Gde Adi Suryawan Wangiyana
JURNAL SANGKAREANG MATARAM Vol. 2 No. 1 (2016): Maret 2016
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Fusarium is a common mold used as inoculants for inducing resin formation in agarwood tree. Molecular phylogenetic analyze is important to be done to determine the correlation between type of nutrition of Fusarium and its ability to associated with Agarwood. ITS 1 gene sequences were downloaded from NCBI gene bank was used as sequences to reconstruct phylogenetic tree. ClustalX 2.1 program were used for multiple alignment of sequences. Two phylogenetic tree, Neighbor Joining and Maximum Likelihood, were reconstructed with MEGA 5.1 program. One phylogenetic tree were reconstructed with mrbayes program using Markov Chain Monte Carlo method. Phydit program were used to construct similarity matrix between sequences.Different type of nutrition on Fusarium including: endophytic, saprophytic and phytopathogenic were not a monophyletic based on phylogenetic tree analyze. That type of nutrition was formed by adaptation process. They do not share that characteristic withtheir common ancestor. Fusarium from agarwood has the same clade with phytopathogenic Fusarium in all phylogenetic trees. They also have the highest ITS 1 gene sequence similarity with phytopathogenic Fusarium. Therefore, it could be concluded that Fusarium from agarwood are phytopathogenic group and they have pathogen-host association with agarwood.
INTERACTION OF FUSARIUM SP. WITH GYRINOPS VERSTEEGII SEEDLING BY MORPHOLOGICAL, ANATOMICAL, AND CHEMICAL OBSERVATION I Gde Adi Suryawan Wangiyana
JURNAL SANGKAREANG MATARAM Vol. 3 No. 3 (2017): September 2017
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Studi of interaction between Gyrinops versteegii and Fusarium Sp. could lead advance understanding in agarwood cultivation. The aim of this research is to observe interaction between Fusarium Sp. and Gyrinops versteegii based on morphological, anatomical and chemical observation. Gyrinops versteegii seedling from Lingsar was inoculated by Fusarium sp using grafting method. Morphological observation conducted by observation of chlorosis in leaf and dark colorization in stem. Anatomical observation conducted by observation of seedling stem tissue. Chemical observation conducted by thin layer chromatography. Based on morphological observation, chlorosis of leaf occurred after 14 days inoculation followed by necrosis of leaf at 21 days after inoculation. Penetration of fungal mycelia, fungal conidia and formation of dark coloration on seedling tissue were observed from anatomical observation. Similar spot with similar Rf (0.22) value were observe from seedling sample at 14 days inoculation, 21 days inoculation and also sample of Gyrinops versteegii 6 month after inoculation. All of those spots have similar Rf with standard agarwood oil (Rf = 0.23). Organoleptic test confirmed aromatic odor from seedling at 14 days and 21 day inoculation even though the aromatic odor is not as strong as aromatic odor of Gyrinops versteegii tree at 6 month inoculation. It could be concluded that the inoculation of Fusarium sp. on Gyrinops versteegii resulting: chlorosis and necrosis of leaf, dark colorization of stem, aromatic odor and identical Rf spot of Thin Layer Chromatography.
TEH GYRINOPS: PRODUK TEH HERBAL HUTAN UNGGULAN PULAU LOMBOK I Gde Adi Suryawan Wangiyana
JURNAL SANGKAREANG MATARAM Vol. 8 No. 3 (2021): September 2021
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Teh herbal hutan adalah seduhan organ daun tanaman hutan yang didominasi oleh tanaman habitus pohon. Teh herbal hutan ini dapat menjadi bentuk diversifikasi produk kehutanan yang potensial untuk dikembangkan. Teh Gyrinops merupakan teh gaharu jenis baru yang dikembangkan di Pulau Lombok dari spesies Gyrinops versteegii. Umumnya teh gaharu diproduksi dari kelompok Aquilaria, sehingga pengembangan teh gaharu dari spesies G. versteegii masih sangat potensial untuk dilakukan. Produk teh Gyrinops diolah dari bahan baku daun G. versteegii yang selama ini menjadi limbah dalam budidaya gaharu melalui proses pruning. Sebagai tambahan, pengolahan daun G. versteegii menjadi teh Gyinops membutuhkan waktu investasi yang singkat karena dapat dipanen sejak pohon gaharu berusia kurang dari 5 tahun. Waktu investasi singkat serta dapat dipanen secara berkelanjutan menjadikan pengolahan daun G. versteegii menjadi teh Gyrinops mampu memberikan tambahan pemasukan bagi petani gaharu Lombok selama masa tunggu produksi resin gaharu yang menjadi komoditi utama dalam budidaya gaharu. Untuk pengembangan produk teh Gyrinops menjadi teh herbal unggulan dari pulau Lombok diperlukan studi intensif terhadap faktor – faktor pendukungnya. Faktor – faktor tersebut diantaranya seleksi bahan baku, optimalisasi produksi, standardisasi dalam aspek fitokimia, serta penambahan bahan herbal untuk menghasilkan produk yang unik.
APLIKASI BLAST NCBI DALAM PENCARIAN TYPE REFERENCE SEKUEN RBCL Gyrinops versteegii I Gde Adi Suryawan Wangiyana
JURNAL SANGKAREANG MATARAM Vol. 9 No. 1 (2022): Maret 2022
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Tujuan riset ini adalah untuk menguji aplikasi BLAST NCBI dalam pencarian type reference sekuen rbcL spesies Gyrinops versteegii sekaligus sebagai acuan analisis filogenetik. Daun G. versteegii diambil dari perkebunan gaharu di Desa Langko Lombok Barat. Ekstraksi DNA dari daun G. versteegii dilakukan dengan menggunakan Blood Animal Plant DNA Preparation Kit. DNA genom hasil isolasi diukur konsentrasi dan kemurniannya dengan mengukur absorbansi pada panjang gelombang 230 nm, 260 nm, dan 280 nm. Amplifikasi PCR dilakukan dengan menggunakan KAPA 2G PCR mix dengan program initial denaturation 95 http://www.sangkareang.org/ Volume 9,No.1,Maret 2022 o C selama 3 menit diikuti 40 siklus denaturasi 95 o C selama 15 detik, annealing 37 o C selama 1 menit, extension 72 o C selama 2 menit, dan final extension 72 o C selama 5 menit. Penelurusan Type reference dilakuakn dengan menggunakan aplikasi MegaBLAST. Hasil Penelusuran selanjutnya di alignment dengan ClustalX dan direkonstruksi pohon filogenetiknya menggunakan alogritme Neighbor-Joining dan Maximum Parsimony pada program MEGA 5.1. Hasil penelurusan MegaBLAST menunjukkan type reference rbcL G. versteegii yang terdiri dari 13 genus dan 43 spesies. Beberapa genus merupakan anggota family Thymeleaceae (Gyrinops, Aquilaria, Gonystylus, dan Wikstroemia) namun terdapat pula genus non family Thymeleaceae (Degeworthia, Stellera, Daphne, Diarthorn, Phaleria, Pimelea, Ovidia, Gnidia, dan Lachnaea). Genus Gyrinops dan Aquilaria tetap menjadi dua genus dengan nilai % identity tertinggi sehingga dijadikan type reference untuk rekonstruksi pohon filogenetik. Pohon filogenetik algoritme Neighbor-Joining maupun Maximum Parsimony sama – sama menunjukkan bahwa OTU G. versteegii selalu terkumpul dalam clade yang sama. Hal ini sekaligus menunjukkan hubungan yang bersifat monofiletik.
REKONSTRUKSI POHON FILOGENETIK DARI SEKUEN MATURASE K GYRINOPS VERSTEEGII MENGGUNAKAN REFERENSI PENCARIAN MEGABLAST I Gde Adi Suryawan Wangiyana
JURNAL SANGKAREANG MATARAM Vol. 9 No. 2 (2022): Juni 2022
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Penelitian ini bertujuan untuk melakukan analisis filogenetik terhadap sekuen matK Gyrinops versteegii berdasarkan pencarian reference menggunakan MegaBLAST dari NCBI. Sampel diambil dari tegakan pohon G. versteegii di perkebunan gaharu Desa Langko Lombok Barat. Blood Animal Plant DNA Preparation Kit digunakan untuk ekstraksi DNA dari organ daun. Pengukuran absorbansi panjang gelombang 230 nm, 260 nm, dan 280 nm dilakukan pasca ekstraksi DNA. Amplifikasi PCR dilakukan dengan menggunakan KAPA 2G PCR mix dengan program initial denaturation 95oC selama 3 menit diikuti 40 siklus denaturasi 95oC selama 15 detik, annealing 37oC selama 1 menit, extension 72oC selama 2 menit, dan final extension 72oC selama 5 menit. Pencarian type reference dilakukan menggunakan MegaBLAST dan dilakukan alignment sekuen dengan menggunakan ClustalX. Rekonstruksi pohon filogenetik dilakukan menggunakan program MEGA 5.1. dengan dua algoritme filogenetik yaitu Neighbor Joining dan Maximum Parsimony. Hasil penelusuran MegaBLAST menunjukkan sekuen matK G. versteegii mengerucut pada dua genus dalam family Thymeleaceae yaitu: Aquilaria dan Gyrinops. Pohon filogenetik yang direkonstruksi dengan algoritme berbeda memiliki topologi yang berbeda. G. versteegii berkumpul dalam satu clade sebagai sister taksa pada pohon filogenetik Neighbor Joining namun terpisah pada klade berbeda pada pohon filogenetik Maximum Parsimony. Tergabungnya Gyrinops dan Aquilaria dalam beberapa klade yang sama menunjukkan bahwa kedua genus ini memiliki hubungan kekerabatan yang dekat. Dapat disimpulkan bahwa sekuen matK G. versteegii berdasarkan penelusuran MegaBLAST merupakan marker ideal yang digunakan untuk melakukan analisis filogenetik genus Gyrinops dan Aquilaria yang bersifat monofiletik.
Co-Authors . Akram Aini, Husnul Akhmad Sukri Akram Akram, . Amelia Kandisa Anita Nugraheni, Yosephin Martha Maria Anri Anri Arbi Sanjaya Baharudin Baharudin Cep Ahmad Muhtar Dewi, Ni Putu Ety Diana Hidayati Efendi Iskandar Elly Purwanti Farouq Isbulloh Fathurrahman Fathurrahman Fatihatum, Fatihatum Gunawan, Frendi Hafiz, Yusron Hairani Hairani Hendriko, Albert Hidayah, Zoya Saharani Hidayat Wicaksono I Gde Dharma Atmaja I Gusti Agung Ayu Hari Triandini Irama, Ade Isbulloh, Farouq Isviyanti Isviyanti Kemas Usman Kesumajayadi, Lalu Kurnia, Nova Lutfi Anggadhania Lutfi Anggadhania Malik, Sukardi Masrilurrahman, LL. Suhirsan Mirawati, Baiq Nahlunnisa, Hafizah Ni Made Gita Gumangsari Ni Made Gita Gumangsari Nova Kurnia Putri, Dina Soes Putu Surya Wedra Lesmana Raden Roro Narwastu Dwi Rita Radjali Amin Rahma Ziska Rosa Martadiana Sad Kurniati Wanitaningsih Sad Kurniati Wanitaningsih Sad Kurniati Wanitaningsih Sadir, Muhammad Salman Al Farisi, Salman Al Sami'un Sarowan Wahid Sembiring, Rinawati Sukardi Malik Sumarlin, Muhamad Wahyu Prihanta Wahyu Yuniati Nizar Wanitaningsih, Sad Kurniati Wayan Wangiyana Wiranto Wiranto Wulandari, Febriana Wulandari, Febriana Tri Yani Mulyani Yayan Suherna Putri Yulia Ratnaningsih Yulia Ratnaningsih Yulia Ratnaningsih Zarkasi Nur